Abstract
Human and rat normal tissues and tumours have been studied for the presence of toxic substances, possibly of importance in the development of cachexia in patients with cancer and other chronic diseases. The toxic effect of tissue extracts was gauged by measuring the inhibition of growth of mouse L-cells in 1-ml cultures, as revealed by reduced incorporation of [14C]leucine into cell protein. A common cytotoxic substance of mol. wt. approximately 700 daltons was isolated from all rat and human tissues tested, including tumours. The isolation procedure involved tissue homogenization, followed by pressure dialysis, gel filtration of concentrated pressure dialysates, cation exchange chromatography, and thin layer chromatography. Amounts isolated from different tissues varied by a factor of 3. The purified substance reacted with ninhydrin and a few other reagents for amino groups. It was completely resistant to acid and enzymatic hydrolysis. The evidence thus suggests that the substance is an amine. It is toxic to L-cells, HeLa cells and normal rat fibroblasts in concentrations of 10-20 muM, producing cell death and lysis during incubation overnight.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bloom B. R. In vitro approaches to the mechanism of cell-mediated immune reactions. Adv Immunol. 1971;13:101–208. doi: 10.1016/s0065-2776(08)60184-4. [DOI] [PubMed] [Google Scholar]
- Holmberg B. Further biochemical studies on a dialysable polypeptide obtained from tumor fluids. Eur J Cancer. 1968 Jul;4(3):263–269. doi: 10.1016/0014-2964(68)90051-0. [DOI] [PubMed] [Google Scholar]
- Lozzio B. B., Lozzio C. B., Bamberger E. G., Lair S. V. Regulators of cell division: endogenous mitotic inhibitors of mammalian cells. Int Rev Cytol. 1975;42:1–47. doi: 10.1016/s0074-7696(08)60977-8. [DOI] [PubMed] [Google Scholar]
- Shapot V. S. Some biochemical aspects of the relationship between the tumor and the host. Adv Cancer Res. 1972;15:253–286. doi: 10.1016/s0065-230x(08)60377-2. [DOI] [PubMed] [Google Scholar]
- Steel G. G. Cell loss as a factor in the growth rate of human tumours. Eur J Cancer. 1967 Nov;3(4):381–387. doi: 10.1016/0014-2964(67)90022-9. [DOI] [PubMed] [Google Scholar]
- WISEMAN G., GHADIALLY F. N. A biochemical concept of tumour growth, infiltration, and cachexia. Br Med J. 1958 Jul 5;2(5087):18–21. doi: 10.1136/bmj.2.5087.18. [DOI] [PMC free article] [PubMed] [Google Scholar]