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. 1994 Dec;70(6):1182–1185. doi: 10.1038/bjc.1994.469

Expression of interleukin 10 in human melanoma.

S Krüger-Krasagakes 1, K Krasagakis 1, C Garbe 1, E Schmitt 1, C Hüls 1, T Blankenstein 1, T Diamantstein 1
PMCID: PMC2033698  PMID: 7981073

Abstract

The expression of interleukin 10 (IL-10) mRNA in human malignant melanoma was investigated by reverse transcriptase polymerase chain reaction analysis. Selective expression of IL-10 mRNA in tissues of primary melanomas and melanoma metastases was found in comparison with normal skin. In addition, strong expression of IL-10 mRNA and of biologically active IL-10 was detected in 3 out of 13 melanoma cell lines. Normal melanocytes consistently expressed low levels of IL-10 mRNA but did not produce detectable IL-10 protein, nor did keratinocytes or fibroblasts. The production of biologically active IL-10 by melanoma cell lines suggests that IL-10 mRNA in melanoma lesions may derive at least in part from the tumour cells themselves. Tumour-infiltrating cells, however, could also be a source of IL-10 in melanoma tissues. The presence of IL-10 in melanoma lesions may contribute to the postulated 'paralysis' of an anti-melanoma immune response.

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Selected References

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