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. 1983 Dec;48(6):763–775. doi: 10.1038/bjc.1983.265

Routine immunofluorescent and histochemical analysis of bone marrow involvement of lymphoma/leukaemia: the use of cryostat sections.

M Chilosi, G Pizzolo, L Fiore-Donati, M Bofill, G Janossy
PMCID: PMC2011562  PMID: 6360192

Abstract

Enzyme histochemical and immunohistological (immuno-fluorescence and -peroxidase) techniques have been routinely used for investigating over 70 normal and pathological bone marrow samples. This recently standardized diagnostic procedure is very quick and can be performed in a few hours. In 6 cases the clinical diagnosis of leukaemia/lymphoma has become apparent only after the immunohistological analysis of the bone marrow. In 6 other cases the information about the staging of B cell malignancies was superior in the frozen biopsies to the paraffin embedded preparations. Amongst many other features the monoclonality of B CLL/lymphomas, the special features of B CLL infiltrates (RFA-1+, Leu-1+, HLA-DR+, SmIg+), follicular lymphoma deposits (containing follicular dendritic cells) and non-T, non-B acute lymphoblastic leukaemic blasts (terminal transferase+, HLA-DR+) as well as the sometimes conspicuous presence of infiltrating normal T cells could be clearly and reproducibly demonstrated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beckstead J. H., Bainton D. F. Enzyme histochemistry on bone marrow biopsies: reactions useful in the differential diagnosis of leukemia and lymphoma applied to 2-micron plastic sections. Blood. 1980 Mar;55(3):386–394. [PubMed] [Google Scholar]
  2. Beverley P. C., Callard R. E. Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. Eur J Immunol. 1981 Apr;11(4):329–334. doi: 10.1002/eji.1830110412. [DOI] [PubMed] [Google Scholar]
  3. Bodger M. P., Francis G. E., Delia D., Granger S. M., Janossy G. A monoclonal antibody specific for immature human hemopoietic cells and T lineage cells. J Immunol. 1981 Dec;127(6):2269–2274. [PubMed] [Google Scholar]
  4. Bollum F. J. Antibody to terminal deoxynucleotidyl transferase. Proc Natl Acad Sci U S A. 1975 Oct;72(10):4119–4122. doi: 10.1073/pnas.72.10.4119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brynes R. K., McKenna R. W., Sundberg R. D. Bone marrow aspiration and trephine biopsy. An approach to a thorough study. Am J Clin Pathol. 1978 Nov;70(5):753–759. doi: 10.1093/ajcp/70.5.753. [DOI] [PubMed] [Google Scholar]
  6. Burkhardt R., Frisch B., Bartl R. Bone biopsy in haematological disorders. J Clin Pathol. 1982 Mar;35(3):257–284. doi: 10.1136/jcp.35.3.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Caligaris-Cappio F., Gobbi M., Bofill M., Janossy G. Infrequent normal B lymphocytes express features of B-chronic lymphocytic leukemia. J Exp Med. 1982 Feb 1;155(2):623–628. doi: 10.1084/jem.155.2.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Castro-Malaspina H., Gay R. E., Resnick G., Kapoor N., Meyers P., Chiarieri D., McKenzie S., Broxmeyer H. E., Moore M. A. Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny. Blood. 1980 Aug;56(2):289–301. [PubMed] [Google Scholar]
  9. Chilosi M., Pizzolo G., Janossy G., Bofill M., Fiore-Donati L. Enzyme histochemical analysis on cryostat sections of human bone marrow. J Clin Pathol. 1982 Nov;35(11):1220–1226. doi: 10.1136/jcp.35.11.1220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Chilosi M., Pizzolo G., Menestrina F., Iannucci A. M., Bonetti F., Fiore-Donati L. Dipeptidyl(amino)peptidase IV (DAP-IV) histochemistry on normal and pathologic lymphoid tissues. Am J Clin Pathol. 1982 Jun;77(6):714–719. doi: 10.1093/ajcp/77.6.714. [DOI] [PubMed] [Google Scholar]
  11. Chilosi M., Pizzolo G., Menestrina F., Iannucci A. M., Bonetti F., Fiore-Donati L. Enzyme histochemistry on normal and pathologic paraffin-embedded lymphoid tissues. Am J Clin Pathol. 1981 Dec;76(6):729–736. doi: 10.1093/ajcp/76.6.729. [DOI] [PubMed] [Google Scholar]
  12. Chilosi M., Pizzolo G., Vincenzi C. Haematoxylin counterstaining of immunofluorescence preparations. J Clin Pathol. 1983 Jan;36(1):114–115. doi: 10.1136/jcp.36.1.114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Decker R. S., Decker M. L., Poole A. R. The distribution of lysosomal cathepsin D in cardiac myocytes. J Histochem Cytochem. 1980 Mar;28(3):231–237. doi: 10.1177/28.3.6986433. [DOI] [PubMed] [Google Scholar]
  14. Dick F., Bloomfield C. D., Brunning R. D. Incidence cytology, and histopathology of non-Hodgkin's lymphomas in the bone marrow. Cancer. 1974 May;33(5):1382–1398. doi: 10.1002/1097-0142(197405)33:5<1382::aid-cncr2820330525>3.0.co;2-2. [DOI] [PubMed] [Google Scholar]
  15. Gatter K. C., Abdulaziz Z., Beverley P., Corvalan J. R., Ford C., Lane E. B., Mota M., Nash J. R., Pulford K., Stein H. Use of monoclonal antibodies for the histopathological diagnosis of human malignancy. J Clin Pathol. 1982 Nov;35(11):1253–1267. doi: 10.1136/jcp.35.11.1253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Gerdes J., Naiem M., Mason D. Y., Stein H. Human complement (C3b) receptors defined by a mouse monoclonal antibody. Immunology. 1982 Apr;45(4):645–653. [PMC free article] [PubMed] [Google Scholar]
  17. Gerdes J., Schwab U., Lemke H., Stein H. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 1983 Jan 15;31(1):13–20. doi: 10.1002/ijc.2910310104. [DOI] [PubMed] [Google Scholar]
  18. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  19. Hanker J. S., Ambrose W. W., James C. J., Mandelkorn J., Yates P. E., Gall S. A., Bossen E. H., Fay J. W., Laszlo J., Moore J. O. Facilitated light microscopic cytochemical diagnosis of acute myelogenous leukemia. Cancer Res. 1979 May;39(5):1635–1639. [PubMed] [Google Scholar]
  20. Janossy G., Bollum F. J., Bradstock K. F., McMichael A., Rapson N., Greaves M. F. Terminal transferase-positive human bone marrow cells exhibit the antigenic phenotype of common acute lymphoblastic leukemia. J Immunol. 1979 Oct;123(4):1525–1529. [PubMed] [Google Scholar]
  21. Janossy G., Thomas J. A., Pizzolo G., Granger S. M., McLaughlin J., Habeshaw J. A., Stansfeld A. G., Sloane J. Immuno-histological diagnosis of lymphoproliferative diseases by selected combinations of antisera and monoclonal antibodies. Br J Cancer. 1980 Aug;42(2):224–242. doi: 10.1038/bjc.1980.222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Johnson G. D., Nogueira Araujo G. M. A simple method of reducing the fading of immunofluorescence during microscopy. J Immunol Methods. 1981;43(3):349–350. doi: 10.1016/0022-1759(81)90183-6. [DOI] [PubMed] [Google Scholar]
  23. Martin P. J., Hansen J. A., Siadak A. W., Nowinski R. C. Monoclonal antibodies recognizing normal human T lymphocytes and malignant human B lymphocytes: a comparative study. J Immunol. 1981 Nov;127(5):1920–1923. [PubMed] [Google Scholar]
  24. Oi V. T., Glazer A. N., Stryer L. Fluorescent phycobiliprotein conjugates for analyses of cells and molecules. J Cell Biol. 1982 Jun;93(3):981–986. doi: 10.1083/jcb.93.3.981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Pizzolo G., Sloane J., Beverley P., Thomas J. A., Bradstock K. F., Mattingly S., Janossy G. Differential diagnosis of malignant lymphoma and nonlymphoid tumors using monoclonal anti-leucocyte antibody. Cancer. 1980 Dec 15;46(12):2640–2647. doi: 10.1002/1097-0142(19801215)46:12<2640::aid-cncr2820461218>3.0.co;2-s. [DOI] [PubMed] [Google Scholar]
  26. Platsoucas C. D., Galinski M., Kempin S., Reich L., Clarkson B., Good R. A. Abnormal T lymphocyte subpopulations in patients with B cell chronic lymphocytic leukemia: an analysis by monoclonal antibodies. J Immunol. 1982 Nov;129(5):2305–2312. [PubMed] [Google Scholar]
  27. Poppema S., Bhan A. K., Reinherz E. L., Posner M. R., Schlossman S. F. In situ immunologic characterization of cellular constituents in lymph nodes and spleens involved by Hodgkin's disease. Blood. 1982 Feb;59(2):226–232. [PubMed] [Google Scholar]
  28. Poppema S., Elema J. D., Halie M. R. Alkaline phosphatase positive lymphomas: a morphologic, immunologic, and enzymehistochemical study. Cancer. 1981 Mar 15;47(6):1303–1312. doi: 10.1002/1097-0142(19810315)47:6<1303::aid-cncr2820470612>3.0.co;2-s. [DOI] [PubMed] [Google Scholar]
  29. Reincke U., Hsieh P., Mauch P., Hellman S., Chen L. B. Cell types associated with fibronectin in long-term mouse bone marrow cultures. J Histochem Cytochem. 1982 Mar;30(3):235–244. doi: 10.1177/30.3.7061825. [DOI] [PubMed] [Google Scholar]
  30. Royston I., Majda J. A., Baird S. M., Meserve B. L., Griffiths J. C. Human T cell antigens defined by monoclonal antibodies: the 65,000-dalton antigen of T cells (T65) is also found on chronic lymphocytic leukemia cells bearing surface immunoglobulin. J Immunol. 1980 Aug;125(2):725–731. [PubMed] [Google Scholar]
  31. Savage R. A., Hoffman G. C., Shaker K. Diagnostic problems involved in detection of metastatic neoplasms by bone-marrow aspirate compared with needle biopsy. Am J Clin Pathol. 1978 Oct;70(4):623–627. doi: 10.1093/ajcp/70.4.623. [DOI] [PubMed] [Google Scholar]
  32. Stein H., Bonk A., Tolksdorf G., Lennert K., Rodt H., Gerdes J. Immunohistologic analysis of the organization of normal lymphoid tissue and non-Hodgkin's lymphomas. J Histochem Cytochem. 1980 Aug;28(8):746–760. doi: 10.1177/28.8.7003001. [DOI] [PubMed] [Google Scholar]
  33. Stein H., Gerdes J., Mason D. Y. The normal and malignant germinal centre. Clin Haematol. 1982 Oct;11(3):531–559. [PubMed] [Google Scholar]
  34. Straus W. Peroxidase procedures. Technical problems encountered during their application. J Histochem Cytochem. 1979 Oct;27(10):1349–1351. doi: 10.1177/27.10.92491. [DOI] [PubMed] [Google Scholar]
  35. Taylor C. R., Kledzik G. Immunohistologic techniques in surgical pathology - a spectrum of "new" special stains. Hum Pathol. 1981 Jul;12(7):590–596. doi: 10.1016/s0046-8177(81)80041-x. [DOI] [PubMed] [Google Scholar]
  36. Thomas J. A., Janossy G., Eden O. B., Bollum F. J. Nuclear terminal deoxynucleotidyl transferase in leukaemic infiltrates of testicular tissue. Br J Cancer. 1982 May;45(5):709–717. doi: 10.1038/bjc.1982.112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Trejdosiewicz L. K., Smolira M. A., Hodges G. M., Goodman S. L., Livingston D. C. Cell surface distribution of fibronectin in cultures of fibroblasts and bladder derived epithelium: SEM-immunogold localization compared to immunoperoxidase and immunofluorescence.. J Microsc. 1981 Aug;123(Pt 2):227–236. doi: 10.1111/j.1365-2818.1981.tb01297.x. [DOI] [PubMed] [Google Scholar]
  38. Westen H., Bainton D. F. Association of alkaline-phosphatase-positive reticulum cells in bone marrow with granulocytic precursors. J Exp Med. 1979 Oct 1;150(4):919–937. doi: 10.1084/jem.150.4.919. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Wood G. S., Warnke R. A. The immunologic phenotyping of bone marrow biopsies and aspirates: frozen section techniques. Blood. 1982 May;59(5):913–922. [PubMed] [Google Scholar]

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