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. 1988 May;54(5):1182–1187. doi: 10.1128/aem.54.5.1182-1187.1988

Degradation of polycyclic aromatic hydrocarbon compounds under various redox conditions in soil-water systems.

J R Mihelcic 1, R G Luthy 1
PMCID: PMC202624  PMID: 3389811

Abstract

This study evaluated the microbial degradation of naphthol, naphthalene, and acenaphthene, under aerobic, anaerobic, and denitrification conditions in soil-water systems. Chemical degradation of naphthol and naphthalene in the presence of a manganese oxide was also studied. Naphthol, naphthalene, and acenaphthene were degraded microbially under aerobic conditions from initial aqueous-phase concentrations of 9, 7, and 1 mg/liter to nondetectable levels in 3, 10, and 10 days, respectively. Under anaerobic conditions naphthol degraded to nondetectable levels in 15 days, whereas naphthalene and acenaphthene showed no significant degradation over periods of 50 and 70 days, respectively. Under denitrification conditions naphthol, naphthalene, and acenaphthene were degraded from initial aqueous-phase concentrations of 8, 7, and 0.4 mg/liter to nondetectable levels in 16, 45, and 40 days, respectively. Acclimation periods of approximately 2 days under aerobic conditions and 2 weeks under denitrification conditions were observed for both naphthalene and acenaphthene. Abiotic degradation of naphthalen and naphthol were evaluated by reaction with manganese oxide, a minor soil constituent. In the presence of a manganese oxide, naphthalene showed no abiotic degradation over a period of 9 weeks, whereas the aqueous naphthol concentration decreased from 9 mg/liter to nondetectable levels in 9 days. The results of this study show that low-molecular-weight, unsubstituted, polycyclic aromatic hydrocarbons are amenable to microbial degradation in soil-water systems under denitrification conditions.

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Selected References

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  1. Atlas R. M. Microbial degradation of petroleum hydrocarbons: an environmental perspective. Microbiol Rev. 1981 Mar;45(1):180–209. doi: 10.1128/mr.45.1.180-209.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bauer J. E., Capone D. G. Degradation and mineralization of the polycyclic aromatic hydrocarbons anthracene and naphthalene in intertidal marine sediments. Appl Environ Microbiol. 1985 Jul;50(1):81–90. doi: 10.1128/aem.50.1.81-90.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bouwer E. J., McCarty P. L. Transformations of halogenated organic compounds under denitrification conditions. Appl Environ Microbiol. 1983 Apr;45(4):1295–1299. doi: 10.1128/aem.45.4.1295-1299.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cerniglia C. E., Gibson D. T. Metabolism of naphthalene by cell extracts of Cunninghamella elegans. Arch Biochem Biophys. 1978 Feb;186(1):121–127. doi: 10.1016/0003-9861(78)90471-x. [DOI] [PubMed] [Google Scholar]
  5. Ensley B. D., Gibson D. T., Laborde A. L. Oxidation of naphthalene by a multicomponent enzyme system from Pseudomonas sp. strain NCIB 9816. J Bacteriol. 1982 Mar;149(3):948–954. doi: 10.1128/jb.149.3.948-954.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hambrick G. A., Delaune R. D., Patrick W. H. Effect of Estuarine Sediment pH and Oxidation-Reduction Potential on Microbial Hydrocarbon Degradation. Appl Environ Microbiol. 1980 Aug;40(2):365–369. doi: 10.1128/aem.40.2.365-369.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Heitkamp M. A., Freeman J. P., Cerniglia C. E. Naphthalene biodegradation in environmental microcosms: estimates of degradation rates and characterization of metabolites. Appl Environ Microbiol. 1987 Jan;53(1):129–136. doi: 10.1128/aem.53.1.129-136.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Herbes S. E., Schwall L. R. Microbial transformation of polycyclic aromatic hydrocarbons in pristine and petroleum-contaminated sediments. Appl Environ Microbiol. 1978 Feb;35(2):306–316. doi: 10.1128/aem.35.2.306-316.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jeffrey A. M., Yeh H. J., Jerina D. M., Patel T. R., Davey J. F., Gibson D. T. Initial reactions in the oxidation of naphthalene by Pseudomonas putida. Biochemistry. 1975 Feb 11;14(3):575–584. doi: 10.1021/bi00674a018. [DOI] [PubMed] [Google Scholar]
  10. Mihelcic J. R., Luthy R. G. Microbial degradation of acenaphthene and naphthalene under denitrification conditions in soil-water systems. Appl Environ Microbiol. 1988 May;54(5):1188–1198. doi: 10.1128/aem.54.5.1188-1198.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schocken M. J., Gibson D. T. Bacterial oxidation of the polycyclic aromatic hydrocarbons acenaphthene and acenaphthylene. Appl Environ Microbiol. 1984 Jul;48(1):10–16. doi: 10.1128/aem.48.1.10-16.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Zeyer J., Kuhn E. P., Schwarzenbach R. P. Rapid microbial mineralization of toluene and 1,3-dimethylbenzene in the absence of molecular oxygen. Appl Environ Microbiol. 1986 Oct;52(4):944–947. doi: 10.1128/aem.52.4.944-947.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

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