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. 1995 Apr;71(4):814–818. doi: 10.1038/bjc.1995.157

Loss of heterozygosity occurs at the D11S29 locus on chromosome 11q23 in invasive cervical carcinoma.

P B Bethwaite 1, J Koreth 1, C S Herrington 1, J O McGee 1
PMCID: PMC2033723  PMID: 7710949

Abstract

Allelotypic detection of loss of heterozygosity (LOH) has been used to identify putative tumour-suppressor genes. Loci on human chromosome 11q23 are frequently altered in malignant disease, and LOH has been reported at an anonymous D11S29 locus at 11q23 in a proportion of breast and ovarian cancers and malignant melanomas. Previous studies have reported a high frequency of LOH in cervical carcinoma mapping to 11q23. Using polymerase chain reaction techniques employing probes for a recently described polymorphic dinucleotide microsatellite within this locus, we have searched for LOH in 69 cases of invasive cervical carcinoma. Genomic material was microdissected from sections cut from archival paraffin-embedded material, using the patients' constitutional genotype as a control Sixty-two (90%) of the cases were informative, and LOH occurred in 25/62 (40%) of tumours. Loss of an arm or single chromosome 11 is a well-recognised event in cervical carcinoma, and by employing other microsatellite polymorphisms mapping to 11q13 and 11p11-p12 we excluded those cases with widespread allelic loss. By doing so, LOH at D11S29 was found in 16/53 (30%) of tumours. The findings suggest a putative tumour-suppressor gene on 11q involved in cervical carcinogenesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkin N. B., Baker M. C., Fox M. F. Chromosome changes in 43 carcinomas of the cervix uteri. Cancer Genet Cytogenet. 1990 Feb;44(2):229–241. doi: 10.1016/0165-4608(90)90052-c. [DOI] [PubMed] [Google Scholar]
  2. Cannizzaro L. A., Dürst M., Mendez M. J., Hecht B. K., Hecht F. Regional chromosome localization of human papillomavirus integration sites near fragile sites, oncogenes, and cancer chromosome breakpoints. Cancer Genet Cytogenet. 1988 Jul 1;33(1):93–98. doi: 10.1016/0165-4608(88)90054-4. [DOI] [PubMed] [Google Scholar]
  3. Cawkwell L., Bell S. M., Lewis F. A., Dixon M. F., Taylor G. R., Quirke P. Rapid detection of allele loss in colorectal tumours using microsatellites and fluorescent DNA technology. Br J Cancer. 1993 Jun;67(6):1262–1267. doi: 10.1038/bjc.1993.236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chen T. M., Defendi V. Functional interaction of p53 with HPV18 E6, c-myc and H-ras in 3T3 cells. Oncogene. 1992 Aug;7(8):1541–1547. [PubMed] [Google Scholar]
  5. De Braekeleer M., Sreekantaiah C., Haas O. Herpes simplex virus and human papillomavirus sites correlate with chromosomal breakpoints in human cervical carcinoma. Cancer Genet Cytogenet. 1992 Apr;59(2):135–137. doi: 10.1016/0165-4608(92)90207-o. [DOI] [PubMed] [Google Scholar]
  6. Don R. H., Cox P. T., Wainwright B. J., Baker K., Mattick J. S. 'Touchdown' PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res. 1991 Jul 25;19(14):4008–4008. doi: 10.1093/nar/19.14.4008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Foulkes W. D., Campbell I. G., Stamp G. W., Trowsdale J. Loss of heterozygosity and amplification on chromosome 11q in human ovarian cancer. Br J Cancer. 1993 Feb;67(2):268–273. doi: 10.1038/bjc.1993.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hampton G. M., Penny L. A., Baergen R. N., Larson A., Brewer C., Liao S., Busby-Earle R. M., Williams A. W., Steel C. M., Bird C. C. Loss of heterozygosity in cervical carcinoma: subchromosomal localization of a putative tumor-suppressor gene to chromosome 11q22-q24. Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):6953–6957. doi: 10.1073/pnas.91.15.6953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hauge X. Y., Evans G. A., Litt M. Dinucleotide repeat polymorphism at the D11S534 locus. Nucleic Acids Res. 1991 Aug 11;19(15):4308–4308. doi: 10.1093/nar/19.15.4308-a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jones M. H., Nakamura Y. Deletion mapping of chromosome 3p in female genital tract malignancies using microsatellite polymorphisms. Oncogene. 1992 Aug;7(8):1631–1634. [PubMed] [Google Scholar]
  11. Koi M., Morita H., Yamada H., Satoh H., Barrett J. C., Oshimura M. Normal human chromosome 11 suppresses tumorigenicity of human cervical tumor cell line SiHa. Mol Carcinog. 1989;2(1):12–21. doi: 10.1002/mc.2940020103. [DOI] [PubMed] [Google Scholar]
  12. Lane S., Wells M. Human papillomaviruses, p53, and cervical neoplasia. J Pathol. 1994 Apr;172(4):299–300. doi: 10.1002/path.1711720402. [DOI] [PubMed] [Google Scholar]
  13. Matlashewski G., Osborn K., Banks L., Stanley M., Crawford L. Transformation of primary human fibroblast cells with human papillomavirus type 16 DNA and EJ-ras. Int J Cancer. 1988 Aug 15;42(2):232–238. doi: 10.1002/ijc.2910420215. [DOI] [PubMed] [Google Scholar]
  14. Nordenskjöld M. Recessive mutations in the oncogenesis of certain solid tumors. Immunol Ser. 1990;51:145–167. [PubMed] [Google Scholar]
  15. Orphanos V., McGown G., Boyle J. M., Santibanez-Koref M. Thirteen dinucleotide repeat polymorphisms on chromosome 6. Hum Mol Genet. 1993 Dec;2(12):2196–2196. doi: 10.1093/hmg/2.12.2196. [DOI] [PubMed] [Google Scholar]
  16. Pan L. X., Diss T. C., Peng H. Z., Isaacson P. G. Clonality analysis of defined B-cell populations in archival tissue sections using microdissection and the polymerase chain reaction. Histopathology. 1994 Apr;24(4):323–327. doi: 10.1111/j.1365-2559.1994.tb00532.x. [DOI] [PubMed] [Google Scholar]
  17. Rowley J. D. Rearrangements involving chromosome band 11Q23 in acute leukaemia. Semin Cancer Biol. 1993 Dec;4(6):377–385. [PubMed] [Google Scholar]
  18. Russell S. E., Lowry W. S., Atkinson R. J., Hickey I. Homozygosity of the short arm of chromosome 17 in cervical carcinoma. Cancer Lett. 1992 Apr 30;63(3):243–247. doi: 10.1016/0304-3835(92)90267-y. [DOI] [PubMed] [Google Scholar]
  19. Saxon P. J., Srivatsan E. S., Stanbridge E. J. Introduction of human chromosome 11 via microcell transfer controls tumorigenic expression of HeLa cells. EMBO J. 1986 Dec 20;5(13):3461–3466. doi: 10.1002/j.1460-2075.1986.tb04670.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Solomon E., Borrow J., Goddard A. D. Chromosome aberrations and cancer. Science. 1991 Nov 22;254(5035):1153–1160. doi: 10.1126/science.1957167. [DOI] [PubMed] [Google Scholar]
  21. Sreekantaiah C., De Braekeleer M., Haas O. Cytogenetic findings in cervical carcinoma. A statistical approach. Cancer Genet Cytogenet. 1991 May;53(1):75–81. doi: 10.1016/0165-4608(91)90116-c. [DOI] [PubMed] [Google Scholar]
  22. Srivatsan E. S., Misra B. C., Venugopalan M., Wilczynski S. P. Loss of heterozygosity for alleles on chromosome II in cervical carcinoma. Am J Hum Genet. 1991 Oct;49(4):868–877. [PMC free article] [PubMed] [Google Scholar]
  23. Tomlinson I. P., Gammack A. J., Stickland J. E., Mann G. J., MacKie R. M., Kefford R. F., McGee J. O. Loss of heterozygosity in malignant melanoma at loci on chromosome 11 and 17 implicated in the pathogenesis of other cancers. Genes Chromosomes Cancer. 1993 Jul;7(3):169–172. doi: 10.1002/gcc.2870070310. [DOI] [PubMed] [Google Scholar]
  24. Warnich L., Groenewald I., Theart L., Retief A. E. Highly informative dinucleotide repeat polymorphism at the D11S29 locus on chromosome 11q23. Hum Genet. 1992 May;89(3):357–359. doi: 10.1007/BF00220560. [DOI] [PubMed] [Google Scholar]
  25. Whetsell L., Maw G., Nadon N., Ringer D. P., Schaefer F. V. Polymerase chain reaction microanalysis of tumors from stained histological slides. Oncogene. 1992 Nov;7(11):2355–2361. [PubMed] [Google Scholar]
  26. Yokota J., Tsukada Y., Nakajima T., Gotoh M., Shimosato Y., Mori N., Tsunokawa Y., Sugimura T., Terada M. Loss of heterozygosity on the short arm of chromosome 3 in carcinoma of the uterine cervix. Cancer Res. 1989 Jul 1;49(13):3598–3601. [PubMed] [Google Scholar]

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