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. 1994 Nov;70(5):873–879. doi: 10.1038/bjc.1994.413

Laser-induced fluorescence in malignant and normal tissue in mice injected with two different carotenoporphyrins.

H Nilsson 1, J Johansson 1, K Svanberg 1, S Svanberg 1, G Jori 1, E Reddi 1, A Segalla 1, D Gust 1, A L Moore 1, T A Moore 1
PMCID: PMC2033524  PMID: 7947092

Abstract

Laser-induced fluorescence (LIF) was used to characterise the localisation of an intravenously administered trimethylated carotenoporphyrin [CP(Me)3] and a trimethoxylated carotenoporphyrin [CP(OMe)3] in an intramuscularly transplanted malignant tumour (MS-2 fibrosarcoma) and healthy muscle in female Balb/c mice, 3, 24, 48 and 96 h post injection. The fluorescence was induced with a dye laser pumped by a nitrogen laser, emitting light at 425 nm. The fluorescence spectra were recorded in the region 455-760 nm using a polychromator equipped with an image-intensified CCD camera. The tumour/peritumoral muscle ratio was about 5:1 for CP(Me)3 and about 6:1 for CP(OMe)3 in terms of the background-free fluorescence intensity, which peaked at about 655 nm. By including the endogenous tissue fluorescence, the contrast was further enhanced by a factor of approximately 2.

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Selected References

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