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. 1993 Mar;175(6):1717–1722. doi: 10.1128/jb.175.6.1717-1722.1993

Possible role of a choline-containing teichoic acid in the maintenance of normal cell shape and physiology in Streptococcus oralis.

D S Horne 1, A Tomasz 1
PMCID: PMC203966  PMID: 8449879

Abstract

Streptococcus oralis ATCC 35037 took up radioactively labeled choline from growth medium. Most of the choline (80 to 90%) was incorporated into the cell wall teichoic acid, and about 10% was localized in the plasma membrane. While cells grew in choline-free medium, they did so at slow rates and produced cell walls with greatly reduced amounts of phosphate and no detectable choline. Cells grown in choline-free medium had grossly abnormal shape and size. Both biochemical and morphological abnormalities were reversible by addition of choline to the medium.

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Selected References

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  1. Boylan R. J., Mendelson N. H., Brooks D., Young F. E. Regulation of the bacterial cell wall: analysis of a mutant of Bacillus subtilis defective in biosynthesis of teichoic acid. J Bacteriol. 1972 Apr;110(1):281–290. doi: 10.1128/jb.110.1.281-290.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Briles E. B., Tomasz A. Pneumococcal Forssman antigen. A choline-containing lipoteichoic acid. J Biol Chem. 1973 Sep 25;248(18):6394–6397. [PubMed] [Google Scholar]
  3. Brundish D. E., Baddiley J. Pneumococcal C-substance, a ribitol teichoic acid containing choline phosphate. Biochem J. 1968 Dec;110(3):573–582. doi: 10.1042/bj1100573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Forsberg C. W., Wyrick P. B., Ward J. B., Rogers H. J. Effect of phosphate limitation on the morphology and wall composition of Bacillus licheniformis and its phosphoglucomutase-deficient mutants. J Bacteriol. 1973 Feb;113(2):969–984. doi: 10.1128/jb.113.2.969-984.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Garcia-Bustos J. F., Chait B. T., Tomasz A. Structure of the peptide network of pneumococcal peptidoglycan. J Biol Chem. 1987 Nov 15;262(32):15400–15405. [PubMed] [Google Scholar]
  6. Garcia-Bustos J. F., Tomasz A. Teichoic acid-containing muropeptides from Streptococcus pneumoniae as substrates for the pneumococcal autolysin. J Bacteriol. 1987 Feb;169(2):447–453. doi: 10.1128/jb.169.2.447-453.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. García E., García J. L., Ronda C., García P., López R. Cloning and expression of the pneumococcal autolysin gene in Escherichia coli. Mol Gen Genet. 1985;201(2):225–230. doi: 10.1007/BF00425663. [DOI] [PubMed] [Google Scholar]
  8. Honeyman A. L., Stewart G. C. Identification of the protein encoded by rodC, a cell division gene from Bacillus subtilis. Mol Microbiol. 1988 Nov;2(6):735–741. doi: 10.1111/j.1365-2958.1988.tb00084.x. [DOI] [PubMed] [Google Scholar]
  9. Horne D., Tomasz A. Pneumococcal Forssman antigen: enrichment in mesosomal membranes and specific binding to the autolytic enzyme of Streptococcus pneumoniae. J Bacteriol. 1985 Jan;161(1):18–24. doi: 10.1128/jb.161.1.18-24.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jennings H. J., Lugowski C., Young N. M. Structure of the complex polysaccharide C-substance from Streptococcus pneumoniae type 1. Biochemistry. 1980 Sep 30;19(20):4712–4719. doi: 10.1021/bi00561a026. [DOI] [PubMed] [Google Scholar]
  11. Karamata D., Pooley H. M., Monod M. Expression of heterologous genes for wall teichoic acid in Bacillus subtilis 168. Mol Gen Genet. 1987 Apr;207(1):73–81. doi: 10.1007/BF00331493. [DOI] [PubMed] [Google Scholar]
  12. LACKS S., HOTCHKISS R. D. A study of the genetic material determining an enzyme in Pneumococcus. Biochim Biophys Acta. 1960 Apr 22;39:508–518. doi: 10.1016/0006-3002(60)90205-5. [DOI] [PubMed] [Google Scholar]
  13. Mendelson N. H. Bacterial growth and division: genes, structures, forces, and clocks. Microbiol Rev. 1982 Sep;46(3):341–375. doi: 10.1128/mr.46.3.341-375.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mosser J. L., Tomasz A. Choline-containing teichoic acid as a structural component of pneumococcal cell wall and its role in sensitivity to lysis by an autolytic enzyme. J Biol Chem. 1970 Jan 25;245(2):287–298. [PubMed] [Google Scholar]
  15. Schmidhuber S., Ludwig W., Schleifer K. H. Construction of a DNA probe for the specific identification of Streptococcus oralis. J Clin Microbiol. 1988 May;26(5):1042–1044. doi: 10.1128/jcm.26.5.1042-1044.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. TOMASZ A., JAMIESON J. D., OTTOLENGHI E. THE FINE STRUCTURE OF DIPLOCOCCUS PNEUMONIAE. J Cell Biol. 1964 Aug;22:453–467. doi: 10.1083/jcb.22.2.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tomasz A. Biological consequences of the replacement of choline by ethanolamine in the cell wall of Pneumococcus: chanin formation, loss of transformability, and loss of autolysis. Proc Natl Acad Sci U S A. 1968 Jan;59(1):86–93. doi: 10.1073/pnas.59.1.86. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tomasz A. Choline in the cell wall of a bacterium: novel type of polymer-linked choline in Pneumococcus. Science. 1967 Aug 11;157(3789):694–697. doi: 10.1126/science.157.3789.694. [DOI] [PubMed] [Google Scholar]
  19. Tomasz A., Westphal M., Briles E. B., Fletcher P. On the physiological functions of teichoic acids. J Supramol Struct. 1975;3(1):1–16. doi: 10.1002/jss.400030102. [DOI] [PubMed] [Google Scholar]
  20. Ward J. B. Teichoic and teichuronic acids: biosynthesis, assembly, and location. Microbiol Rev. 1981 Jun;45(2):211–243. doi: 10.1128/mr.45.2.211-243.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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