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. 1994 May;60(5):1701–1703. doi: 10.1128/aem.60.5.1701-1703.1994

Purification and characterization of the CelB endoglucanase from Streptomyces lividans 66 and DNA sequence of the encoding gene.

S Wittmann 1, F Shareck 1, D Kluepfel 1, R Morosoli 1
PMCID: PMC201545  PMID: 8017952

Abstract

The endoglucanase CelB isolated from culture filtrates of Streptomyces lividans IAF9 has an M(r) of 36,000. With carboxymethyl cellulose as the substrate, the Vmax and Km values are 110 IU/mg of enzyme and 1.3 mg/ml, respectively. Comparison of primary amino acid sequences classifies CelB in the H family of cellulases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arcand N., Kluepfel D., Paradis F. W., Morosoli R., Shareck F. Beta-mannanase of Streptomyces lividans 66: cloning and DNA sequence of the manA gene and characterization of the enzyme. Biochem J. 1993 Mar 15;290(Pt 3):857–863. doi: 10.1042/bj2900857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fernández-Abalos J. M., Sánchez P., Coll P. M., Villanueva J. R., Pérez P., Santamaría R. I. Cloning and nucleotide sequence of celA1, and endo-beta-1,4-glucanase-encoding gene from Streptomyces halstedii JM8. J Bacteriol. 1992 Oct;174(20):6368–6376. doi: 10.1128/jb.174.20.6368-6376.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gilkes N. R., Henrissat B., Kilburn D. G., Miller R. C., Jr, Warren R. A. Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families. Microbiol Rev. 1991 Jun;55(2):303–315. doi: 10.1128/mr.55.2.303-315.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  5. Lao G., Ghangas G. S., Jung E. D., Wilson D. B. DNA sequences of three beta-1,4-endoglucanase genes from Thermomonospora fusca. J Bacteriol. 1991 Jun;173(11):3397–3407. doi: 10.1128/jb.173.11.3397-3407.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lin E. S., Wilson D. B. Identification of a celE-binding protein and its potential role in induction of the celE gene in Thermomonospora fusca. J Bacteriol. 1988 Sep;170(9):3843–3846. doi: 10.1128/jb.170.9.3843-3846.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mondou F., Shareck F., Morosoli R., Kluepfel D. Cloning of the xylanase gene of Streptomyces lividans. Gene. 1986;49(3):323–329. doi: 10.1016/0378-1119(86)90368-9. [DOI] [PubMed] [Google Scholar]
  8. Nakai R., Horinouchi S., Beppu T. Cloning and nucleotide sequence of a cellulase gene, casA, from an alkalophilic Streptomyces strain. Gene. 1988 May 30;65(2):229–238. doi: 10.1016/0378-1119(88)90459-3. [DOI] [PubMed] [Google Scholar]
  9. Ooi T., Shinmyo A., Okada H., Murao S., Kawaguchi T., Arai M. Complete nucleotide sequence of a gene coding for Aspergillus aculeatus cellulase (FI-CMCase). Nucleic Acids Res. 1990 Oct 11;18(19):5884–5884. doi: 10.1093/nar/18.19.5884. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Shareck F., Roy C., Yaguchi M., Morosoli R., Kluepfel D. Sequences of three genes specifying xylanases in Streptomyces lividans. Gene. 1991 Oct 30;107(1):75–82. doi: 10.1016/0378-1119(91)90299-q. [DOI] [PubMed] [Google Scholar]
  11. Shen H., Schmuck M., Pilz I., Gilkes N. R., Kilburn D. G., Miller R. C., Jr, Warren R. A. Deletion of the linker connecting the catalytic and cellulose-binding domains of endoglucanase A (CenA) of Cellulomonas fimi alters its conformation and catalytic activity. J Biol Chem. 1991 Jun 15;266(17):11335–11340. [PubMed] [Google Scholar]
  12. Théberge M., Lacaze P., Shareck F., Morosoli R., Kluepfel D. Purification and characterization of an endoglucanase from Streptomyces lividans 66 and DNA sequence of the gene. Appl Environ Microbiol. 1992 Mar;58(3):815–820. doi: 10.1128/aem.58.3.815-820.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Vats-Mehta S., Bouvrette P., Shareck F., Morosoli R., Kluepfel D. Cloning of a second xylanase-encoding gene of Streptomyces lividans 66. Gene. 1990 Jan 31;86(1):119–122. doi: 10.1016/0378-1119(90)90123-9. [DOI] [PubMed] [Google Scholar]

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