Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1988 May;54(5):1289–1292. doi: 10.1128/aem.54.5.1289-1292.1988

Structure of an endo-beta-1,4-glucanase gene from Clostridium acetobutylicum P262 showing homology with endoglucanase genes from Bacillus spp.

H Zappe 1, W A Jones 1, D T Jones 1, D R Woods 1
PMCID: PMC202643  PMID: 3389820

Abstract

The nucleotide sequence of an endo-beta-1,4-glucanase gene of Clostridium acetobutylicum contained two putative extended promoter consensus sequences, a Shine-Dalgarno sequence and a TTG initiation codon. The nucleotide sequence of the gene coding for the C-terminal region of this enzyme was not required for activity. Extensive homology in the nucleotide and amino acid sequences of the endoglucanase genes from C. acetobutylicum and Bacillus spp. was demonstrated.

Full text

PDF
1289

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allcock E. R., Woods D. R. Carboxymethyl cellulase and cellobiase production by Clostridium acetobutylicum in an industrial fermentation medium. Appl Environ Microbiol. 1981 Feb;41(2):539–541. doi: 10.1128/aem.41.2.539-541.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Béguin P., Cornet P., Aubert J. P. Sequence of a cellulase gene of the thermophilic bacterium Clostridium thermocellum. J Bacteriol. 1985 Apr;162(1):102–105. doi: 10.1128/jb.162.1.102-105.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fukumori F., Kudo T., Narahashi Y., Horikoshi K. Molecular cloning and nucleotide sequence of the alkaline cellulase gene from the alkalophilic Bacillus sp. strain 1139. J Gen Microbiol. 1986 Aug;132(8):2329–2335. doi: 10.1099/00221287-132-8-2329. [DOI] [PubMed] [Google Scholar]
  4. Fukumori F., Sashihara N., Kudo T., Horikoshi K. Nucleotide sequences of two cellulase genes from alkalophilic Bacillus sp. strain N-4 and their strong homology. J Bacteriol. 1986 Nov;168(2):479–485. doi: 10.1128/jb.168.2.479-485.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Graves M. C., Rabinowitz J. C. In vivo and in vitro transcription of the Clostridium pasteurianum ferredoxin gene. Evidence for "extended" promoter elements in gram-positive organisms. J Biol Chem. 1986 Aug 25;261(24):11409–11415. [PubMed] [Google Scholar]
  6. Grépinet O., Béguin P. Sequence of the cellulase gene of Clostridium thermocellum coding for endoglucanase B. Nucleic Acids Res. 1986 Feb 25;14(4):1791–1799. doi: 10.1093/nar/14.4.1791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Joliff G., Béguin P., Aubert J. P. Nucleotide sequence of the cellulase gene celD encoding endoglucanase D of Clostridium thermocellum. Nucleic Acids Res. 1986 Nov 11;14(21):8605–8613. doi: 10.1093/nar/14.21.8605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jones D. T., Woods D. R. Acetone-butanol fermentation revisited. Microbiol Rev. 1986 Dec;50(4):484–524. doi: 10.1128/mr.50.4.484-524.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lee S. F., Forsberg C. W., Gibbins L. N. Cellulolytic Activity of Clostridium acetobutylicum. Appl Environ Microbiol. 1985 Aug;50(2):220–228. doi: 10.1128/aem.50.2.220-228.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lee S. F., Forsberg C. W., Gibbins L. N. Xylanolytic Activity of Clostridium acetobutylicum. Appl Environ Microbiol. 1985 Oct;50(4):1068–1076. doi: 10.1128/aem.50.4.1068-1076.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lee S. F., Forsberg C. W. Isolation and Some Properties of a beta-d-Xylosidase from Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol. 1987 Apr;53(4):651–654. doi: 10.1128/aem.53.4.651-654.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lee S. F., Forsberg C. W., Rattray J. B. Purification and Characterization of Two Endoxylanases from Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol. 1987 Apr;53(4):644–650. doi: 10.1128/aem.53.4.644-650.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MacKay R. M., Lo A., Willick G., Zuker M., Baird S., Dove M., Moranelli F., Seligy V. Structure of a Bacillus subtilis endo-beta-1,4-glucanase gene. Nucleic Acids Res. 1986 Nov 25;14(22):9159–9170. doi: 10.1093/nar/14.22.9159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McLaughlin J. R., Murray C. L., Rabinowitz J. C. Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus beta-lactamase gene. J Biol Chem. 1981 Nov 10;256(21):11283–11291. [PubMed] [Google Scholar]
  15. Murphy N., McConnell D. J., Cantwell B. A. The DNA sequence of the gene and genetic control sites for the excreted B. subtilis enzyme beta-glucanase. Nucleic Acids Res. 1984 Jul 11;12(13):5355–5367. doi: 10.1093/nar/12.13.5355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Penttilä M., Lehtovaara P., Nevalainen H., Bhikhabhai R., Knowles J. Homology between cellulase genes of Trichoderma reesei: complete nucleotide sequence of the endoglucanase I gene. Gene. 1986;45(3):253–263. doi: 10.1016/0378-1119(86)90023-5. [DOI] [PubMed] [Google Scholar]
  17. Robson L. M., Chambliss G. H. Endo-beta-1,4-glucanase gene of Bacillus subtilis DLG. J Bacteriol. 1987 May;169(5):2017–2025. doi: 10.1128/jb.169.5.2017-2025.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Woese C. R. Bacterial evolution. Microbiol Rev. 1987 Jun;51(2):221–271. doi: 10.1128/mr.51.2.221-271.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wong W. K., Gerhard B., Guo Z. M., Kilburn D. G., Warren A. J., Miller R. C., Jr Characterization and structure of an endoglucanase gene cenA of Cellulomonas fimi. Gene. 1986;44(2-3):315–324. doi: 10.1016/0378-1119(86)90196-4. [DOI] [PubMed] [Google Scholar]
  21. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]
  22. Zappe H., Jones D. T., Woods D. R. Cloning and expression of Clostridium acetobutylicum endoglucanase, cellobiase and amino acid biosynthesis genes in Escherichia coli. J Gen Microbiol. 1986 May;132(5):1367–1372. doi: 10.1099/00221287-132-5-1367. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES