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. 1994 Nov;60(11):4207–4209. doi: 10.1128/aem.60.11.4207-4209.1994

Duplication of the lantibiotic structural gene in M-type 49 group A streptococcus strains producing streptococcin A-M49.

W L Hynes 1, V L Friend 1, J J Ferretti 1
PMCID: PMC201964  PMID: 7993103

Abstract

Streptococcin A-M49 is produced by certain strains of M-type 49 group A streptococci. The structural gene for streptococcin A-M49 was cloned after hybridization with a probe containing the scnA lantibiotic structural gene. Sequence analysis revealed a duplication of the scnA gene; each gene (scnA' and scnA") encoded a 51-amino-acid prepeptide.

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Selected References

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  1. Banerjee S., Hansen J. N. Structure and expression of a gene encoding the precursor of subtilin, a small protein antibiotic. J Biol Chem. 1988 Jul 5;263(19):9508–9514. [PubMed] [Google Scholar]
  2. Buchman G. W., Banerjee S., Hansen J. N. Structure, expression, and evolution of a gene encoding the precursor of nisin, a small protein antibiotic. J Biol Chem. 1988 Nov 5;263(31):16260–16266. [PubMed] [Google Scholar]
  3. Huang T. T., Malke H., Ferretti J. J. Heterogeneity of the streptokinase gene in group A streptococci. Infect Immun. 1989 Feb;57(2):502–506. doi: 10.1128/iai.57.2.502-506.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hynes W. L., Ferretti J. J., Gilmore M. S., Segarra R. A. PCR amplification of streptococcal DNA using crude cell lysates. FEMS Microbiol Lett. 1992 Jul 1;73(1-2):139–142. doi: 10.1016/0378-1097(92)90597-h. [DOI] [PubMed] [Google Scholar]
  5. Hynes W. L., Ferretti J. J., Tagg J. R. Cloning of the gene encoding Streptococcin A-FF22, a novel lantibiotic produced by Streptococcus pyogenes, and determination of its nucleotide sequence. Appl Environ Microbiol. 1993 Jun;59(6):1969–1971. doi: 10.1128/aem.59.6.1969-1971.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kaletta C., Entian K. D., Jung G. Prepeptide sequence of cinnamycin (Ro 09-0198): the first structural gene of a duramycin-type lantibiotic. Eur J Biochem. 1991 Jul 15;199(2):411–415. doi: 10.1111/j.1432-1033.1991.tb16138.x. [DOI] [PubMed] [Google Scholar]
  7. Kaletta C., Entian K. D., Kellner R., Jung G., Reis M., Sahl H. G. Pep5, a new lantibiotic: structural gene isolation and prepeptide sequence. Arch Microbiol. 1989;152(1):16–19. doi: 10.1007/BF00447005. [DOI] [PubMed] [Google Scholar]
  8. Kaletta C., Entian K. D. Nisin, a peptide antibiotic: cloning and sequencing of the nisA gene and posttranslational processing of its peptide product. J Bacteriol. 1989 Mar;171(3):1597–1601. doi: 10.1128/jb.171.3.1597-1601.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Piard J. C., Kuipers O. P., Rollema H. S., Desmazeaud M. J., de Vos W. M. Structure, organization, and expression of the lct gene for lacticin 481, a novel lantibiotic produced by Lactococcus lactis. J Biol Chem. 1993 Aug 5;268(22):16361–16368. [PubMed] [Google Scholar]
  10. Rauch P. J., De Vos W. M. Characterization of the novel nisin-sucrose conjugative transposon Tn5276 and its insertion in Lactococcus lactis. J Bacteriol. 1992 Feb;174(4):1280–1287. doi: 10.1128/jb.174.4.1280-1287.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ross K. F., Ronson C. W., Tagg J. R. Isolation and characterization of the lantibiotic salivaricin A and its structural gene salA from Streptococcus salivarius 20P3. Appl Environ Microbiol. 1993 Jul;59(7):2014–2021. doi: 10.1128/aem.59.7.2014-2021.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schnell N., Entian K. D., Götz F., Hörner T., Kellner R., Jung G. Structural gene isolation and prepeptide sequence of gallidermin, a new lanthionine containing antibiotic. FEMS Microbiol Lett. 1989 Apr;49(2-3):263–267. doi: 10.1016/0378-1097(89)90050-5. [DOI] [PubMed] [Google Scholar]
  13. Schnell N., Entian K. D., Schneider U., Götz F., Zähner H., Kellner R., Jung G. Prepeptide sequence of epidermin, a ribosomally synthesized antibiotic with four sulphide-rings. Nature. 1988 May 19;333(6170):276–278. doi: 10.1038/333276a0. [DOI] [PubMed] [Google Scholar]
  14. Tagg J. R., Skjold S. A. A bacteriocin produced by certain M-type 49 Streptococcus pyogenes strains when incubated anaerobically. J Hyg (Lond) 1984 Oct;93(2):339–344. doi: 10.1017/s0022172400064883. [DOI] [PMC free article] [PubMed] [Google Scholar]

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