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. 1987 Aug;53(8):1785–1789. doi: 10.1128/aem.53.8.1785-1789.1987

Serological Relatedness of Rhizobium fredii to Other Rhizobia and to the Bradyrhizobia

Michael J Sadowsky 1,*, B Ben Bohlool 1,†, Harold H Keyser 1
PMCID: PMC204000  PMID: 16347404

Abstract

Several isolates of Rhizobium fredii were examined for their serological relatedness to each other, to Bradyrhizobium japonicum, and to other fast- and slow-growing rhizobia. Immunofluorescence, agglutination, and immunodiffusion analyses indicated that R. fredii contains at least three separate somatic serogroups, USDA 192, USDA 194, and USDA 205. There was no cross-reaction between any of the R. fredii isolates and 13 of the 14 B. japonicum somatic serogroups tested. Cross-reactions were obtained with antisera from R. fredii and serogroup 122 of B. japonicum, Rhizobium meliloti, and several fast-growing Rhizobium spp. for Leucaena, Sesbania, and Lablab species. The serological relationship between R. fredii and R. meliloti was examined in more detail, and of 23 R. meliloti strains examined, 8 shared somatic antigens with the type strains from all three R. fredii serogroups. The serological relatedness of R. fredii to B. japonicum and R. meliloti appears to be unique since the strains are known to be biochemically and genetically diverse.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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