Abstract
To determine which methanogens occur in digestors, we performed a quantitative immunologic analysis of a variety of samples. A comprehensive panel of calibrated polyclonal antibody probes of predefined specificity spectra was used. This allowed precise identification of bacteria by antigenic fingerprinting. A considerable diversity of methanogens was uncovered, much larger than previously reported, encompassing at least 14 strains of 11 species. Strategies were developed to measure the load of any given methanogen in a sample and to compare samples quantitatively. Two methanogens were found to predominate which were antigenically closely related with either Methanobacterium formicicum MF or Methanobrevibacter arboriphilus AZ. Fundamental data, probes, and methods are now available to monitor methanogenic subpopulations during digestor operation and thus learn about their respective roles and predictive significance.
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- Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. Methanogens: reevaluation of a unique biological group. Microbiol Rev. 1979 Jun;43(2):260–296. doi: 10.1128/mr.43.2.260-296.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chartrain M., Zeikus J. G. Microbial ecophysiology of whey biomethanation: characterization of bacterial trophic populations and prevalent species in continuous culture. Appl Environ Microbiol. 1986 Jan;51(1):188–196. doi: 10.1128/aem.51.1.188-196.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Conway de Macario E., Macario A. J., Jovell R. J. Slide immunoenzymatic assay (SIA) in hybridoma technology. Methods Enzymol. 1986;121:509–525. doi: 10.1016/0076-6879(86)21051-4. [DOI] [PubMed] [Google Scholar]
- Conway de Macario E., Macario A. J., Kandler O. Monoclonal antibodies for immunochemical analysis of methanogenic bacteria. J Immunol. 1982 Oct;129(4):1670–1674. [PubMed] [Google Scholar]
- Henson J. M., Smith P. H., White D. C. Examination of thermophilic methane-producing digesters by analysis of bacterial lipids. Appl Environ Microbiol. 1985 Dec;50(6):1428–1433. doi: 10.1128/aem.50.6.1428-1433.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robinson R. W., Akin D. E., Nordstedt R. A., Thomas M. V., Aldrich H. C. Light and electron microscopic examinations of methane-producing biofilms from anaerobic fixed-bed reactors. Appl Environ Microbiol. 1984 Jul;48(1):127–136. doi: 10.1128/aem.48.1.127-136.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Koning H. W., Smith K. R., Last J. M. Biomass fuel combustion and health. Bull World Health Organ. 1985;63(1):11–26. [PMC free article] [PubMed] [Google Scholar]