Abstract
Nine bacterial strains growing on inulin as the sole carbon and energy source were isolated from soil samples by enrichment culture on a mineral medium. Four of the strains were thermophilic and belong to the genus Bacillus. The thermophilic strains synthesized a β-fructosidase that was active on both inulin and sucrose. The presence of inulin in the culture medium is necessary for enzyme synthesis. Most of the activity on inulin was recovered in the culture medium, and the enzyme was synthesized during cell growth.
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- Allais J. J., Kammoun S., Blanc P., Girard C., Baratti J. C. Isolation and characterization of bacterial strains with inulinase activity. Appl Environ Microbiol. 1986 Nov;52(5):1086–1090. doi: 10.1128/aem.52.5.1086-1090.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cooney C. L., Levine D. W. Microbial utilization of methanol. Adv Appl Microbiol. 1972;15:337–365. doi: 10.1016/s0065-2164(08)70096-0. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Margaritis A., Bajpai P. Effect of Sugar Concentration in Jerusalem Artichoke Extract on Kluyveromyces marxianus Growth and Ethanol Production. Appl Environ Microbiol. 1983 Feb;45(2):723–725. doi: 10.1128/aem.45.2.723-725.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SMOGYI M. Notes on sugar determination. J Biol Chem. 1952 Mar;195(1):19–23. [PubMed] [Google Scholar]
- Sattler L., Zerban F. W. The Dreywood Anthrone Reaction as Affected by Carbohydrate Structure. Science. 1948 Aug 27;108(2800):207–207. doi: 10.1126/science.108.2800.207. [DOI] [PubMed] [Google Scholar]
- Tanaka K., Uchiyama T., Ito A. Formation of di-D-fructofuranose I,2':2,3' dianhydride from inulin by an extracellular inulase of Arthrobacter ureafaciens. Biochim Biophys Acta. 1972 Sep 19;284(1):248–256. doi: 10.1016/0005-2744(72)90063-0. [DOI] [PubMed] [Google Scholar]
- Uchiyama T. Action of Arthrobacter ureafaciens inulinase II on several oligofructans and bacterial levans. Biochim Biophys Acta. 1975 Jul 27;397(1):153–163. doi: 10.1016/0005-2744(75)90189-8. [DOI] [PubMed] [Google Scholar]
- Vandamme E. J., Derycke D. G. Microbial inulinases: fermentation process, properties, and applications. Adv Appl Microbiol. 1983;29:139–176. doi: 10.1016/s0065-2164(08)70356-3. [DOI] [PubMed] [Google Scholar]