Abstract
UV absorption by aflatoxins was monitored in GY agar medium by UV photography. In the UV photographs, aflatoxin-producing molds were identified as gray or black colonies, whereas aflatoxin-nonproducing molds appeared as white colonies. By cellophane transplantation experiments and silica gel thin-layer chromatography, the products absorbing UV light substantially were found to be mainly aflatoxins B1 and G1 excreted from the mold mycelium into the agar medium. UV absorption did not occur when the agar medium contained aflatoxin-noninducible carbon sources instead of glucose. Various inhibitors of aflatoxin production, such as dichlorovos and dimethyl sulfoxide, also decreased the intensity of UV absorption. These results indicate that this technique can be used as a simple, safe, and rapid method of screening aflatoxin-producing molds.
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- Bennett J. W., Christensen S. B. New perspectives on aflatoxin biosynthesis. Adv Appl Microbiol. 1983;29:53–92. doi: 10.1016/s0065-2164(08)70354-x. [DOI] [PubMed] [Google Scholar]
- Bennett J. W., Goldblatt L. A. The isolation of mutants of Aspergillus flavus and A.parasiticus with altered aflatoxin producing ability. Sabouraudia. 1973 Nov;11(3):235–241. [PubMed] [Google Scholar]
- Buxton F. P., Gwynne D. I., Davies R. W. Transformation of Aspergillus niger using the argB gene of Aspergillus nidulans. Gene. 1985;37(1-3):207–214. doi: 10.1016/0378-1119(85)90274-4. [DOI] [PubMed] [Google Scholar]
- Davis N. D., Diener U. L. Growth and aflatoxin production by Aspergillus parasiticus from various carbon sources. Appl Microbiol. 1968 Jan;16(1):158–159. doi: 10.1128/am.16.1.158-159.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davis N. D., Diener U. L. Inhibition of Aflatoxin Synthesis by p-Aminobenzoic Acid, Potassium Sulfite, and Potassium Fluoride. Appl Microbiol. 1967 Nov;15(6):1517–1518. doi: 10.1128/am.15.6.1517-1518.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Draughon F. A., Ayres J. C. Inhibition of aflatoxin production by selected insecticides. Appl Environ Microbiol. 1981 Apr;41(4):972–976. doi: 10.1128/aem.41.4.972-976.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fitzell D. L., Singh R., Hsieh D. P., Motell E. L. Nuclear magnetic resonance identification of versiconal hemiacetal acetate as an intermediate in aflatoxin biosynthesis. J Agric Food Chem. 1977 Sep-Oct;25(5):1193–1197. doi: 10.1021/jf60213a024. [DOI] [PubMed] [Google Scholar]
- Gupta S. R., Prasanna H. R., Viswanathan L., Venkitasubramanian T. A. Synthesis of aflatoxins by the non-growing mycelia of Aspergillus parasiticus and the effect of inhibitors. J Gen Microbiol. 1975 Dec;91(2):417–420. doi: 10.1099/00221287-91-2-417. [DOI] [PubMed] [Google Scholar]
- Hara S., Fennell D. I., Hesseltine C. W. Aflatoxin-producing strains of Aspergillus flavus detected by fluorescence of agar medium under ultraviolet light. Appl Microbiol. 1974 Jun;27(6):1118–1123. doi: 10.1128/am.27.6.1118-1123.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lillard H. S., Hanlin R. T., Lillard D. A. Aflatoxigenic isolates of Aspergillus flavus from pecans. Appl Microbiol. 1970 Jan;19(1):128–130. doi: 10.1128/am.19.1.128-130.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stewart R. G., Wyatt R. D., Ashmore M. D. The effect of various antifungal agents on aflatoxin production and growth characteristics of Aspergillus parasiticus and Aspergillus flavus in liquid medium. Poult Sci. 1977 Sep;56(5):1630–1635. doi: 10.3382/ps.0561630. [DOI] [PubMed] [Google Scholar]
- de Vogel P., van Rhee R., Blanche Koelensmid W. A. A rapid screening test for aflatoxin-synthesizing aspergilli of the flavus-oryzae group. J Appl Bacteriol. 1965 Aug;28(2):213–220. doi: 10.1111/j.1365-2672.1965.tb02145.x. [DOI] [PubMed] [Google Scholar]