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. 1989 Aug;55(8):1963–1967. doi: 10.1128/aem.55.8.1963-1967.1989

Kinetic Analysis of Protein Degradation by a Freshwater Wetland Sediment Community

H W Cunningham 1, Robert G Wetzel 1,*
PMCID: PMC202988  PMID: 16347988

Abstract

The mineralization of proteins by the sediment microflora of a freshwater wetland conformed to a kinetic model developed for polymer degradation. The maximum velocity of protein mineralization ranged from 2,078 to 147 nmol of protein cm−3 h−1 from May to October. The turnover time of protein was 13 to 69 h. A statistical comparison of the kinetic parameters by the standard error of the estimate demonstrated that protein degradation exhibited significant random fluctuations throughout the growing season. These fluctuations were related to changes in the concentration of readily degradable protein as estimated by Kt. Utilization of amino acids was 9 to 57 times greater than the utilization of protein, and the turnover time for amino acids was 1.6 to 3.7 h.

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1966

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cornish-Bowden A., Eisenthal R. Statistical considerations in the estimation of enzyme kinetic parameters by the direct linear plot andother methods. Biochem J. 1974 Jun;139(3):721–730. doi: 10.1042/bj1390721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Eisenthal R., Cornish-Bowden A. The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters. Biochem J. 1974 Jun;139(3):715–720. doi: 10.1042/bj1390715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Harrison M. J., Wright R. T., Morita R. Y. Method for measuring mineralization in lake sediments. Appl Microbiol. 1971 Apr;21(4):698–702. doi: 10.1128/am.21.4.698-702.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. McClure W. R. A kinetic analysis of coupled enzyme assays. Biochemistry. 1969 Jul;8(7):2782–2786. doi: 10.1021/bi00835a014. [DOI] [PubMed] [Google Scholar]
  5. Siegel A., Degens E. T. Concentration of dissolved amino acids from saline waters by ligand-exchange chromatography. Science. 1966 Mar 4;151(3714):1098–1101. doi: 10.1126/science.151.3714.1098. [DOI] [PubMed] [Google Scholar]
  6. WILKINSON G. N. Statistical estimations in enzyme kinetics. Biochem J. 1961 Aug;80:324–332. doi: 10.1042/bj0800324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Wright R. T. Measurement and significance of specific activity in the heterotrophic bacteria of natural waters. Appl Environ Microbiol. 1978 Aug;36(2):297–305. doi: 10.1128/aem.36.2.297-305.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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