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. 1987 Jul;53(7):1705–1707. doi: 10.1128/aem.53.7.1705-1707.1987

Identification of Lotus Rhizobia by Direct DNA Hybridization of Crushed Root Nodules

J E Cooper 1,*, A J Bjourson 1, J K Thompson 1
PMCID: PMC203937  PMID: 16347400

Abstract

Hybridization of crushed Lotus pedunculatus root nodules with 32P-labeled total genomic DNA probes was used to identify Rhizobium loti and Bradyrhizobium sp. (Lotus rhizobia). Probes always hybridized with homologous target DNA and frequently with DNAs of other strains from the same genus. Intergeneric hybridization did not occur. Results were comparable to those from colony hybridization.

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Selected References

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