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. 1989 Jun;55(6):1649–1652. doi: 10.1128/aem.55.6.1649-1652.1989

Transformation of Bacillus cereus vegetative cells by electroporation.

B H Belliveau 1, J T Trevors 1
PMCID: PMC202922  PMID: 2504113

Abstract

Transformation of untreated vegetative cells of Bacillus cereus 569 with plasmid pC194 (1.8 megadaltons) by high-voltage electroporation resulted in a maximum of 2 x 10(-5) transformants per viable cell. Transformation of a 130-megadalton plasmid occurred at a comparable frequency. The method was simple, rapid, and yielded transformant colonies in 14 to 24 h. Transformation was obtained with unpurified total plasmid DNA.

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Selected References

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