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. 1989 Aug;55(8):2105–2107. doi: 10.1128/aem.55.8.2105-2107.1989

The K1 Toxin of Saccharomyces cerevisiae Kills Spheroplasts of Many Yeast Species

Hong Zhu 1, Howard Bussey 1,*
PMCID: PMC203014  PMID: 16348001

Abstract

The Saccharomyces cerevisiae K1 toxin killed spheroplasts from the genera Candida, Kluyveromyces, and Schwanniomyces. Cells of these organisms were toxin insensitive. The toxin bound poorly to Kluyveromyces lactis cells. In contrast, Candida albicans bound the toxin to an extent similar to that seen with S. cerevisiae. Thus, wall receptors can define toxin specificity and are necessary but not sufficient for toxin action on intact cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Al-Aidroos K., Bussey H. Chromosomal mutants of Saccharomyces cerevisiae affecting the cell wall binding site for killer factor. Can J Microbiol. 1978 Mar;24(3):228–237. doi: 10.1139/m78-041. [DOI] [PubMed] [Google Scholar]
  2. Bostian K. A., Elliott Q., Bussey H., Burn V., Smith A., Tipper D. J. Sequence of the preprotoxin dsRNA gene of type I killer yeast: multiple processing events produce a two-component toxin. Cell. 1984 Mar;36(3):741–751. doi: 10.1016/0092-8674(84)90354-4. [DOI] [PubMed] [Google Scholar]
  3. Bussey H. Physiology of killer factor in yeast. Adv Microb Physiol. 1981;22:93–122. doi: 10.1016/s0065-2911(08)60326-4. [DOI] [PubMed] [Google Scholar]
  4. Bussey H., Skipper N. Killing of Torulopsis glabrata by Saccharomyces cerevisiae killer factor. Antimicrob Agents Chemother. 1976 Feb;9(2):352–354. doi: 10.1128/aac.9.2.352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gopal P. K., Shepherd M. G., Sullivan P. A. Analysis of wall glucans from yeast, hyphal and germ-tube forming cells of Candida albicans. J Gen Microbiol. 1984 Dec;130(12):3295–3301. doi: 10.1099/00221287-130-12-3295. [DOI] [PubMed] [Google Scholar]
  6. Hinnen A., Hicks J. B., Fink G. R. Transformation of yeast. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929–1933. doi: 10.1073/pnas.75.4.1929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hutchins K., Bussey H. Cell wall receptor for yeast killer toxin: involvement of (1 leads to 6)-beta-D-glucan. J Bacteriol. 1983 Apr;154(1):161–169. doi: 10.1128/jb.154.1.161-169.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kagan B. L. Mode of action of yeast killer toxins: channel formation in lipid bilayer membranes. Nature. 1983 Apr 21;302(5910):709–711. doi: 10.1038/302709a0. [DOI] [PubMed] [Google Scholar]
  9. Tipper D. J., Bostian K. A. Double-stranded ribonucleic acid killer systems in yeasts. Microbiol Rev. 1984 Jun;48(2):125–156. doi: 10.1128/mr.48.2.125-156.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Young T. W., Yagiu M. A comparison of the killer character in different yeasts and its classification. Antonie Van Leeuwenhoek. 1978;44(1):59–77. doi: 10.1007/BF00400077. [DOI] [PubMed] [Google Scholar]
  11. Zhu H., Bussey H., Thomas D. Y., Gagnon J., Bell A. W. Determination of the carboxyl termini of the alpha and beta subunits of yeast K1 killer toxin. Requirement of a carboxypeptidase B-like activity for maturation. J Biol Chem. 1987 Aug 5;262(22):10728–10732. [PubMed] [Google Scholar]
  12. de la Peña P., Barros F., Gascón S., Lazo P. S., Ramos S. Effect of yeast killer toxin on sensitive cells of Saccharomyces cerevisiae. J Biol Chem. 1981 Oct 25;256(20):10420–10425. [PubMed] [Google Scholar]

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