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. 1988 Jun;54(6):1414–1419. doi: 10.1128/aem.54.6.1414-1419.1988

Bacterial metabolism of carbofuran.

G R Chaudhry 1, A N Ali 1
PMCID: PMC202671  PMID: 3415221

Abstract

Fifteen bacteria capable of degrading carbofuran (2,3-dihydro-2,2-dimethyl-7-benzofuranyl methylcarbamate) were isolated from soil samples with a history of pesticide application. All isolates were gram negative and were oxidase- and catalase-positive rods; they occurred singly or as short chains. All of the identified isolates belonged to one of two genera, Pseudomonas and Flavobacterium. They were separated into three groups based on their mode of utilization of carbofuran. Six isolates were placed in group I; these isolates utilized carbofuran as a sole source of nitrogen. Seven isolates were placed in group II; these isolates utilized the pesticide as a sole source of carbon. Isolates of both groups I and II hydrolyzed carbofuran to carbofuran phenol. Two isolates, designated group III, also utilized carbofuran as a sole source of carbon. They degraded the pesticide more rapidly, however, so up to 40% of [14C]carbofuran was lost as 14CO2 in 1 h. The results suggest that these isolates degrade carbofuran by utilizing an oxidative pathway.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chatterjee D. K., Kellogg S. T., Hamada S., Chakrabarty A. M. Plasmid specifying total degradation of 3-chlorobenzoate by a modified ortho pathway. J Bacteriol. 1981 May;146(2):639–646. doi: 10.1128/jb.146.2.639-646.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chaudhry G. R., Ali A. N., Wheeler W. B. Isolation of a methyl parathion-degrading Pseudomonas sp. that possesses DNA homologous to the opd gene from a Flavobacterium sp. Appl Environ Microbiol. 1988 Feb;54(2):288–293. doi: 10.1128/aem.54.2.288-293.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Don R. H., Pemberton J. M. Genetic and physical map of the 2,4-dichlorophenoxyacetic acid-degradative plasmid pJP4. J Bacteriol. 1985 Jan;161(1):466–468. doi: 10.1128/jb.161.1.466-468.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dunn N. W., Gunsalus I. C. Transmissible plasmid coding early enzymes of naphthalene oxidation in Pseudomonas putida. J Bacteriol. 1973 Jun;114(3):974–979. doi: 10.1128/jb.114.3.974-979.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fahmy M. A., Fukuto T. R., Myers R. O., March R. B. The selective toxicity of new N-phosphorothioyl-carbamate esters. J Agric Food Chem. 1970 Sep-Oct;18(5):793–796. doi: 10.1021/jf60171a014. [DOI] [PubMed] [Google Scholar]
  6. Felsot A., Maddox J. V., Bruce W. Enhanced microbial degradation of carbofuran in soils with histories of Furadan use. Bull Environ Contam Toxicol. 1981 Jun;26(6):781–788. doi: 10.1007/BF01622171. [DOI] [PubMed] [Google Scholar]
  7. Inouye S., Nakazawa A., Nakazawa T. Nucleotide sequence of the regulatory gene xylS on the Pseudomonas putida TOL plasmid and identification of the protein product. Gene. 1986;44(2-3):235–242. doi: 10.1016/0378-1119(86)90187-3. [DOI] [PubMed] [Google Scholar]
  8. Inouye S., Nakazawa A., Nakazawa T. Overproduction of the xylS gene product and activation of the xylDLEGF operon on the TOL plasmid. J Bacteriol. 1987 Aug;169(8):3587–3592. doi: 10.1128/jb.169.8.3587-3592.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Pemberton J. M., Fisher P. R. 2,4-D plasmids and persistence. Nature. 1977 Aug 25;268(5622):732–733. doi: 10.1038/268732a0. [DOI] [PubMed] [Google Scholar]
  11. Spooner R. A., Lindsay K., Franklin F. C. Genetic, functional and sequence analysis of the xylR and xylS regulatory genes of the TOL plasmid pWW0. J Gen Microbiol. 1986 May;132(5):1347–1358. doi: 10.1099/00221287-132-5-1347. [DOI] [PubMed] [Google Scholar]
  12. Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]
  13. Streber W. R., Timmis K. N., Zenk M. H. Analysis, cloning, and high-level expression of 2,4-dichlorophenoxyacetate monooxygenase gene tfdA of Alcaligenes eutrophus JMP134. J Bacteriol. 1987 Jul;169(7):2950–2955. doi: 10.1128/jb.169.7.2950-2955.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Venkateswarlu K., Gowda T. K., Sethunathan N. Persistence and biodegradation of carbofuran in flooded soils. J Agric Food Chem. 1977 May-Jun;25(3):533–536. doi: 10.1021/jf60211a017. [DOI] [PubMed] [Google Scholar]
  15. Williams I. H., Pepin H. S., Brown M. J. Degradation of carbofuran by soil microorganisms. Bull Environ Contam Toxicol. 1976 Feb;15(2):244–249. doi: 10.1007/BF01685169. [DOI] [PubMed] [Google Scholar]
  16. Williams P. A., Murray K. Metabolism of benzoate and the methylbenzoates by Pseudomonas putida (arvilla) mt-2: evidence for the existence of a TOL plasmid. J Bacteriol. 1974 Oct;120(1):416–423. doi: 10.1128/jb.120.1.416-423.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wong C. L., Dunn N. W. Transmissible plasmid coding for the degradation of benzoate and m-toluate in Pseudomonas arvilla mt-2. Genet Res. 1974 Apr;23(2):227–232. doi: 10.1017/s0016672300014853. [DOI] [PubMed] [Google Scholar]
  18. Worsey M. J., Franklin F. C., Williams P. A. Regulation of the degradative pathway enzymes coded for by the TOL plasmid (pWWO) from Pseudomonas putida mt-2. J Bacteriol. 1978 Jun;134(3):757–764. doi: 10.1128/jb.134.3.757-764.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Worsey M. J., Williams P. A. Metabolism of toluene and xylenes by Pseudomonas (putida (arvilla) mt-2: evidence for a new function of the TOL plasmid. J Bacteriol. 1975 Oct;124(1):7–13. doi: 10.1128/jb.124.1.7-13.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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