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. 1994 May;60(5):1670–1673. doi: 10.1128/aem.60.5.1670-1673.1994

Genetic analysis and overexpression of lipolytic activity in Bacillus subtilis.

V Dartois 1, J Y Coppée 1, C Colson 1, A Baulard 1
PMCID: PMC201535  PMID: 8017946

Abstract

The previously cloned Bacillus subtilis lipase gene (lip) was mapped on the chromosome and used in the construction of a B. subtilis derivative totally devoid of any lip sequence. Homologous overexpression was performed in this strain by subcloning the lip open reading frame on a multicopy plasmid under the control of a strong gram-positive promoter. A 100-fold overproducing strain was obtained, which should facilitate purification of the secreted protein. Furthermore, the delta lip strain BCL1050 constitutes an ideal host for the cloning of heterologous lipase genes.

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Selected References

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