Abstract
When L5178Y and L1210 mouse lymphosarcoma cells were incubated with rat or beef liver arginase there was up to 100% cell destruction in 24 hours. This was reversed specifically with arginine and partially with arginino-succinic acid, citrulline and ornithine. The concentration of arginine was critical; at 8 μmol/l the cells remained viable and reversible inhibition could be shown; below this level cells died. L5178Y cells were grown in medium containing from 0 to 80 μmol/l arginine for 24 hours then transferred to fresh medium for 24 hours. Viable cell counts and mitotic indices were determined, and cells were pulsed with 3H-thymidine, 3H-uridine, 14C-leucine and 14C-arginine at various times. Thymidine uptake was affected most and preceded parallel changes in viable cell numbers. It was concluded that arginine is required by these cells even in a “resting” state and despite some evidence for their capacity to utilize precursors, the tumour cells underwent rapid and extensive destruction when available arginine was severely depleted.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BACH S. J., SWAINE D. THE EFFECT OF ARGINASE ON THE RETARDATION OF TUMOUR GROWTH. Br J Cancer. 1965 Jun;19:379–386. doi: 10.1038/bjc.1965.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burton A. F., Storr J. M., Dunn W. L. Cytolytic action of corticosteroids on thymus and lymphoma cells in vitro. Can J Biochem. 1967 Feb;45(2):289–297. doi: 10.1139/o67-032. [DOI] [PubMed] [Google Scholar]
- Freed J. J., Sorof S. Reversible inhibition of cell multiplication by a small class of liver proteins. Biochem Biophys Res Commun. 1966 Jan 4;22(1):1–5. doi: 10.1016/0006-291x(66)90592-4. [DOI] [PubMed] [Google Scholar]
- HNILICA L. S., BUSCH H. Fractionation of the histones of the Walker 256 carcinosarcoma by combined chemical and chromatographic techniques. J Biol Chem. 1963 Mar;238:918–924. [PubMed] [Google Scholar]
- Holley R. W. Evidence that a rat liver "inhibitor" of the synthesis of DNA in cultured mammalian cells is arginase. Biochim Biophys Acta. 1967 Sep 26;145(2):525–527. doi: 10.1016/0005-2787(67)90076-7. [DOI] [PubMed] [Google Scholar]
- Krebs H. A., Eggleston L. V. The oxidation of pyruvate in pigeon breast muscle. Biochem J. 1940 Mar;34(3):442–459. doi: 10.1042/bj0340442. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LIEBERMAN I., OVE P. Inhibition of growth of cultured mammalian cells by liver extracts. Biochim Biophys Acta. 1960 Feb 12;38:153–153. doi: 10.1016/0006-3002(60)91208-7. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Osunkoya B. O., Adler W. H., Smith R. T. Effect of arginine deficiency on synthesis of DNA and immunoglobulin receptor of Burkitt lymphoma cells. Nature. 1970 Jul 25;227(5256):398–399. doi: 10.1038/227398a0. [DOI] [PubMed] [Google Scholar]
- Schrek R., Holcenberg J. S., Roberts J., Dolowy W. C. In vitro cytocidal effect of L-glutaminase on leukaemic lymphocytes. Nature. 1971 Jul 23;232(5308):265–265. doi: 10.1038/232265a0. [DOI] [PubMed] [Google Scholar]
- Senft A. W. Studies in arginine metabolism by schistosomes. II. Arginine depletion in mammals and snails infected with S. mansoni or S. hematobium. Comp Biochem Physiol. 1967 May;21(2):299–306. doi: 10.1016/0010-406x(67)90790-6. [DOI] [PubMed] [Google Scholar]
- Sorof S., Young E. M., McBride R. A., Coffey C. B. Size classes of soluble liver macromolecules. Arch Biochem Biophys. 1966 Jan;113(1):83–96. doi: 10.1016/0003-9861(66)90159-7. [DOI] [PubMed] [Google Scholar]
- Thomas J. W., Boldt W., Horrocks G., Low B. Lymphocyte transformation by phytohemagglutinin. I. In Hodgkin's Disease. Can Med Assoc J. 1967 Sep 30;97(14):832–836. [PMC free article] [PubMed] [Google Scholar]