Abstract
Rat monoclonal antibodies were prepared by immunising rats with human colorectal carcinoma cell membranes and fusing splenic lymphocytes with a rat myeloma. Hybridoma supernatants were screened by binding assays on membranes prepared from colorectal carcinoma tissue. One hybridoma supernatant, containing a monoclonal antibody with high binding activity on malignant compared to normal colon sections, was grown in large quantities in serum-free medium. After ammonium sulphate precipitation the antibody was purified by ion-exchange chromatography and labelled with 131I. Radiolabelled antibody was administered i.v. to 27 patients with colonic and other tumours. Scintigrams were obtained at 48 h. Computerised subtraction of the blood pool image revealed localised areas of uptake corresponding with areas of known disease in 13/16 patients with colorectal carcinoma and 3/4 patients with breast cancer.
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- Dykes P. W., Hine K. R., Bradwell A. R., Blackburn J. C., Reeder T. A., Drolc Z., Booth S. N. Localisation of tumour deposits by external scanning after injection of radiolabelled anti-carcinoembryonic antigen. Br Med J. 1980 Jan 26;280(6209):220–222. doi: 10.1136/bmj.280.6209.220. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Galfrè G., Milstein C., Wright B. Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG. Nature. 1979 Jan 11;277(5692):131–133. doi: 10.1038/277131a0. [DOI] [PubMed] [Google Scholar]
- Goldenberg D. M., Kim E. E., DeLand F. H., Bennett S., Primus F. J. Radioimmunodetection of cancer with radioactive antibodies to carcinoembryonic antigen. Cancer Res. 1980 Aug;40(8 Pt 2):2984–2992. [PubMed] [Google Scholar]
- Hales A. A procedure for the fusion of cells in suspension by means of polyethylene glycol. Somatic Cell Genet. 1977 Mar;3(2):227–230. doi: 10.1007/BF01551817. [DOI] [PubMed] [Google Scholar]
- Levine G., Ballou B., Reiland J., Solter D., Gumerman L., Hakala T. Localization of I-131-labeled tumor-specific monoclonal antibody in the tumor-bearing BALB/c mouse. J Nucl Med. 1980 Jun;21(6):570–573. [PubMed] [Google Scholar]
- Miller R. A., Ruddle F. H. Pluripotent teratocarcinoma-thymus somatic cell hybrids. Cell. 1976 Sep;9(1):45–55. doi: 10.1016/0092-8674(76)90051-9. [DOI] [PubMed] [Google Scholar]
- Moshakis V., McIlhinney R., Raghavan D., Neville A. M. Monoclonal antibodies to detect human tumours: an experimental approach. J Clin Pathol. 1981 Mar;34(3):314–319. doi: 10.1136/jcp.34.3.314. [DOI] [PMC free article] [PubMed] [Google Scholar]