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. 1994 Aug;60(8):3023–3026. doi: 10.1128/aem.60.8.3023-3026.1994

Detection of multiple virulence-associated genes of Listeria monocytogenes by PCR in artificially contaminated milk samples.

K J Cooray 1, T Nishibori 1, H Xiong 1, T Matsuyama 1, M Fujita 1, M Mitsuyama 1
PMCID: PMC201759  PMID: 8085838

Abstract

The inhibitory effect of milk in the PCR detection of Listeria monocytogenes could be overcome by washing the contaminated milk sample with phosphate-buffered saline and concentrating the bacteria to 1/10 of the original volume. In order to avoid a possible failure in the detection of virulent L. monocytogenes, a one-step procedure which enabled demonstration of three virulence-associated genes, prfA, hlyA, and plcB, simultaneously in a single PCR mixture was developed.

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Selected References

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