Abstract
A panel of 11 monoclonal antibodies raised against vegetative cells of Rhizobium leguminosarum biovar trifolii or Rhizobium meliloti was tested by enzyme-linked immunosorbent assay for reactivity with 47 strains of R. leguminosarum biovar trifolii and 60 strains of R. meliloti. The goal of the study was to define the degree of specificity associated with each antibody and to gain an understanding of the amount of antigenic diversity found among the strains and between the species. Each antibody was tested against each Rhizobium strain in four forms: washed steamed cells, washed unsteamed cells, cell-free culture broth, and nodule squash material. Each antibody showed a different pattern of reactivity among the 107 strains. One of each of the antibodies developed against R. meliloti and R. leguminosarum biovar trifolii reacted in a highly specific manner with cells or antigen from the immunogenic strain only. Nine of the antibodies recognized secreted as well as cellular antigen from many of the strains. Analysis of patterns of reactivity between the 107 strains and the 11 antibodies separated the strains into 28 groups of which 12 were represented by one strain only.
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- Ayanaba A., Weiland K. D., Zablotowicz R. M. Evaluation of Diverse Antisera, Conjugates, and Support Media for Detecting Bradyrhizobium japonicum by Indirect Enzyme-Linked Immunosorbent Assay. Appl Environ Microbiol. 1986 Nov;52(5):1132–1138. doi: 10.1128/aem.52.5.1132-1138.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- George M. L., Robert F. M. Competition among Rhizobium leguminosarum bv. phaseoli strains for nodulation of common bean. Can J Microbiol. 1992 Feb;38(2):157–160. doi: 10.1139/m92-026. [DOI] [PubMed] [Google Scholar]
- Johansen E., Finan T. M., Gefter M. L., Signer E. R. Monoclonal antibodies to Rhizobium meliloti and surface mutants insensitive to them. J Bacteriol. 1984 Oct;160(1):454–457. doi: 10.1128/jb.160.1.454-457.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kannenberg E. L., Brewin N. J. Expression of a cell surface antigen from Rhizobium leguminosarum 3841 is regulated by oxygen and pH. J Bacteriol. 1989 Sep;171(9):4543–4548. doi: 10.1128/jb.171.9.4543-4548.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kishinevsky B., Bar-Joseph M. Rhizobium strain identification in Arachis hypogaea nodules by enzyme-linked immunosorbent assay (ELISA). Can J Microbiol. 1978 Dec;24(12):1537–1543. doi: 10.1139/m78-245. [DOI] [PubMed] [Google Scholar]
- Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
- Schmidt E. L., Bakole R. O., Bohlool B. B. Fluorescent-antibody approach to study of rhizobia in soil. J Bacteriol. 1968 Jun;95(6):1987–1992. doi: 10.1128/jb.95.6.1987-1992.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wright S. F., Foster J. G., Bennett O. L. Production and Use of Monoclonal Antibodies for Identification of Strains of Rhizobium trifolii. Appl Environ Microbiol. 1986 Jul;52(1):119–123. doi: 10.1128/aem.52.1.119-123.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wright S. F. Production and epitope analysis of monoclonal antibodies against a Rhizobium leguminosarum biovar trifolii strain. Appl Environ Microbiol. 1990 Jul;56(7):2262–2264. doi: 10.1128/aem.56.7.2262-2264.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Maagd R., de Rijk R., Mulders I. H., Lugtenberg B. J. Immunological characterization of Rhizobium leguminosarum outer membrane antigens by use of polyclonal and monoclonal antibodies. J Bacteriol. 1989 Feb;171(2):1136–1142. doi: 10.1128/jb.171.2.1136-1142.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]