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. 1983 Feb;47(2):245–252. doi: 10.1038/bjc.1983.32

In vitro thymidine labelling of human pulmonary neoplasms.

K M Kerr, A M Robertson, D Lamb
PMCID: PMC2011269  PMID: 6824569

Abstract

The in vitro thymidine labelling indices (TLI) of 58 human lung tumours were assessed using autoradiography. The labelling technique involved incubation of 1 mm3 tumour fragments with 3H-thymidine (5 muCi ml-1) under conditions of hyperbaric oxygenation at a pressure of 3 atmospheres. Only a rim of labelling was achieved along the edges of fragments and the depth of this rim varied from tumour to tumour. A technique for counting TLIs was therefore devised to take this into account. In general, those tumours showing low TLI values of less than 5.0% showed a greater depth of labelling. The common malignant tumours of the bronchus showed a wide range of values (2.2-30.4%) though the adenocarcinomata had a lower average value than the other groups. With the squamous carcinomata a relationship with differentiation was shown. The mean value for small cell carcinomata (16.9%)--a highly aggressive tumour--was no higher than for the other groups. The low grade malignant tumours showed TLIs of less than 3.0% and these values correlate with their less aggressive clinical behaviour. Labelling of stromal cells and inflammatory cells varied greatly from tumour to tumour; however, no correlation was found with the TLIs of tumour cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chavaudra N., Richard J. M., Malaise E. P. Labelling index of human squamous cell carcinomas. Comparison of in vivo and in vitro labelling methods. Cell Tissue Kinet. 1979 Mar;12(2):145–152. doi: 10.1111/j.1365-2184.1979.tb00121.x. [DOI] [PubMed] [Google Scholar]
  2. Coons H., Norman A., Nahum A. M. In vitro measurements of human tumor growth. Cancer. 1966 Sep;19(9):1200–1204. doi: 10.1002/1097-0142(196609)19:9<1200::aid-cncr2820190904>3.0.co;2-c. [DOI] [PubMed] [Google Scholar]
  3. Denekamp J., Kallman R. F. In vitro and in vivo labelling of animal tumours with tritiated thymidine. Cell Tissue Kinet. 1973 May;6(3):217–227. doi: 10.1111/j.1365-2184.1973.tb01611.x. [DOI] [PubMed] [Google Scholar]
  4. Fabrikant J. I., Wisseman C. L., 3rd, Vitak M. J. The kinetics of cellular proliferation in normal and malignant tissues. II. An in vitro method for incorporation of tritiated thymidine in human tissues. Radiology. 1969 May;92(6):1309–1320. doi: 10.1148/92.6.1309. [DOI] [PubMed] [Google Scholar]
  5. Hainau B., Dombernowsky P., Hansen H. H., Borgeskov S. Cell proliferation and histologic classification of bronchogenic carcinoma. J Natl Cancer Inst. 1977 Oct;59(4):1113–1118. doi: 10.1093/jnci/59.4.1113. [DOI] [PubMed] [Google Scholar]
  6. JOHNSON H. A., BOND V. P. A method of labeling tissues with tritiated thymidine in vitro and its use in comparing rates of cell proliferation in duct epithelium, fibroadenoma, and carcinoma of human breast. Cancer. 1961 May-Jun;14:639–643. doi: 10.1002/1097-0142(199005/06)14:3<639::aid-cncr2820140327>3.0.co;2-c. [DOI] [PubMed] [Google Scholar]
  7. Lieb L. M., Lisco H. In vitro uptake of tritiated thymidine by carcinoma of the human colon. Cancer Res. 1966 Apr;26(4):733–740. [PubMed] [Google Scholar]
  8. Malaise E. P., Chavaudra N., Tubiana M. The relationship between growth rate, labelling index and histological type of human solid tumours. Eur J Cancer. 1973 Apr;9(4):305–312. doi: 10.1016/0014-2964(73)90099-6. [DOI] [PubMed] [Google Scholar]
  9. Meyer J. S., Bauer W. C. In vitro determination of tritiated thymidine labeling index (LI). Evaluation of a method utilizing hyperbaric oxygen and observations on the LI of human mammary carcinoma. Cancer. 1975 Oct;36(4):1374–1380. doi: 10.1002/1097-0142(197510)36:4<1374::aid-cncr2820360428>3.0.co;2-s. [DOI] [PubMed] [Google Scholar]
  10. Meyer J. S., Connor R. E. In vitro labeling of solid tissues with tritiated thymidine for autoradiographic detection of S-phase nuclei. Stain Technol. 1977 Jul;52(4):185–195. doi: 10.3109/10520297709116774. [DOI] [PubMed] [Google Scholar]
  11. Muggia F. M. Correlation of histologic types with cell kinetic studies in lung cancer. Cancer Chemother Rep 3. 1973 Mar;4(2):69–71. [PubMed] [Google Scholar]
  12. Nordenskjöld B., Zetterberg A., Löwhagen T. Measurement of DNA synthesis by 3H-thymidine incorporation into needle aspirates from human tumors. Acta Cytol. 1974 May-Jun;18(3):215–221. [PubMed] [Google Scholar]
  13. Sklarew R. J., Hoffman J., Post J. A rapid in vitro method for measuring cell proliferation in human breast cancer. Cancer. 1977 Nov;40(5):2299–2302. doi: 10.1002/1097-0142(197711)40:5<2299::aid-cncr2820400542>3.0.co;2-#. [DOI] [PubMed] [Google Scholar]
  14. Sky-Peck H. H. Effects of chemotherapy on the incorporation of 3 H-thymidine into DNA of human neoplastic tissue. Natl Cancer Inst Monogr. 1971 Dec;34:197–203. [PubMed] [Google Scholar]
  15. Steel G. G., Bensted J. P. In vitro studies of cell proliferation in tumours. I. Critical appraisal of methods and theoretical considerations. Eur J Cancer. 1965 Nov;1(3):275–279. doi: 10.1016/0014-2964(65)90060-5. [DOI] [PubMed] [Google Scholar]
  16. Straus M. J., Moran R. E. Cell cycle parameters in human solid tumors. Cancer. 1977 Oct;40(4):1453–1461. doi: 10.1002/1097-0142(197710)40:4<1453::aid-cncr2820400416>3.0.co;2-e. [DOI] [PubMed] [Google Scholar]
  17. TITUS J. L., SHORTER R. G. LABELING OF HUMAN TUMORS WITH TRITIATED THYMIDINE. Arch Pathol. 1965 Mar;79:324–328. [PubMed] [Google Scholar]
  18. Terz J. J., Curutchet H. P., Lawrence W., Jr Analysis of the cell kinetics of human solid tumors. Cancer. 1971 Nov;28(5):1100–1110. doi: 10.1002/1097-0142(1971)28:5<1100::aid-cncr2820280503>3.0.co;2-1. [DOI] [PubMed] [Google Scholar]
  19. Tubiana M. The kinetics of tumour cell proliferation and radiotherapy. Br J Radiol. 1971 May;44(521):325–347. doi: 10.1259/0007-1285-44-521-325. [DOI] [PubMed] [Google Scholar]
  20. WOLBERG W. H., BROWN R. R. Autoradiographic studies of in vitro incorporation of uridine and thymidine by human tumor tissue. Cancer Res. 1962 Oct;22:1113–1119. [PubMed] [Google Scholar]

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