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. 1975 Dec;81(3):575–588.

Isolation and partial characterization of human eosinophil granules. Comparison to neutrophils.

B C West, N A Gelb, A S Rosenthal
PMCID: PMC2032331  PMID: 1211424

Abstract

Human blood eosinophils obtained from untreated patients with large numbers of circulating eosinophils were purified and lysed. An eosinophil contains 2.65 times as much peroxidase, 2.44 times as much beta-glucuronidase, approximately two times as much acid beta-glycerophosphatase, and 1.2 times as much protein as a neutrophil. Lysate filtration allowed isolation of eosinophil granules by isopycnic ultracentrifugation in sucrose. The granules had a mean density of rho 1.24 g/ml, and contained peroxidase, beta-glucuronidase, and acid beta-glycerophosphatase. They totally lacked muramidase and alkaline phosphatase. Electron micrography confirmed the isolation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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