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. 1994 Mar;60(3):1018–1020. doi: 10.1128/aem.60.3.1018-1020.1994

Inactivation of ccmO in Synechococcus sp. Strain PCC 7942 Results in a Mutant Requiring High Levels of CO2

E Marco 1,†, I Martinez 1,†, M Ronen-Tarazi 1, M I Orus 1,†, A Kaplan 1,*
PMCID: PMC201426  PMID: 16349207

Abstract

Inactivation of ccmO in Synechococcus sp. strain PCC 7942 resulted in a mutant which possesses aberrant carboxysomes and a normal inorganic carbon uptake capability but a reduced ability to photosynthetically utilize the internal inorganic carbon pool. Consequently, it exhibits low apparent photosynthetic affinity for extracellular inorganic carbon and demands high levels of CO2 for growth.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bédu S., Peltier G., Sarrey F., Joset F. Properties of a Mutant from Synechocystis PCC6803 Resistant to Acetazolamide, an Inhibitor of Carbonic Anhydrase. Plant Physiol. 1990 Aug;93(4):1312–1315. doi: 10.1104/pp.93.4.1312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Friedberg D., Kaplan A., Ariel R., Kessel M., Seijffers J. The 5'-flanking region of the gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase is crucial for growth of the cyanobacterium Synechococcus sp. strain PCC 7942 at the level of CO2 in air. J Bacteriol. 1989 Nov;171(11):6069–6076. doi: 10.1128/jb.171.11.6069-6076.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kaplan A., Schwarz R., Lieman-Hurwitz J., Reinhold L. Physiological and molecular aspects of the inorganic carbon-concentrating mechanism in cyanobacteria. Plant Physiol. 1991 Nov;97(3):851–855. doi: 10.1104/pp.97.3.851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Marco E., Ohad N., Schwarz R., Lieman-Hurwitz J., Gabay C., Kaplan A. High CO2 concentration alleviates the block in photosynthetic electron transport in an ndhB-inactivated mutant of Synechococcus sp. PCC 7942. Plant Physiol. 1993 Mar;101(3):1047–1053. doi: 10.1104/pp.101.3.1047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Marcus Y., Schwarz R., Friedberg D., Kaplan A. High CO(2) Requiring Mutant of Anacystis nidulans R(2). Plant Physiol. 1986 Oct;82(2):610–612. doi: 10.1104/pp.82.2.610. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ogawa T. A gene homologous to the subunit-2 gene of NADH dehydrogenase is essential to inorganic carbon transport of Synechocystis PCC6803. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4275–4279. doi: 10.1073/pnas.88.10.4275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Price G. D., Howitt S. M., Harrison K., Badger M. R. Analysis of a genomic DNA region from the cyanobacterium Synechococcus sp. strain PCC7942 involved in carboxysome assembly and function. J Bacteriol. 1993 May;175(10):2871–2879. doi: 10.1128/jb.175.10.2871-2879.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

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