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. 1994 Jul;70(1):97–101. doi: 10.1038/bjc.1994.256

Prediction of accumulation of 131I-labelled meta-iodobenzylguanidine in neuroblastoma cell lines by means of reverse transcription and polymerase chain reaction.

R J Mairs 1, A Livingstone 1, M N Gaze 1, T E Wheldon 1, A Barrett 1
PMCID: PMC2033329  PMID: 7517173

Abstract

Radiolabelled meta-iodobenzylguanidine (mIBG) currently provides one of the most promising options for targeted radiotherapy of neuroblastoma. No means currently exists for prediction of mIBG uptake in tumour cells of individual patients other than semiquantitative inferences from diagnostic scanning which depend on the continued existence of a macroscopic tumour mass. A biological rapid assay which could be applied at initial biopsy would be invaluable in selecting patients for therapeutic strategies which incorporate radiolabelled mIBG. We have assessed the expression of the noradrenaline transporter gene in six human neuroblastoma cell lines and in three non-neural crest-derived cell lines using reverse transcription followed by the polymerase chain reaction. Transcription of this gene was observed in five out of six neuroblastoma cell lines but in none of the control cells. A highly significant correlation was established (P < 0.01) between gene expression and active cellular accumulation of mIBG. It is suggested that semiquantitative evaluation of noradrenaline transporter gene transcripts may be predictive of mIBG uptake by tumours in vivo.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beierwaltes W. H. Treatment of neuroblastoma with 131I-MIBG: dosimetric problems and perspectives. Med Pediatr Oncol. 1987;15(4):188–191. doi: 10.1002/mpo.2950150409. [DOI] [PubMed] [Google Scholar]
  2. Biedler J. L., Helson L., Spengler B. A. Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture. Cancer Res. 1973 Nov;33(11):2643–2652. [PubMed] [Google Scholar]
  3. Biedler J. L., Roffler-Tarlov S., Schachner M., Freedman L. S. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 1978 Nov;38(11 Pt 1):3751–3757. [PubMed] [Google Scholar]
  4. Buck J., Bruchelt G., Girgert R., Treuner J., Niethammer D. Specific uptake of m-[125I]iodobenzylguanidine in the human neuroblastoma cell line SK-N-SH. Cancer Res. 1985 Dec;45(12 Pt 1):6366–6370. [PubMed] [Google Scholar]
  5. Carachi R., Raza T., Robertson D., Wheldon T. W., Wilson L., Livingstone A., van Heyningen V., Spowart G., Middleton P., Gosden J. R. Biological properties of a tumour cell line (NB1-G) derived from human neuroblastoma. Br J Cancer. 1987 Apr;55(4):407–411. doi: 10.1038/bjc.1987.80. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Corbett R., Pinkerton R., Pritchard J., Meller S., Lewis I., Kingston J., McElwain T. Pilot study of high-dose vincristine, etoposide, carboplatin and melphalan with autologous bone marrow rescue in advanced neuroblastoma. Eur J Cancer. 1992;28A(8-9):1324–1328. doi: 10.1016/0959-8049(92)90509-z. [DOI] [PubMed] [Google Scholar]
  7. Feine U., Müller-Schauenburg W., Treuner J., Klingebiel T. Metaiodobenzylguanidine (MIBG) labeled with 123I/131I in neuroblastoma diagnosis and follow-up treatment with a review of the diagnostic results of the International Workshop of Pediatric Oncology held in Rome, September 1986. Med Pediatr Oncol. 1987;15(4):181–187. doi: 10.1002/mpo.2950150408. [DOI] [PubMed] [Google Scholar]
  8. Fielding S. L., Flower M. A., Ackery D. M., Kemshead J., Lashford L. S., Lewis I. J. The treatment of resistant neuroblastoma with 131I-mIBG: alternative methods of dose prescription. Radiother Oncol. 1992 Sep;25(1):73–76. doi: 10.1016/0167-8140(92)90199-5. [DOI] [PubMed] [Google Scholar]
  9. Gonzalez I. L., Gorski J. L., Campen T. J., Dorney D. J., Erickson J. M., Sylvester J. E., Schmickel R. D. Variation among human 28S ribosomal RNA genes. Proc Natl Acad Sci U S A. 1985 Nov;82(22):7666–7670. doi: 10.1073/pnas.82.22.7666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hamilton T. C., Young R. C., McKoy W. M., Grotzinger K. R., Green J. A., Chu E. W., Whang-Peng J., Rogan A. M., Green W. R., Ozols R. F. Characterization of a human ovarian carcinoma cell line (NIH:OVCAR-3) with androgen and estrogen receptors. Cancer Res. 1983 Nov;43(11):5379–5389. [PubMed] [Google Scholar]
  11. Huddart S. N., Mann J. R., McGukin A. G., Corbett R. MYCN amplification by differential PCR. Pediatr Hematol Oncol. 1993 Jan-Mar;10(1):31–34. doi: 10.3109/08880019309016525. [DOI] [PubMed] [Google Scholar]
  12. Jaques S., Jr, Tobes M. C., Sisson J. C. Sodium dependency of uptake of norepinephrine and m-iodobenzylguanidine into cultured human pheochromocytoma cells: evidence for uptake-one. Cancer Res. 1987 Aug 1;47(15):3920–3928. [PubMed] [Google Scholar]
  13. Lashford L. S., Hancock J. P., Kemshead J. T. Meta-iodobenzylguanidine (mIBG) uptake and storage in the human neuroblastoma cell line SK-N-BE(2C). Int J Cancer. 1991 Jan 2;47(1):105–109. doi: 10.1002/ijc.2910470119. [DOI] [PubMed] [Google Scholar]
  14. Lashford L. S., Lewis I. J., Fielding S. L., Flower M. A., Meller S., Kemshead J. T., Ackery D. Phase I/II study of iodine 131 metaiodobenzylguanidine in chemoresistant neuroblastoma: a United Kingdom Children's Cancer Study Group investigation. J Clin Oncol. 1992 Dec;10(12):1889–1896. doi: 10.1200/JCO.1992.10.12.1889. [DOI] [PubMed] [Google Scholar]
  15. Mairs R. J., Gaze M. N., Barrett A. The uptake and retention of metaiodobenzyl guanidine by the neuroblastoma cell line NB1-G. Br J Cancer. 1991 Aug;64(2):293–295. doi: 10.1038/bjc.1991.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mattano L. A., Jr, Moss T. J., Emerson S. G. Sensitive detection of rare circulating neuroblastoma cells by the reverse transcriptase-polymerase chain reaction. Cancer Res. 1992 Sep 1;52(17):4701–4705. [PubMed] [Google Scholar]
  17. Montaldo P. G., Carbone R., Ponzoni M., Cornaglia-Ferraris P. gamma-Interferon increases metaiodobenzylguanidine incorporation and retention in human neuroblastoma cells. Cancer Res. 1992 Sep 15;52(18):4960–4964. [PubMed] [Google Scholar]
  18. Montaldo P. G., Lanciotti M., Casalaro A., Cornaglia-Ferraris P., Ponzoni M. Accumulation of m-iodobenzylguanidine by neuroblastoma cells results from independent uptake and storage mechanisms. Cancer Res. 1991 Aug 15;51(16):4342–4346. [PubMed] [Google Scholar]
  19. Moyes J. S., Babich J. W., Carter R., Meller S. T., Agrawal M., McElwain T. J. Quantitative study of radioiodinated metaiodobenzylguanidine uptake in children with neuroblastoma: correlation with tumor histopathology. J Nucl Med. 1989 Apr;30(4):474–480. [PubMed] [Google Scholar]
  20. Pacholczyk T., Blakely R. D., Amara S. G. Expression cloning of a cocaine- and antidepressant-sensitive human noradrenaline transporter. Nature. 1991 Mar 28;350(6316):350–354. doi: 10.1038/350350a0. [DOI] [PubMed] [Google Scholar]
  21. SCHERER W. F., SYVERTON J. T., GEY G. O. Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix. J Exp Med. 1953 May;97(5):695–710. doi: 10.1084/jem.97.5.695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schlesinger H. R., Gerson J. M., Moorhead P. S., Maguire H., Hummeler K. Establishment and characterization of human neuroblastoma cell lines. Cancer Res. 1976 Sep;36(9 PT1):3094–3100. [PubMed] [Google Scholar]
  23. Shapiro B., Copp J. E., Sisson J. C., Eyre P. L., Wallis J., Beierwaltes W. H. Iodine-131 metaiodobenzylguanidine for the locating of suspected pheochromocytoma: experience in 400 cases. J Nucl Med. 1985 Jun;26(6):576–585. [PubMed] [Google Scholar]
  24. Smets L. A., Loesberg C., Janssen M., Metwally E. A., Huiskamp R. Active uptake and extravesicular storage of m-iodobenzylguanidine in human neuroblastoma SK-N-SH cells. Cancer Res. 1989 Jun 1;49(11):2941–2944. [PubMed] [Google Scholar]
  25. Soule H. D., Vazguez J., Long A., Albert S., Brennan M. A human cell line from a pleural effusion derived from a breast carcinoma. J Natl Cancer Inst. 1973 Nov;51(5):1409–1416. doi: 10.1093/jnci/51.5.1409. [DOI] [PubMed] [Google Scholar]
  26. Tumilowicz J. J., Nichols W. W., Cholon J. J., Greene A. E. Definition of a continuous human cell line derived from neuroblastoma. Cancer Res. 1970 Aug;30(8):2110–2118. [PubMed] [Google Scholar]
  27. Vaidyanathan G., Zalutsky M. R. No-carrier-added synthesis of meta-[131I]iodobenzylguanidine. Appl Radiat Isot. 1993 Mar;44(3):621–628. doi: 10.1016/0969-8043(93)90179-e. [DOI] [PubMed] [Google Scholar]
  28. Voûte P. A., Hoefnagel C. A., de Kraker J., Valdes Olmos R., Bakker D. J., van de Kleij A. J. Results of treatment with 131 I-metaiodobenzylguanidine (131 I-MIBG) in patients with neuroblastoma. Future prospects of zetotherapy. Prog Clin Biol Res. 1991;366:439–445. [PubMed] [Google Scholar]

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