Abstract
The defined medium A of W. R. Sistrom (W. R. Sistrom, J. Gen. Microbiol. 22:77-85, 1960) has been modified to allow the growth of Rhodospirillum rubrum in large-scale batch cultures under dark, semiaerobic conditions. The simultaneous use of two substrates, NH4-succinate (46 mM) and fructose (0.3%), which are utilized in aerobic and fermentative metabolism, respectively, leads to very high cell densities with a maximal yield of photosynthetic membranes.
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- Biedermann M., Drews G., Marx R., Schröder J. Der Einfluss des Sauerstoffpartialdruckes und der Antibiotica Actinomycin und Puromycin auf das Wachstum, die Synthese von Bacteriochlorophyll und die Thylakoidmorphogenese in Dunkelkulturen von Rhodospirillum rubrum. Arch Mikrobiol. 1967 Feb 20;56(2):133–147. [PubMed] [Google Scholar]
- HOLT S. C., MARR A. G. EFFECT OF LIGHT INTENSITY ON THE FORMATION OF INTRACYTOPLASMIC MEMBRANE IN RHODOSPIRILLUM RUBRUM. J Bacteriol. 1965 May;89:1421–1429. doi: 10.1128/jb.89.5.1421-1429.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hunter S. H. Organic Growth Essentials of the Aerobic Nonsulfur Photosynthetic Bacteria. J Bacteriol. 1946 Aug;52(2):213–221. doi: 10.1128/jb.52.2.213-221.1946. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kiley P. J., Kaplan S. Molecular genetics of photosynthetic membrane biosynthesis in Rhodobacter sphaeroides. Microbiol Rev. 1988 Mar;52(1):50–69. doi: 10.1128/mr.52.1.50-69.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ORMEROD J. G. The use of radioactive carbon dioxide in the measurement of carbon dioxide fixation in Rhodospirillum rubrum. Biochem J. 1956 Oct;64(2):373–380. doi: 10.1042/bj0640373. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schultz J. E., Weaver P. F. Fermentation and anaerobic respiration by Rhodospirillum rubrum and Rhodopseudomonas capsulata. J Bacteriol. 1982 Jan;149(1):181–190. doi: 10.1128/jb.149.1.181-190.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schön G. Substratverwertung und Bacteriochlorophyll-Synthese in anaerober Dunkelkultur von Rhodospirillum rubrum. I. Abhängigkeit der Bacteriochlorophyll-Synthese vonder Substratkonzentration und dem Elektronenacceptor. Zentralbl Bakteriol Orig A. 1972 May;220(1):380–386. [PubMed] [Google Scholar]
- Uffen R. L. Effect of low-intensity light on growth response and bacteriochlorophyll concentration in Rhodospirillum rubrum mutant C. J Bacteriol. 1973 Nov;116(2):1086–1088. doi: 10.1128/jb.116.2.1086-1088.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Uffen R. L. Growth properties of Rhodospirillum rubrum mutants and fermentation of pyruvate in anaerobic, dart conditions. J Bacteriol. 1973 Nov;116(2):874–884. doi: 10.1128/jb.116.2.874-884.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]