Abstract
Poly-β-hydroxybutyric acid (PHB) was produced from xylose and lactose by using Pseudomonas cepacia. Approximately 50% PHB (grams of PHB total/grams of biomass total) was produced. With a laser-based fluorescent probe, β-galactosidase activity was shown to be induced in P. cepacia cells grown on lactose but not in those grown on glucose or xylose. P. cepacia has the potential to produce biodegradable thermoplastics from hemicellulosic hydrolysates and cheese whey.
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Selected References
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- Anderson A. J., Dawes E. A. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol Rev. 1990 Dec;54(4):450–472. doi: 10.1128/mr.54.4.450-472.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Basile F., Hughes K. D., Wisniowski P. E., Gorenstein D. G., Lytle F. E., McCay-Buis T. S., Huber D. M., Hemming B. C. Fast and sensitive laser-based enzymatic detection of the lactose operon in microorganisms. Anal Biochem. 1993 May 15;211(1):55–60. doi: 10.1006/abio.1993.1232. [DOI] [PubMed] [Google Scholar]
- Bertrand J. L., Ramsay B. A., Ramsay J. A., Chavarie C. Biosynthesis of Poly-beta-Hydroxyalkanoates from Pentoses by Pseudomonas pseudoflava. Appl Environ Microbiol. 1990 Oct;56(10):3133–3138. doi: 10.1128/aem.56.10.3133-3138.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Comeau Y., Hall K. J., Oldham W. K. Determination of Poly-beta-Hydroxybutyrate and Poly-beta-Hydroxyvalerate in Activated Sludge by Gas-Liquid Chromatography. Appl Environ Microbiol. 1988 Sep;54(9):2325–2327. doi: 10.1128/aem.54.9.2325-2327.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ramsay B. A., Lomaliza K., Chavarie C., Dubé B., Bataille P., Ramsay J. A. Production of poly-(beta-hydroxybutyric-co-beta-hydroxyvaleric) acids. Appl Environ Microbiol. 1990 Jul;56(7):2093–2098. doi: 10.1128/aem.56.7.2093-2098.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ramsay B. A., Ramsay J. A., Cooper D. G. Production of Poly-beta-Hydroxyalkanoic Acid by Pseudomonas cepacia. Appl Environ Microbiol. 1989 Mar;55(3):584–589. doi: 10.1128/aem.55.3.584-589.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberts R. S., Hsu H. W., Lin K. D., Yang T. J. Amino acid metabolism of myeloma cells in culture. J Cell Sci. 1976 Aug;21(3):609–615. doi: 10.1242/jcs.21.3.609. [DOI] [PubMed] [Google Scholar]