Abstract
Methanosarcina barkeri 227 and Methanosarcina mazei S-6 grew with acetate as the substrate; we found little effect of H2 on the rate of aceticlastic growth in the presence of various H2 pressures between 2 and 810 Pa. We used physical (H2 addition or flushing the headspace to remove H2) and biological (H2-producing or -utilizing bacteria in cocultures) methods for controlling H2 pressure in Methanosarcina cultures growing on acetate. Added H2 (ca. 100 Pa) was removed rapidly (a few hours) by M. barkeri and slowly (within a day) by M. mazei. When the H2 produced by the aceticlastic methanogens was removed by coculturing with an H2-using Desulfovibrio sp., the H2 pressure was about 2.2 Pa. Under these conditions the stoichiometry of aceticlastic methanogenesis did not change. H2-grown inocula of M. barkeri grew with acetate as the sole catabolic substrate if the inoculum culture was transferred during logarithmic growth to acetate-containing medium or if the transfer was accomplished within 1 or 2 days after exhaustion of H2. H2-grown cultures incubated for 4 or more days after exhaustion of H2 were able to grow with H2 but not with acetate as the sole catabolic substrate. Addition of small quantities of H2 to acetate-containing medium permitted these cultures to initiate growth on acetate.
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