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. 1987 Aug;56(2):127–132. doi: 10.1038/bjc.1987.169

Modulation of placental alkaline phosphatase activity and cytokeratins in human HN-1 cells by butyrate, retinoic acid, catecholamines and histamine.

J T Bijman 1, D J Wagener 1, H van Rennes 1, J M Wessels 1, F C Ramaekers 1, P van den Broek 1
PMCID: PMC2002146  PMID: 2444242

Abstract

The effects of butyrate and retinoic acid in combination with catecholamines or histamine on the HN-1 human head and neck squamous carcinoma cell line were investigated analysing cell proliferation, placental alkaline phosphatase (PLAP) activity, and relative cytokeratin content. Butyrate inhibited cell proliferation in agar, whereas retinoic acid induced a small inhibitory effect. Butyrate enhanced PLAP activity in a time related manner in contrast to retinoic acid, which had no significant effect. However, retinoic acid inhibited the efficacy of butyrate to induce PLAP activity. A synergistic enhancement of PLAP activity was demonstrated after treatment of butyrate pretreated cells with catecholamines or histamine. The beta-adrenergic antagonist propranolol partly inhibited the aforementioned enhancement of PLAP activity, whereas the alpha-adrenergic antagonist phentolamine further enhanced PLAP activity. Indirect labeling of keratins with a polyclonal antibody showed that cytokeratin content was enhanced by butyrate but not by retinoic acid. Further analysis of cytokeratin content using four monoclonal antibodies showed that labeling of cytokeratins (5 + 8) was increased by butyrate. PLAP activity could be modulated by a concerted action of either butyrate plus retinoic acid or butyrate plus catecholamines or histamine, indicating a possible role for PLAP in tumour cell proliferation.

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Selected References

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  1. Abe M., Kufe D. W. Effect of sodium butyrate on human breast carcinoma (MCF-7) cellular proliferation, morphology, and CEA production. Breast Cancer Res Treat. 1984;4(4):269–274. doi: 10.1007/BF01806038. [DOI] [PubMed] [Google Scholar]
  2. Augeron C., Laboisse C. L. Emergence of permanently differentiated cell clones in a human colonic cancer cell line in culture after treatment with sodium butyrate. Cancer Res. 1984 Sep;44(9):3961–3969. [PubMed] [Google Scholar]
  3. Bijman J. T., Wagener D. J., van Rennes H., Wessels J. M., van den Broek P. Flow cytometric evaluation of cell dispersion from human head and neck tumors. Cytometry. 1985 Jul;6(4):334–341. doi: 10.1002/cyto.990060410. [DOI] [PubMed] [Google Scholar]
  4. Burke D. C. Interferon and cell differentiation. Br J Cancer. 1986 Mar;53(3):301–306. doi: 10.1038/bjc.1986.52. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cowley G. P., Smith J. A., Gusterson B. A. Increased EGF receptors on human squamous carcinoma cell lines. Br J Cancer. 1986 Feb;53(2):223–229. doi: 10.1038/bjc.1986.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cruise J. L., Cotecchia S., Michalopoulos G. Norepinephrine decreases EGF binding in primary rat hepatocyte cultures. J Cell Physiol. 1986 Apr;127(1):39–44. doi: 10.1002/jcp.1041270106. [DOI] [PubMed] [Google Scholar]
  7. Easty D. M., Easty G. C., Carter R. L., Monaghan P., Butler L. J. Ten human carcinoma cell lines derived from squamous carcinomas of the head and neck. Br J Cancer. 1981 Jun;43(6):772–785. doi: 10.1038/bjc.1981.115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fuchs E., Green H. Regulation of terminal differentiation of cultured human keratinocytes by vitamin A. Cell. 1981 Sep;25(3):617–625. doi: 10.1016/0092-8674(81)90169-0. [DOI] [PubMed] [Google Scholar]
  9. Henneberry R. C., Smith C. C., Tallman J. F. Relationship between beta-adrenergic receptors and adenylate cyclase in HeLa cells. Nature. 1977 Jul 21;268(5617):252–254. doi: 10.1038/268252a0. [DOI] [PubMed] [Google Scholar]
  10. Jetten A. M. Modulation of cell growth by retinoids and their possible mechanisms of action. Fed Proc. 1984 Jan;43(1):134–139. [PubMed] [Google Scholar]
  11. Kamech N., Hill A. M., Seif R., Pantaloni D. Butyrate converts rat 3T3 fibroblasts into giant cells. Exp Cell Res. 1986 Feb;162(2):326–334. doi: 10.1016/0014-4827(86)90338-1. [DOI] [PubMed] [Google Scholar]
  12. Kennedy M. F., Tutton P. J., Barkla D. H. Adrenergic factors regulating cell division in the colonic crypt epithelium during carcinogenesis and in colonic adenoma and adenocarcinoma. Br J Cancer. 1985 Sep;52(3):383–390. doi: 10.1038/bjc.1985.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kim K. H., Schwartz F., Fuchs E. Differences in keratin synthesis between normal epithelial cells and squamous cell carcinomas are mediated by vitamin A. Proc Natl Acad Sci U S A. 1984 Jul;81(14):4280–4284. doi: 10.1073/pnas.81.14.4280. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kitzis A., Tichonicky L., Defer N., Kruh J. Effect of sodium butyrate on chromatin structure. Biochem Biophys Res Commun. 1980 Apr 14;93(3):833–841. doi: 10.1016/0006-291x(80)91152-3. [DOI] [PubMed] [Google Scholar]
  15. Mary P. L., Rao J. P. Type of adrenoceptors involved in induction by adrenaline of hepatic alkaline phosphatase in mice in vivo. Br J Pharmacol. 1981 Jan;72(1):8–9. doi: 10.1111/j.1476-5381.1981.tb09096.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mier P. D., van Rennes H. Cutaneous alkaline phosphatase: a biochemical study. Arch Dermatol Res. 1982;274(3-4):221–227. doi: 10.1007/BF00403725. [DOI] [PubMed] [Google Scholar]
  17. Mier P. D., van Rennes H., van Erp P. E., Roelfzema H. Cutaneous sialidase. J Invest Dermatol. 1982 Apr;78(4):267–269. doi: 10.1111/1523-1747.ep12506847. [DOI] [PubMed] [Google Scholar]
  18. Moll R., Franke W. W., Schiller D. L., Geiger B., Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 1982 Nov;31(1):11–24. doi: 10.1016/0092-8674(82)90400-7. [DOI] [PubMed] [Google Scholar]
  19. Morita A., Tsao D., Kim Y. S. Effect of sodium butyrate on alkaline phosphatase in HRT-18, a human rectal cancer cell line. Cancer Res. 1982 Nov;42(11):4540–4545. [PubMed] [Google Scholar]
  20. Nakagawa T., Nakao Y., Matsui T., Koizumi T., Matsuda S., Maeda S., Fujita T. Effects of sodium n-butyrate on alpha-fetoprotein and albumin secretion in the human hepatoma cell line PLC/PRF/5. Br J Cancer. 1985 Mar;51(3):357–363. doi: 10.1038/bjc.1985.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pierce G. B., Wallace C. Differentiation of malignant to benign cells. Cancer Res. 1971 Feb;31(2):127–134. [PubMed] [Google Scholar]
  22. Prasad K. N. Butyric acid: a small fatty acid with diverse biological functions. Life Sci. 1980 Oct 13;27(15):1351–1358. doi: 10.1016/0024-3205(80)90397-5. [DOI] [PubMed] [Google Scholar]
  23. Prasad K. N., Sinha P. K. Effect of sodium butyrate on mammalian cells in culture: a review. In Vitro. 1976 Feb;12(2):125–132. doi: 10.1007/BF02796360. [DOI] [PubMed] [Google Scholar]
  24. Ramaekers F. C., Beck H., Vooijs G. P., Herman C. J. Flow-cytometric analysis of mixed cell populations using intermediate filament antibodies. Exp Cell Res. 1984 Jul;153(1):249–253. doi: 10.1016/0014-4827(84)90467-1. [DOI] [PubMed] [Google Scholar]
  25. Ramaekers F. C., Puts J. J., Moesker O., Kant A., Huysmans A., Haag D., Jap P. H., Herman C. J., Vooijs G. P. Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumours: an overview. Histochem J. 1983 Jul;15(7):691–713. doi: 10.1007/BF01002988. [DOI] [PubMed] [Google Scholar]
  26. Reese D. H., Gratzner H. G., Block N. L., Politano V. A. Control of growth, morphology, and alkaline phosphatase activity by butyrate and related short-chain fatty acids in the retinoid-responsive 9-1C rat prostatic adenocarcinoma cell. Cancer Res. 1985 May;45(5):2308–2313. [PubMed] [Google Scholar]
  27. Reese D. H., Politano V. A. Evidence for the retinoid control of urothelial alkaline phosphatase. Biochem Biophys Res Commun. 1981 Sep 16;102(1):322–327. doi: 10.1016/0006-291x(81)91524-2. [DOI] [PubMed] [Google Scholar]
  28. Reiss M., Pitman S. W., Sartorelli A. C. Modulation of the terminal differentiation of human squamous carcinoma cells in vitro by all-trans-retinoic acid. J Natl Cancer Inst. 1985 May;74(5):1015–1023. [PubMed] [Google Scholar]
  29. Riggs M. G., Whittaker R. G., Neumann J. R., Ingram V. M. n-Butyrate causes histone modification in HeLa and Friend erythroleukaemia cells. Nature. 1977 Aug 4;268(5619):462–464. doi: 10.1038/268462a0. [DOI] [PubMed] [Google Scholar]
  30. Sartorelli A. C. The 1985 Walter Hubert lecture. Malignant cell differentiation as a potential therapeutic approach. Br J Cancer. 1985 Sep;52(3):293–302. doi: 10.1038/bjc.1985.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Schiff L. J., Moore S. J. Effects of cyclic adenosine 3':5'-monophosphate-elevating agents and retinoic acid on differentiation in retinoid-deficient tracheal cultures. In Vitro Cell Dev Biol. 1985 Dec;21(12):688–692. doi: 10.1007/BF02620923. [DOI] [PubMed] [Google Scholar]
  32. Tallman J. F., Smith C. C., Henneberry R. C. Induction of functional beta-adrenergic receptors in HeLa cells. Proc Natl Acad Sci U S A. 1977 Mar;74(3):873–877. doi: 10.1073/pnas.74.3.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Tutton P. J., Barkla D. H. Neural control of colonic cell proliferation. Cancer. 1980 Mar 15;45(5 Suppl):1172–1177. doi: 10.1002/1097-0142(19800315)45:5+<1172::aid-cncr2820451322>3.0.co;2-b. [DOI] [PubMed] [Google Scholar]

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