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. 1987 Aug;53(8):1775–1779. doi: 10.1128/aem.53.8.1775-1779.1987

An alkaline approach to treating cooling towers for control of Legionella pneumophila.

S J States 1, L F Conley 1, S G Towner 1, R S Wolford 1, T E Stephenson 1, A M McNamara 1, R M Wadowsky 1, R B Yee 1
PMCID: PMC203997  PMID: 3662515

Abstract

Earlier field and laboratory studies have shown that Legionella species survive and multiply in the pH range 5.5 to 9.2. Additionally, the technical feasibility of operating cooling towers at elevated alkalinities and pH has previously been documented by published guidelines. The guidelines indicate that these conditions facilitate corrosion control and favor chlorine persistence which enhances the effectiveness of continuous chlorination in biofouling control. This information suggests that control of Legionella species in cooling towers can be accomplished by operating the towers under alkaline conditions. To test this possibility, we collected water samples over a period of months from a hospital cooling tower. The samples were analyzed for a variety of chemical parameters. Subsamples were pasteurized and inoculated with non-agar-passaged Legionella pneumophila which had been maintained in tap water. Correlation of subsequent Legionella growth with corresponding pH and alkalinity values revealed statistically significant inverse associations. These data support the hypothesis that operating cooling towers outside of the optimal conditions for Legionella growth (e.g., at elevated alkalinities and a pH greater than 9) may be a useful approach to controlling growth in this habitat.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Band J. D., LaVenture M., Davis J. P., Mallison G. F., Skaliy P., Hayes P. S., Schell W. L., Weiss H., Greenberg D. J., Fraser D. W. Epidemic Legionnaires' disease. Airborne transmission down a chimney. JAMA. 1981 Jun 19;245(23):2404–2407. [PubMed] [Google Scholar]
  2. Carson L. A., Favero M. S., Bond W. W., Petersen N. J. Factors affecting comparative resistance of naturally occurring and subcultured Pseudomonas aeruginosa to disinfectants. Appl Microbiol. 1972 May;23(5):863–869. doi: 10.1128/am.23.5.863-869.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cordes L. G., Fraser D. W., Skaliy P., Perlino C. A., Elsea W. R., Mallison G. F., Hayes P. S. Legionnaires' disease outbreak at an Atlanta, Georgia, Country Club: evidence for spread from an evaporative condenser. Am J Epidemiol. 1980 Apr;111(4):425–431. doi: 10.1093/oxfordjournals.aje.a112917. [DOI] [PubMed] [Google Scholar]
  4. Dondero T. J., Jr, Rendtorff R. C., Mallison G. F., Weeks R. M., Levy J. S., Wong E. W., Schaffner W. An outbreak of Legionnaires' disease associated with a contaminated air-conditioning cooling tower. N Engl J Med. 1980 Feb 14;302(7):365–370. doi: 10.1056/NEJM198002143020703. [DOI] [PubMed] [Google Scholar]
  5. England A. C., 3rd, Fraser D. W., Mallison G. F., Mackel D. C., Skaliy P., Gorman G. W. Failure of Legionella pneumophila sensitivities to predict culture results from disinfectant-treated air-conditioning cooling towers. Appl Environ Microbiol. 1982 Jan;43(1):240–244. doi: 10.1128/aem.43.1.240-244.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Favero M. S., Carson L. A., Bond W. W., Petersen N. J. Pseudomonas aeruginosa: growth in distilled water from hospitals. Science. 1971 Aug 27;173(3999):836–838. doi: 10.1126/science.173.3999.836. [DOI] [PubMed] [Google Scholar]
  7. Fliermans C. B., Cherry W. B., Orrison L. H., Smith S. J., Tison D. L., Pope D. H. Ecological distribution of Legionella pneumophila. Appl Environ Microbiol. 1981 Jan;41(1):9–16. doi: 10.1128/aem.41.1.9-16.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fliermans C. B., Harvey R. S. Effectiveness of 1-bromo-3-chloro-5,5-dimethylhydantoin against Legionella pneumophila in a cooling tower. Appl Environ Microbiol. 1984 Jun;47(6):1307–1310. doi: 10.1128/aem.47.6.1307-1310.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Glick T. H., Gregg M. B., Berman B., Mallison G., Rhodes W. W., Jr, Kassanoff I. Pontiac fever. An epidemic of unknown etiology in a health department: I. Clinical and epidemiologic aspects. Am J Epidemiol. 1978 Feb;107(2):149–160. doi: 10.1093/oxfordjournals.aje.a112517. [DOI] [PubMed] [Google Scholar]
  10. Gordon A. S., Millero F. J. Electrolyte effects on attachment of an estuarine bacterium. Appl Environ Microbiol. 1984 Mar;47(3):495–499. doi: 10.1128/aem.47.3.495-499.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gorman G. W., Feeley J. C., Steigerwalt A., Edelstein P. H., Moss C. W., Brenner D. J. Legionella anisa: a new species of Legionella isolated from potable waters and a cooling tower. Appl Environ Microbiol. 1985 Feb;49(2):305–309. doi: 10.1128/aem.49.2.305-309.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Grace R. D., Dewar N. E., Barnes W. G., Hodges G. R. Susceptibility of Legionella pneumophila to three cooling tower microbicides. Appl Environ Microbiol. 1981 Jan;41(1):233–236. doi: 10.1128/aem.41.1.233-236.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kuchta J. M., States S. J., McGlaughlin J. E., Overmeyer J. H., Wadowsky R. M., McNamara A. M., Wolford R. S., Yee R. B. Enhanced chlorine resistance of tap water-adapted Legionella pneumophila as compared with agar medium-passaged strains. Appl Environ Microbiol. 1985 Jul;50(1):21–26. doi: 10.1128/aem.50.1.21-26.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kuchta J. M., States S. J., McNamara A. M., Wadowsky R. M., Yee R. B. Susceptibility of Legionella pneumophila to chlorine in tap water. Appl Environ Microbiol. 1983 Nov;46(5):1134–1139. doi: 10.1128/aem.46.5.1134-1139.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kurtz J. B., Bartlett C. L., Newton U. A., White R. A., Jones N. L. Legionella pneumophila in cooling water systems. Report of a survey of cooling towers in London and a pilot trial of selected biocides. J Hyg (Lond) 1982 Jun;88(3):369–381. doi: 10.1017/s0022172400070248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Miller R. P. Cooling towers and evaporative condensers. Ann Intern Med. 1979 Apr;90(4):667–670. doi: 10.7326/0003-4819-90-4-667. [DOI] [PubMed] [Google Scholar]
  17. Morris G. K., Patton C. M., Feeley J. C., Johnson S. E., Gorman G., Martin W. T., Skaliy P., Mallison G. F., Politi B. D., Mackel D. C. Isolation of the Legionnaires' disease bacterium from environmental samples. Ann Intern Med. 1979 Apr;90(4):664–666. doi: 10.7326/0003-4819-90-4-664. [DOI] [PubMed] [Google Scholar]
  18. Orrison L. H., Cherry W. B., Milan D. Isolation of Legionella pneumophilia from cooling tower water by filtration. Appl Environ Microbiol. 1981 May;41(5):1202–1205. doi: 10.1128/aem.41.5.1202-1205.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Orrison L. H., Cherry W. B., Tyndall R. L., Fliermans C. B., Gough S. B., Lambert M. A., McDougal L. K., Bibb W. F., Brenner D. J. Legionella oakridgensis: unusual new species isolated from cooling tower water. Appl Environ Microbiol. 1983 Feb;45(2):536–545. doi: 10.1128/aem.45.2.536-545.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Skaliy P., Thompson T. A., Gorman G. W., Morris G. K., McEachern H. V., Mackel D. C. Laboratory studies of disinfectants against Legionella pneumophila. Appl Environ Microbiol. 1980 Oct;40(4):697–700. doi: 10.1128/aem.40.4.697-700.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. States S. J., Conley L. F., Ceraso M., Stephenson T. E., Wolford R. S., Wadowsky R. M., McNamara A. M., Yee R. B. Effects of metals on Legionella pneumophila growth in drinking water plumbing systems. Appl Environ Microbiol. 1985 Nov;50(5):1149–1154. doi: 10.1128/aem.50.5.1149-1154.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. States S. J., Conley L. F., Kuchta J. M., Oleck B. M., Lipovich M. J., Wolford R. S., Wadowsky R. M., McNamara A. M., Sykora J. L., Keleti G. Survival and multiplication of Legionella pneumophila in municipal drinking water systems. Appl Environ Microbiol. 1987 May;53(5):979–986. doi: 10.1128/aem.53.5.979-986.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wadowsky R. M., Wolford R., McNamara A. M., Yee R. B. Effect of temperature, pH, and oxygen level on the multiplication of naturally occurring Legionella pneumophila in potable water. Appl Environ Microbiol. 1985 May;49(5):1197–1205. doi: 10.1128/aem.49.5.1197-1205.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wadowsky R. M., Yee R. B. Glycine-containing selective medium for isolation of Legionellaceae from environmental specimens. Appl Environ Microbiol. 1981 Nov;42(5):768–772. doi: 10.1128/aem.42.5.768-772.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wadowsky R. M., Yee R. B., Mezmar L., Wing E. J., Dowling J. N. Hot water systems as sources of Legionella pneumophila in hospital and nonhospital plumbing fixtures. Appl Environ Microbiol. 1982 May;43(5):1104–1110. doi: 10.1128/aem.43.5.1104-1110.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wang W. L., Blaser M. J., Cravens J., Johnson M. A. Growth, survival, and resistance of the Legionnaires' disease bacterium. Ann Intern Med. 1979 Apr;90(4):614–618. doi: 10.7326/0003-4819-90-4-614. [DOI] [PubMed] [Google Scholar]

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