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. 1988 Sep;54(9):2325–2327. doi: 10.1128/aem.54.9.2325-2327.1988

Determination of Poly-β-Hydroxybutyrate and Poly-β-Hydroxyvalerate in Activated Sludge by Gas-Liquid Chromatography

Yves Comeau 1,*, Kenneth J Hall 1, William K Oldham 1
PMCID: PMC202859  PMID: 16347745

Abstract

A convenient gas-liquid chromatography procedure to quantify poly-β-hydroxybutyrate and poly-β-hydroxyvalerate in activated sludge was developed by combining lyophilization of the samples, purification of the chloroform phase by water reextraction, and the use of capillary columns. With a flame ionization detector the sensitivity was estimated at 10−5 g/liter.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dawes E. A., Senior P. J. The role and regulation of energy reserve polymers in micro-organisms. Adv Microb Physiol. 1973;10:135–266. doi: 10.1016/s0065-2911(08)60088-0. [DOI] [PubMed] [Google Scholar]
  2. Findlay R. H., White D. C. Polymeric Beta-Hydroxyalkanoates from Environmental Samples and Bacillus megaterium. Appl Environ Microbiol. 1983 Jan;45(1):71–78. doi: 10.1128/aem.45.1.71-78.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. LAW J. H., SLEPECKY R. A. Assay of poly-beta-hydroxybutyric acid. J Bacteriol. 1961 Jul;82:33–36. doi: 10.1128/jb.82.1.33-36.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Nickerson K. W. Purification of Poly-beta-Hydroxybutyrate by Density Gradient Centrifugation in Sodium Bromide. Appl Environ Microbiol. 1982 May;43(5):1208–1209. doi: 10.1128/aem.43.5.1208-1209.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Odham G., Tunlid A., Westerdahl G., Mårdén P. Combined Determination of Poly-beta-Hydroxyalkanoic and Cellular Fatty Acids in Starved Marine Bacteria and Sewage Sludge by Gas Chromatography with Flame Ionization or Mass Spectrometry Detection. Appl Environ Microbiol. 1986 Oct;52(4):905–910. doi: 10.1128/aem.52.4.905-910.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. WILLIAMSON D. H., WILKINSON J. F. The isolation and estimation of the poly-beta-hydroxybutyrate inclusions of Bacillus species. J Gen Microbiol. 1958 Aug;19(1):198–209. doi: 10.1099/00221287-19-1-198. [DOI] [PubMed] [Google Scholar]
  7. Ward A. C., Dawes E. A. A disk assay for poly- -hydroxybutyrate. Anal Biochem. 1973 Apr;52(2):607–613. doi: 10.1016/0003-2697(73)90067-5. [DOI] [PubMed] [Google Scholar]

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