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. 1987 Oct;53(10):2384–2387. doi: 10.1128/aem.53.10.2384-2387.1987

Wood Degradation by White Rot Fungi: Cytochemical Studies Using Lignin Peroxidase-Immunoglobulin-Gold Complexes

Susana Garcia 1,*, Jean Paul Latge 1, Marie Christine Prevost 1, Matti Leisola 1
PMCID: PMC204117  PMID: 16347459

Abstract

Using an anti-lignin peroxidase antiserum-protein A-gold complex, we found lignin peroxidase mainly intracellularly in several white rot fungi colonizing sawdust under laboratory conditions. This enzyme was also present in fungi found in naturally decayed wood. However, in all cases, lignin peroxidase was located mainly inside the fungal cells. Labeled lignin peroxidase did not bind to the lignocellulosic samples tested, with the exception of poplar milled-wood lignin. These results are discussed in relation to the role of lignin peroxidase during wood degradation.

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Selected References

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  1. Bendayan M. Electron microscopical localization of nucleic acids by means of nuclease--gold complexes. Histochem J. 1981 Sep;13(5):699–710. doi: 10.1007/BF01003282. [DOI] [PubMed] [Google Scholar]
  2. Blanchette R. A., Reid I. D. Ultrastructural Aspects of Wood Delignification by Phlebia (Merulius) tremellosus. Appl Environ Microbiol. 1986 Aug;52(2):239–245. doi: 10.1128/aem.52.2.239-245.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. LAURELL C. B. ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS. Anal Biochem. 1965 Feb;10:358–361. doi: 10.1016/0003-2697(65)90278-2. [DOI] [PubMed] [Google Scholar]
  4. Ruel K., Joseleau J. P. Use of enzyme-gold complexes for the ultrastructural localization of hemicelluloses in the plant cell wall. Histochemistry. 1984;81(6):573–580. doi: 10.1007/BF00489537. [DOI] [PubMed] [Google Scholar]
  5. Takamiya H., Vogt A., Batsford S., Kuttin E. S., Müller J. Further studies on the immunoelectronmicroscopic localization of polysaccharide antigens on ultra-thin sections of Candida albicans. Mykosen. 1985 Jan;28(1):17–32. doi: 10.1111/j.1439-0507.1985.tb02069.x. [DOI] [PubMed] [Google Scholar]
  6. Tien M., Kirk T. K. Lignin-degrading enzyme from Phanerochaete chrysosporium: Purification, characterization, and catalytic properties of a unique H(2)O(2)-requiring oxygenase. Proc Natl Acad Sci U S A. 1984 Apr;81(8):2280–2284. doi: 10.1073/pnas.81.8.2280. [DOI] [PMC free article] [PubMed] [Google Scholar]

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