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. 1994 Jan;60(1):323–327. doi: 10.1128/aem.60.1.323-327.1994

A method for detection of aromatic metabolites at very low concentrations: application to detection of metabolites of anaerobic toluene degradation.

E A Edwards 1, A M Edwards 1, D Grbić-Galić 1
PMCID: PMC201306  PMID: 8117085

Abstract

Difficulties inherent in working with anaerobic microorganisms and mixed cultures have hampered efforts to detect and identify metabolites of anaerobic degradation of monoaromatic compounds. Isotope-trapping experiments and analysis using a high-performance liquid chromatograph equipped with a flow-through radioactivity detector were used to detect very low concentrations of metabolites. Data obtained by this method suggest that toluene was degraded via methyl hydroxylation by a mixed methanogenic culture.

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Selected References

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