Abstract
Polymorphonuclear leucocytes (PMN) were harvested from the site of a non-specific acute inflammatory reaction. When cultured in vitro for 24 h the cells liberated into the supernatant factors which could enhance the proliferation of normal macrophages in the absence and presence of the mitogen phytohaemagglutinin (PHA). Similar activity was found in lysates of these cells. Using the technique of ultrafiltration it was shown that the mitogenic factors present in both PMN supernatants and lysates were under 10 000 daltons molecular weight. In addition, the chemiluminescent responses of normal macrophages were also enhanced by PMN supernatant, lysate and their respective ultrafiltrates. Our results suggest that the PMNs, which are the most abundant cell type during acute inflammation, both contain and are able to liberate low-molecular-weight macrophage-stimulatory factors which may be important in our understanding of the pathogenesis of inflammation.
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Selected References
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- Adolphe M., Fontagne J., Pelletier M., Giroud J. P. Induction of DNA synthesis in rat macrophages in vitro by inflammatory exudate. Nature. 1975 Feb 20;253(5493):637–637. doi: 10.1038/253637a0. [DOI] [PubMed] [Google Scholar]
- Adolphe M., Fontagné J., Pelletier M., Giroud J. P., Timsit J., Lechat P. Induction of DNA synthesis in rat peritoneal macrophages in culture by a pleural inflammatory exudate. Agents Actions. 1976 Feb;6(1-3):114–122. doi: 10.1007/BF01972194. [DOI] [PubMed] [Google Scholar]
- Chapes S. K., Haskill S. Evidence for granulocyte-mediated macrophage activation after C. parvum immunization. Cell Immunol. 1983 Feb 1;75(2):367–377. doi: 10.1016/0008-8749(83)90334-9. [DOI] [PubMed] [Google Scholar]
- Harris P. J. Neutrophil product with lymphocyte activating factor activity. Clin Exp Immunol. 1982 Dec;50(3):474–478. [PMC free article] [PubMed] [Google Scholar]
- Newman S. L., Henson J. E., Henson P. M. Phagocytosis of senescent neutrophils by human monocyte-derived macrophages and rabbit inflammatory macrophages. J Exp Med. 1982 Aug 1;156(2):430–442. doi: 10.1084/jem.156.2.430. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yoshinaga M., Nishime K., Nakamura S., Goto F. A PMN-derived factor that enhances DNA-synthesis in PHA or antigen-stimulated lymphocytes. J Immunol. 1980 Jan;124(1):94–99. [PubMed] [Google Scholar]