Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1987 May;53(5):946–948. doi: 10.1128/aem.53.5.946-948.1987

H2-CO2 Recirculation and pH Control for Growth of Methanogens in Mass Culture

David R Boone 1,*, Indra M Mathrani 1, Robert A Mah 1
PMCID: PMC203791  PMID: 16347354

Abstract

We modified a fermentor (10-liter liquid volume) for the growth of anaerobic, H2-CO2-catabolizing bacteria. Gas in the fermentor (ca. 10% CO2, 50% H2, 40% CH4) was recirculated by a diaphragm pump. During growth, the gas composition was maintained by the addition of a mixture of 80% H2 and 20% CO2, and this addition was controlled by a pH auxostat. During gas addition, gas was discharged from the recirculating gas stream and was collected by the displacement of an acidified salt solution.

Full text

PDF
948

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. TRUEPER H. G., SCHLEGEL H. G. SULPHUR METABOLISM IN THIORHODACEAE. I. QUANTITATIVE MEASUREMENTS ON GROWING CELLS OF CHROMATIUM OKENII. Antonie Van Leeuwenhoek. 1964;30:225–238. doi: 10.1007/BF02046728. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES