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. 1994 May;60(5):1532–1537. doi: 10.1128/aem.60.5.1532-1537.1994

Limited Genetic Diversity in the Endophytic Sugarcane Bacterium Acetobacter diazotrophicus

Jesus Caballero-Mellado 1, Esperanza Martinez-Romero 1,*
PMCID: PMC201513  PMID: 16349254

Abstract

Acetobacter diazotrophicus isolates that originated from different sugarcane cultivars growing in diverse geographic regions of Mexico and Brazil were shown to have limited genetic diversity. Measurements of polymorphism in the electrophoretic mobilities of metabolic enzymes revealed that the mean genetic diversity per enzyme locus (among the four electrophoretic types distinguished) was 0.064. The results of the genetic analysis indicate that the genetic structure of A. diazotrophicus is clonal, with one largely predominant clone. Plasmids were present in 20 of 24 isolates, and the molecular sizes of the plasmids ranged from 2.0 to 170 kb. Two plasmids (a 20- to 24-kb plasmid detected in all 20 plasmid-containing isolates and a 170-kb plasmid observed in 14 isolates) were highly conserved among the isolates examined. Regardless of the presence of plasmids, all of the isolates shared a common pattern of nif structural gene organization on the chromosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashbolt N. J., Inkerman P. A. Acetic Acid Bacterial Biota of the Pink Sugar Cane Mealybug, Saccharococcus sacchari, and Its Environs. Appl Environ Microbiol. 1990 Mar;56(3):707–712. doi: 10.1128/aem.56.3.707-712.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bender C. L., Cooksey D. A. Indigenous plasmids in Pseudomonas syringae pv. tomato: conjugative transfer and role in copper resistance. J Bacteriol. 1986 Feb;165(2):534–541. doi: 10.1128/jb.165.2.534-541.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coplin D. L., Rowan R. G., Chisholm D. A., Whitmoyer R. E. Characterization of plasmids in Erwinia stewartii. Appl Environ Microbiol. 1981 Oct;42(4):599–604. doi: 10.1128/aem.42.4.599-604.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fang H., Lin R. J., Gennis R. B. Location of heme axial ligands in the cytochrome d terminal oxidase complex of Escherichia coli determined by site-directed mutagenesis. J Biol Chem. 1989 May 15;264(14):8026–8032. [PubMed] [Google Scholar]
  5. Figurski D. H., Helinski D. R. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648–1652. doi: 10.1073/pnas.76.4.1648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Flores M., González V., Pardo M. A., Leija A., Martínez E., Romero D., Piñero D., Dávila G., Palacios R. Genomic instability in Rhizobium phaseoli. J Bacteriol. 1988 Mar;170(3):1191–1196. doi: 10.1128/jb.170.3.1191-1196.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Magariños B., Romalde J. L., Bandín I., Fouz B., Toranzo A. E. Phenotypic, antigenic, and molecular characterization of Pasteurella piscicida strains isolated from fish. Appl Environ Microbiol. 1992 Oct;58(10):3316–3322. doi: 10.1128/aem.58.10.3316-3322.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Martínez-Romero E., Segovia L., Mercante F. M., Franco A. A., Graham P., Pardo M. A. Rhizobium tropici, a novel species nodulating Phaseolus vulgaris L. beans and Leucaena sp. trees. Int J Syst Bacteriol. 1991 Jul;41(3):417–426. doi: 10.1099/00207713-41-3-417. [DOI] [PubMed] [Google Scholar]
  9. Mavingui P., Laguerre G., Berge O., Heulin T. Genetic and Phenotypic Diversity of Bacillus polymyxa in Soil and in the Wheat Rhizosphere. Appl Environ Microbiol. 1992 Jun;58(6):1894–1903. doi: 10.1128/aem.58.6.1894-1903.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McArthur J. V., Kovacic D. A., Smith M. H. Genetic diversity in natural populations of a soil bacterium across a landscape gradient. Proc Natl Acad Sci U S A. 1988 Dec;85(24):9621–9624. doi: 10.1073/pnas.85.24.9621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Musser J. M., Granoff D. M., Pattison P. E., Selander R. K. A population genetic framework for the study of invasive diseases caused by serotype b strains of Haemophilus influenzae. Proc Natl Acad Sci U S A. 1985 Aug;82(15):5078–5082. doi: 10.1073/pnas.82.15.5078. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nester E. W., Kosuge T. Plasmids specifying plant hyperplasias. Annu Rev Microbiol. 1981;35:531–565. doi: 10.1146/annurev.mi.35.100181.002531. [DOI] [PubMed] [Google Scholar]
  13. Noel K. D., Sanchez A., Fernandez L., Leemans J., Cevallos M. A. Rhizobium phaseoli symbiotic mutants with transposon Tn5 insertions. J Bacteriol. 1984 Apr;158(1):148–155. doi: 10.1128/jb.158.1.148-155.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pinero D., Martinez E., Selander R. K. Genetic diversity and relationships among isolates of Rhizobium leguminosarum biovar phaseoli. Appl Environ Microbiol. 1988 Nov;54(11):2825–2832. doi: 10.1128/aem.54.11.2825-2832.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Plazinski J., Dart P. J., Rolfe B. G. Plasmid visualization and nif gene location in nitrogen-fixing Azospirillum strains. J Bacteriol. 1983 Sep;155(3):1429–1433. doi: 10.1128/jb.155.3.1429-1433.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Quinto C., De La Vega H., Flores M., Leemans J., Cevallos M. A., Pardo M. A., Azpiroz R., De Lourdes Girard M., Calva E., Palacios R. Nitrogenase reductase: A functional multigene family in Rhizobium phaseoli. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1170–1174. doi: 10.1073/pnas.82.4.1170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rosenberg C., Boistard P., Dénarié J., Casse-Delbart F. Genes controlling early and late functions in symbiosis are located on a megaplasmid in Rhizobium meliloti. Mol Gen Genet. 1981;184(2):326–333. doi: 10.1007/BF00272926. [DOI] [PubMed] [Google Scholar]
  18. Schill W. B., Phelps S. R., Pyle S. W. Multilocus Electrophoretic Assessment of the Genetic Structure and Diversity of Yersinia ruckeri. Appl Environ Microbiol. 1984 Nov;48(5):975–979. doi: 10.1128/aem.48.5.975-979.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schmidt E. L., Zidwick M. J., Abebe H. M. Bradyrhizobium japonicum Serocluster 123 and Diversity among Member Isolates. Appl Environ Microbiol. 1986 Jun;51(6):1212–1215. doi: 10.1128/aem.51.6.1212-1215.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Segovia L., Young J. P., Martínez-Romero E. Reclassification of American Rhizobium leguminosarum biovar phaseoli type I strains as Rhizobium etli sp. nov. Int J Syst Bacteriol. 1993 Apr;43(2):374–377. doi: 10.1099/00207713-43-2-374. [DOI] [PubMed] [Google Scholar]
  21. Selander R. K., Beltran P., Smith N. H., Barker R. M., Crichton P. B., Old D. C., Musser J. M., Whittam T. S. Genetic population structure, clonal phylogeny, and pathogenicity of Salmonella paratyphi B. Infect Immun. 1990 Jun;58(6):1891–1901. doi: 10.1128/iai.58.6.1891-1901.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Selander R. K., Beltran P., Smith N. H., Helmuth R., Rubin F. A., Kopecko D. J., Ferris K., Tall B. D., Cravioto A., Musser J. M. Evolutionary genetic relationships of clones of Salmonella serovars that cause human typhoid and other enteric fevers. Infect Immun. 1990 Jul;58(7):2262–2275. doi: 10.1128/iai.58.7.2262-2275.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Selander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol. 1986 May;51(5):873–884. doi: 10.1128/aem.51.5.873-884.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Selander R. K., Levin B. R. Genetic diversity and structure in Escherichia coli populations. Science. 1980 Oct 31;210(4469):545–547. doi: 10.1126/science.6999623. [DOI] [PubMed] [Google Scholar]
  25. Selander R. K., McKinney R. M., Whittam T. S., Bibb W. F., Brenner D. J., Nolte F. S., Pattison P. E. Genetic structure of populations of Legionella pneumophila. J Bacteriol. 1985 Sep;163(3):1021–1037. doi: 10.1128/jb.163.3.1021-1037.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  27. Woomer P., Singleton P. W., Bohlool B. B. Ecological indicators of native rhizobia in tropical soils. Appl Environ Microbiol. 1988 May;54(5):1112–1116. doi: 10.1128/aem.54.5.1112-1116.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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