Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1972 Feb;66(2):225–240.

Myocardial Fibrosis and Smooth Muscle Cell Hyperplasia in Coronary Arteries of Allylamine-Fed Rats

J J Lalich, J R Allen, W C W Paik
PMCID: PMC2032491  PMID: 5009971

Abstract

Immature female Long-Evans rats were fed 2 g of allylamine-HCl kg of commercial diet for periods of 84—281 days. Coronary arteries and myocardium were examined in 16 control and 23 test rats. Cellular alterations in the arterial tributaries were found principally proximal to or within the areas of myocardial fibrosis. Whereas intimal smooth muscle cell (SMC) hyperplasia was prominent in vessels of smaller caliber, medial hyalinosis was seen frequently in arteries with diameters greater than 200 μ. Intimal hyperplasia developed in the peripheral coronary branches without any evidence of leukocytic infiltration or thrombus formation. It appeared that SMC hyperplasia in the intima contributed more often to a reduction of luminal patency than medial hyalinosis in allylamine-fed rats. On the basis of alterations in the coronary arteries and the localization of fibrosis, we believe that hypoxia is the cause of myocardial necrosis.

Full text

PDF

Images in this article

239Fig 9
on p.239
239Fig 10
on p.239
240Fig 11
on p.240
240Fig 12
on p.240
235Fig 1
on p.235
235Fig 2
on p.235
236Fig 3
on p.236
236Fig 4
on p.236
237Fig 5
on p.237
237Fig 6
on p.237
238Fig 7
on p.238
238Fig 8
on p.238

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLOOR C. M., LOWMAN R. M. EXPERIMENTAL CORONARY ARTERIOGRAPHY. I. THE DISTRIBUTION AND EXTENT OF ALLYLAMINE-INDUCED VASCULAR LESIONS IN THE DOG. Radiology. 1963 Nov;81:770–777. doi: 10.1148/81.5.770. [DOI] [PubMed] [Google Scholar]
  2. CAULFIELD J. B. Effects of varying the vehicle for OsO4 in tissue fixation. J Biophys Biochem Cytol. 1957 Sep 25;3(5):827–830. doi: 10.1083/jcb.3.5.827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CONRAD L. L., GONZALEZ I. E., JOEL W., FURMAN R. H. Histochemical evaluation of canine coronary artery and aortic lesions induced by intravenous allylamine. Circ Res. 1956 May;4(3):263–267. doi: 10.1161/01.res.4.3.263. [DOI] [PubMed] [Google Scholar]
  4. Carstairs K. C. The identification of platelets and platelet antigens in histological sections. J Pathol Bacteriol. 1965 Jul;90(1):225–231. doi: 10.1002/path.1700900124. [DOI] [PubMed] [Google Scholar]
  5. Constantinides P., Robinson M. Ultrastructural injury of arterial endothelium. II. Effects of vasoactive amines. Arch Pathol. 1969 Aug;88(2):106–112. [PubMed] [Google Scholar]
  6. Dbalý J., Ostádal B., Rychter Z. Development of the coronary arteries in rat embryos. Acta Anat (Basel) 1968;71(2):209–222. doi: 10.1159/000143186. [DOI] [PubMed] [Google Scholar]
  7. GOMORI G. A rapid one-step trichrome stain. Am J Clin Pathol. 1950 Jul;20(7):661–664. doi: 10.1093/ajcp/20.7_ts.661. [DOI] [PubMed] [Google Scholar]
  8. GUZMAN R. J., LOQUVAM G. S., KODAMA J. K., HINE C. H. Myocarditis produced by allylamines. Experimental. Arch Environ Health. 1961 Jan;2:62–73. doi: 10.1080/00039896.1961.10662817. [DOI] [PubMed] [Google Scholar]
  9. HALPERN M. H. The dual blood supply of the rat heart. Am J Anat. 1957 Jul;101(1):1–16. doi: 10.1002/aja.1001010102. [DOI] [PubMed] [Google Scholar]
  10. HARTROFT W. S., THOMAS W. A. Pathological lesions related to disturbances of fat and cholesterol metabolism in man. J Am Med Assoc. 1957 Aug 24;164(17):1899–1905. doi: 10.1001/jama.1957.62980170011007c. [DOI] [PubMed] [Google Scholar]
  11. HORST A., ROZYNKOWA D., ZAGORSKA I. Changes in the aorta of the rat after the injection of allylamines and egg-yolk. Acta Med Pol. 1960;1:1–9. [PubMed] [Google Scholar]
  12. HORT W., DACANALIS S. UNTERSUCHUNGEN AN RATTENHERZEN NACH TEMPORAERER UNTERBINDUNG DER LINKEN KRANZARTERIE. Virchows Arch Pathol Anat Physiol Klin Med. 1965 Mar 31;339:61–71. [PubMed] [Google Scholar]
  13. Heggtveit H. A., Grice H. C., Wiberg G. S. Cobalt cardiomyopathy. Experimental basis for the human lesion. Pathol Microbiol (Basel) 1970;35(1):110–113. [PubMed] [Google Scholar]
  14. Lalich J. J. Coronary artery hyalinosis in rats fed allylamine. Exp Mol Pathol. 1969 Feb;10(1):14–26. doi: 10.1016/0014-4800(69)90045-8. [DOI] [PubMed] [Google Scholar]
  15. MOLLENHAUER H. H. PLASTIC EMBEDDING MIXTURES FOR USE IN ELECTRON MICROSCOPY. Stain Technol. 1964 Mar;39:111–114. [PubMed] [Google Scholar]
  16. Paik W. C., Lalich J. Factors which contribute to aortic fibrous repair in rats fed beta-aminopropionitrile. Lab Invest. 1970 Jan;22(1):28–35. [PubMed] [Google Scholar]
  17. RING P. A. Myocardial regeneration in experimental ischaemic lesions of the heart. J Pathol Bacteriol. 1950 Jan;62(1):21–27. doi: 10.1002/path.1700620103. [DOI] [PubMed] [Google Scholar]
  18. SABATINI D. D., BENSCH K., BARRNETT R. J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol. 1963 Apr;17:19–58. doi: 10.1083/jcb.17.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. TAYLOR C. B., COX G. E., MANALO-ESTRELLA P., SOUTHWORTH J., PATTON D. E., CATHCART C. Atherosclerosis in rhesus monkeys. II. Arterial lesions associated with hypercholesteremia induced by dietary fat and cholesterol. Arch Pathol. 1962 Jul;74:16–34. [PubMed] [Google Scholar]
  20. WATERS L. L. Studies on the pathogenesis of vascular disease: the effect of intravenously injected human plasma and of lipid-rich human plasma globulins on inflammatory lesions of the coronary arteries of dogs. Yale J Biol Med. 1957 Sep;30(1):57–71. [PMC free article] [PubMed] [Google Scholar]
  21. WISSLER R. W., EILERT M. L., SCHROEDER M. A., COHEN L. Production of lipomatous and atheromatous arterial lesions in the albino rat. AMA Arch Pathol. 1954 Apr;57(4):333–351. [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES