Skip to main content
PMC home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1976 Apr;33(4):392–399. doi: 10.1038/bjc.1976.64

Stimulation of cellular ingestion by basic proteins in vitro.

F R Westwood, E Longstaff
PMCID: PMC2025076  PMID: 1268031

Abstract

The ingestion of carbon and benzpyrene particles in vitro by rat peritoneal macrophages, baby hamster kidney fibroblasts (BHK-21) and mouse L-cells has been shown to be significantly stimulated by the inclusion of histone or polylysine in the culture medium. Parallel studies using methylated bovine albumin did not significantly stimulate carbon or benzpyrene uptake relative to untreated control cultures. Incubation of carbon particles with histone before inclusion in the culture medium of macrophages resulted in the same degree of uptake as in the cultures where carbon and histone were added independently of each other. The implications of these findings to in vivo chemical carcinogenesis are examined.

Full text

PDF
393

Images in this article

395Fig. 4(a)
on p.395
395Fig. 4(b)
on p.395
396Fig. 4(c)
on p.396
397Fig. 5
on p.397

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLISON A. C., MALLUCCI L. UPTAKE OF HYDROCARBON CARCINOGENS BY LYSOSOMES. Nature. 1964 Sep 5;203:1024–1027. doi: 10.1038/2031024b0. [DOI] [PubMed] [Google Scholar]
  2. Bases R., Mendez F., Mendez L., Angistein R. Stimulation of HeLa cell-surface attachment by histones. Exp Cell Res. 1973 Feb;76(2):441–444. doi: 10.1016/0014-4827(73)90397-2. [DOI] [PubMed] [Google Scholar]
  3. Brunette D. M., Katz M. The interactions of benzo(a)pyrene with cell membranes: uptake into Chinese hamster ovary (CHO) cells and fluorescence studies with isolated membranes. Chem Biol Interact. 1975 Jul;11(1):1–14. doi: 10.1016/0009-2797(75)90061-7. [DOI] [PubMed] [Google Scholar]
  4. Cohn Z. A., Parks E. The regulation of pinocytosis in mouse macrophages. II. Factors inducing vesicle formation. J Exp Med. 1967 Feb 1;125(2):213–232. doi: 10.1084/jem.125.2.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Conning D. M., Firth J. Toxicity of polypropylene in tissue culture. Food Cosmet Toxicol. 1969 Sep;7(5):461–472. doi: 10.1016/s0015-6264(69)80426-8. [DOI] [PubMed] [Google Scholar]
  6. DE VRIES A., SALGO J., MATOTH Y., NEVO A., KATCHALSKI E. Effect of basic polyamino acids on phagocytosis in vitro. Arch Int Pharmacodyn Ther. 1955 Nov 1;104(1):1–10. [PubMed] [Google Scholar]
  7. Lagunoff D., Benditt E. P. Macrophage pinocytosis: the removal and resynthesis of a cell surface factor. Proc Soc Exp Biol Med. 1971 Oct;138(1):118–123. doi: 10.3181/00379727-138-35843. [DOI] [PubMed] [Google Scholar]
  8. Latner A. L., Longstaff E. Transformation of mammalian cells by crude histones. Br J Cancer. 1971 Jun;25(2):280–283. doi: 10.1038/bjc.1971.35. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lavelle S. M. Action of histone on neoplastic and normal growth. Ir J Med Sci. 1973 Mar;142(2):58–65. doi: 10.1007/BF02949991. [DOI] [PubMed] [Google Scholar]
  10. Mazia D., Schatten G., Sale W. Adhesion of cells to surfaces coated with polylysine. Applications to electron microscopy. J Cell Biol. 1975 Jul;66(1):198–200. doi: 10.1083/jcb.66.1.198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ryser H. J., Hancock R. Histones and basic polyamino acids stimulate the uptake of albumin by tumor cells in culture. Science. 1965 Oct 22;150(3695):501–503. doi: 10.1126/science.150.3695.501. [DOI] [PubMed] [Google Scholar]
  12. Styles J. A., Wilson J. Comparison between in vitro toxicity of polymer and mineral dusts and their fibrogenicity. Ann Occup Hyg. 1973 Nov;16(3):241–250. doi: 10.1093/annhyg/16.3.241. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES