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. 1981 May;43(5):696–700. doi: 10.1038/bjc.1981.101

Human monoclonal antibodies to lung-cancer antigens.

K Sikora, R Wright
PMCID: PMC2010695  PMID: 7248154

Abstract

Lymphocytes obtained from hilar and bronchial lymph nodes from 23 patients undergoing radical surgery for carcinoma of the bronchus were fused with established rat or mouse myeloma lines. 62% of the resultant hybrids were found to be secreting human Ig detected by a sensitive staphylococcal Protein A-coupled SRBC assay. Immunoglobulins synthesized by such hybrids were internally labelled with 3H-lysine and their antibody activity against a variety of membrane preparations determined. Nine monoclonal antibodies were found which bound to molecules on lung-cancer membranes and not on normal lung membranes from the same patient.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baldwin R. W. In vitro assays of cell-mediated immunity to human solid tumors: problems of quantitation, specificity, and interpretation. J Natl Cancer Inst. 1975 Oct;55(4):745–748. doi: 10.1093/jnci/55.4.745. [DOI] [PubMed] [Google Scholar]
  2. Gaffar S. A., Braatz J. A., Kortright K. H., Princler G. L., McIntire K. R. Further studies on a human lung tumor-associated antigen. Comparison of antigens from different tumors. J Biol Chem. 1979 Mar 25;254(6):2097–2102. [PubMed] [Google Scholar]
  3. Herlyn M., Steplewski Z., Herlyn D., Koprowski H. Colorectal carcinoma-specific antigen: detection by means of monoclonal antibodies. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1438–1442. doi: 10.1073/pnas.76.3.1438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hollinshead A. C., Stewart T. H., Herberman R. B. Delayed-hypersensitivity reactions to soluble membrane antigens of human malignant lung cells. J Natl Cancer Inst. 1974 Feb;52(2):327–338. doi: 10.1093/jnci/52.2.327. [DOI] [PubMed] [Google Scholar]
  5. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  6. Levy R., Dilley J., Fox R. I., Warnke R. A human thymus-leukemia antigen defined by hybridoma monoclonal antibodies. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6552–6556. doi: 10.1073/pnas.76.12.6552. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Olsson L., Kaplan H. S. Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5429–5431. doi: 10.1073/pnas.77.9.5429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Poston R. N. A buffered chromic chloride method of attaching antigens to red cells: use in haemagglutination. J Immunol Methods. 1974 May;5(1):91–96. doi: 10.1016/0022-1759(74)90050-7. [DOI] [PubMed] [Google Scholar]
  9. Reinherz E. L., Kung P. C., Goldstein G., Schlossman S. F. Separation of functional subsets of human T cells by a monoclonal antibody. Proc Natl Acad Sci U S A. 1979 Aug;76(8):4061–4065. doi: 10.1073/pnas.76.8.4061. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schultz R. M., Woods W. A., Chirigos M. A. Detection in colorectal carcinoma patients of antibody cytotoxic to established cell strains derived from carcinoma of the human colon and rectum. Int J Cancer. 1975 Jul 15;16(1):16–23. doi: 10.1002/ijc.2910160104. [DOI] [PubMed] [Google Scholar]
  11. Sikora K., Koch G., Brenner S., Lennox E. Partial purification of tumour-specific transplantation antigens from methylcholanthrene-induced murine sarcomas by immobilized lectins. Br J Cancer. 1979 Dec;40(6):831–838. doi: 10.1038/bjc.1979.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Simrell C. R., Klein P. A. Antibody responses of tumor-bearing mice to their own tumors captured and perpetuated as hybridomas. J Immunol. 1979 Nov;123(5):2386–2394. [PubMed] [Google Scholar]
  13. Takasugi M., Mickey M. R., Terasaki P. I. Studies on specificity of cell-mediated immunity to human tumors. J Natl Cancer Inst. 1974 Dec;53(6):1527–1538. [PubMed] [Google Scholar]
  14. Yeh M. Y., Hellström I., Brown J. P., Warner G. A., Hansen J. A., Hellström K. E. Cell surface antigens of human melanoma identified by monoclonal antibody. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2927–2931. doi: 10.1073/pnas.76.6.2927. [DOI] [PMC free article] [PubMed] [Google Scholar]

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