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. 1995 Feb;71(2):311–316. doi: 10.1038/bjc.1995.62

Radiation-induced DNA double-strand break rejoining in human tumour cells.

M I Núñez 1, M Villalobos 1, N Olea 1, M T Valenzuela 1, V Pedraza 1, T J McMillan 1, J M Ruiz de Almodóvar 1
PMCID: PMC2033588  PMID: 7841046

Abstract

Five established human breast cancer cell lines and one established human bladder cancer cell line of varying radiosensitivity have been used to determine whether the rejoining of DNA double-strand breaks (dsbs) shows a correlation with radiosensitivity. The kinetics of dsb rejoining was biphasic and both components proceeded exponentially with time. The half-time (t1/2) of rejoining ranged from 18.0 +/- 1.4 to 36.4 +/- 3.2 min (fast rejoining process) and from 1.5 +/- 0.2 to 5.1 +/- 0.2 h (slow rejoining process). We found a statistically significant relationship between the survival fraction at 2 Gy (SF2) and the t1/2 of the fast rejoining component (r = 0.949, P = 0.0039). Our results suggest that cell lines which show rapid rejoining are more radioresistant. These results support the view that, as well as the level of damage induction that we have reported previously, the repair process is a major determinant of cellular radiosensitivity. It is possible that the differences found in DNA dsb rejoining and the differences in DNA dsb induction are related by a common mechanism, e.g. conformation of chromatin in the cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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