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. 1970 Aug;60(2):289–312.

The loss of phenotypic traits by differentiated cells in vitro. VII. Effects of 5-bromodeoxyuridine and prolonged culturing on fine structure of chondrocytes.

H C Anderson, S Chacko, J Abbott, H Holtzer
PMCID: PMC2032892  PMID: 5457215

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abbott J., Holtzer H. The loss of phenotypic traits by differentiated cells, V. The effect of 5-bromodeoxyuridine on cloned chondrocytes. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1144–1151. doi: 10.1073/pnas.59.4.1144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Abbott J., Holtzer H. The loss of phenotypic traits by differentiated cells. 3. The reversible behavior of chondrocytes in primary cultures. J Cell Biol. 1966 Mar;28(3):473–487. doi: 10.1083/jcb.28.3.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Anderson H. C. Electron microscopic studies of induced cartilage development and calcification. J Cell Biol. 1967 Oct;35(1):81–101. doi: 10.1083/jcb.35.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Buckley I. K., Porter K. R. Cytoplasmic fibrils in living cultured cells. A light and electron microscope study. Protoplasma. 1967;64(4):349–380. doi: 10.1007/BF01666538. [DOI] [PubMed] [Google Scholar]
  5. CAMERON D. A., ROBINSON R. A. Electron microscopy of cartilage and bone matrix at the distal epiphyseal line of the femur in the newborn infant. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):253–260. doi: 10.1083/jcb.2.4.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chacko S., Abbott J., Holtzer S., Holtzer H. The loss of phenotypic traits by differentiated cells. VI. Behavior of the progeny of a single chondrocyte. J Exp Med. 1969 Aug 1;130(2):417–442. doi: 10.1084/jem.130.2.417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Coleman J. R., Coleman A. W., Hartline E. J. A clonal study of the reversible inhibition of muscle differentiation by the halogenated thymidine analog 5-bromodeoxyuridine. Dev Biol. 1969 Jun;19(6):527–548. doi: 10.1016/0012-1606(69)90036-0. [DOI] [PubMed] [Google Scholar]
  8. Coon H. G. Clonal stability and phenotypic expression of chick cartilage cells in vitro. Proc Natl Acad Sci U S A. 1966 Jan;55(1):66–73. doi: 10.1073/pnas.55.1.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Engfeldt B. Studies on the epiphysial growth zone. IV. The effect of papain on the ultrastructure of epiphysial cartilage. Acta Pathol Microbiol Scand. 1969;75(2):220–228. [PubMed] [Google Scholar]
  10. GODMAN G. C., LANE N. ON THE SITE OF SULFATION IN THE CHONDROCYTE. J Cell Biol. 1964 Jun;21:353–366. doi: 10.1083/jcb.21.3.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. GODMAN G. C., PORTER K. R. Chondrogenesis, studied with the electron microscope. J Biophys Biochem Cytol. 1960 Dec;8:719–760. doi: 10.1083/jcb.8.3.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. GOLDBERG B., GREEN H. AN ANALYSIS OF COLLAGEN SECRETION BY ESTABLISHED MOUSE FIBROBLAST LINES. J Cell Biol. 1964 Jul;22:227–258. doi: 10.1083/jcb.22.1.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Holthausen H. S., Chacko S., Davidson E. A., Holtzer H. Effect of 5-bromodeoxyuridine on expression of cultured chondrocytes grown in vitro. Proc Natl Acad Sci U S A. 1969 Jul;63(3):864–870. doi: 10.1073/pnas.63.3.864. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Holtzer H., Abbott J., Lash J., Holtzer S. THE LOSS OF PHENOTYPIC TRAITS BY DIFFERENTIATED CELLS IN VITRO, I. DEDIFFERENTIATION OF CARTILAGE CELLS. Proc Natl Acad Sci U S A. 1960 Dec;46(12):1533–1542. doi: 10.1073/pnas.46.12.1533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. JENNINGS M. A., FLOREY H. W. Autoradiographic observations on the mucous cells of the stomach and intestine. Q J Exp Physiol Cogn Med Sci. 1956 Apr;41(2):131–152. doi: 10.1113/expphysiol.1956.sp001171. [DOI] [PubMed] [Google Scholar]
  16. KURODA Y. STUDIES ON CARTILAGE CELLS IN VITRO. I. MORPHOLOGY AND GROWTH OF CARTILAGE CELLS IN MONOLAYER CULTURES. Exp Cell Res. 1964 Jul;35:326–336. doi: 10.1016/0014-4827(64)90099-0. [DOI] [PubMed] [Google Scholar]
  17. Khan T. A., Overton J. Lanthanum staining of developing chick cartilage and reaggregating cartilage cells. J Cell Biol. 1970 Feb;44(2):433–438. doi: 10.1083/jcb.44.2.433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lasher R., Cahn R. D. The effects of 5-Bromodeoxyuridine on the differentiation of chondrocytes in vitro. Dev Biol. 1969 May;19(5):415–435. doi: 10.1016/0012-1606(69)90080-3. [DOI] [PubMed] [Google Scholar]
  19. Matukas V. J., Panner B. J., Orbison J. L. Studies on ultrastructural identification and distribution of protein-polysaccharide in cartilage matrix. J Cell Biol. 1967 Feb;32(2):365–377. doi: 10.1083/jcb.32.2.365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nameroff M., Holtzer H. The loss of phenotypic traits by differentiated cells. IV. Changes in polysaccharides produced by dividing chondrocytes. Dev Biol. 1967 Sep;16(3):250–281. doi: 10.1016/0012-1606(67)90026-7. [DOI] [PubMed] [Google Scholar]
  21. Okazaki K., Holtzer H. An analysis of myogenesis in vitro using fluorescein-labeled antimyosin. J Histochem Cytochem. 1965 Nov-Dec;13(8):726–739. doi: 10.1177/13.8.726. [DOI] [PubMed] [Google Scholar]
  22. PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. PETERSON M., LEBLOND C. P. SYNTHESIS OF COMPLEX CARBOHYDRATES IN THE GOLGI REGION, AS SHOWN BY RADIOAUTOGRAPHY AFTER INJECTION OF LABELED GLUCOSE. J Cell Biol. 1964 Apr;21:143–148. doi: 10.1083/jcb.21.1.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. REVEL J. P., HAY E. D. AN AUTORADIOGRAPHIC AND ELECTRON MICROSCOPIC STUDY OF COLLAGEN SYNTHESIS IN DIFFERENTIATING CARTILAGE. Z Zellforsch Mikrosk Anat. 1963 Oct 8;61:110–144. doi: 10.1007/BF00341524. [DOI] [PubMed] [Google Scholar]
  25. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. SABATINI D. D., BENSCH K., BARRNETT R. J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol. 1963 Apr;17:19–58. doi: 10.1083/jcb.17.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. SIEKEVITZ P., PALADE G. E. A cytochemical study on the pancreas of the guinea pig. 5. In vivo incorporation of leucine-1-C14 into the chymotrypsinogen of various cell fractions. J Biophys Biochem Cytol. 1960 Jul;7:619–630. doi: 10.1083/jcb.7.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. TAKUMA S. Electron microscopy of the developing cartilagenous epiphysis. Arch Oral Biol. 1960 Jul;2:111–119. doi: 10.1016/0003-9969(60)90059-5. [DOI] [PubMed] [Google Scholar]

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