Abstract
Two experiments were devised to produce an experimental enteropathy. In Experiment I, male Alderley Park rats were dosed daily by gavage with 20 mg/kg and 60 mg/kg of an antibacterial compound ICI 17,363. Animals were killed sequentially at daily intervals up to and including Day 9 to study the development of the enteropathy. In Experiment II rats were dosed daily with 60 mg/kg of the same compound. All animals were killed on Day 5 owing to a rapid development of the enteropathic condition. The duodenum was examined histologically and histochemically. Duodenal changes included vacuolation of columnar epithelial cells and villus stunting. There were marked reductions in mitotic activity in the crypt epithelial cells from Day 7 onwards (Experiment I) and almost total loss of hydrolytic and oxidative enzyme activity. In Experiment II the changes were more severe and haemorrhage and erosion of the duodenal mucosa were observed. The development of the enteropathic lesion appears to be due largely to the antimitotic effect of the compound, although a direct toxic effect upon the intestinal mucosa cannot be ruled out.
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- Bowring N. E., May D. S. Impairment of salicylate uptake from rat small intestine following pretreatment with a folic acid antagonist. J Pharm Pharmacol. 1976 Feb;28(2):175–176. doi: 10.1111/j.2042-7158.1976.tb04125.x. [DOI] [PubMed] [Google Scholar]
- Gray J. E., Weaver R. N., Sinkula A. A., Schurr P. E., Moran J. Drug-induced enteropathy characterized by lipid in macrophages. Toxicol Appl Pharmacol. 1974 Jan;27(1):145–157. doi: 10.1016/0041-008x(74)90182-3. [DOI] [PubMed] [Google Scholar]
- MURGATROYD L. B. SOME SALIENT POINTS IN THE PREPARATION OF A NEW METHYL GREEN PYRONIN-STAIN AND ITS USE IN CYTOLOGY AND HISTOLOGY. Mikroskopie. 1964 Jan;18:285–287. [PubMed] [Google Scholar]
- McPherson J. R., Shorter R. G. Intestinal lesions associated with triparanol. A clinical and experimental study. Am J Dig Dis. 1965 Dec;10(12):1024–1033. doi: 10.1007/BF02233376. [DOI] [PubMed] [Google Scholar]
- Murgatroyd L. B. A quantitative investigation into the effect of fixation, temperature and acid strength upon the Feulgen reaction. J R Microsc Soc. 1968;88(1):133–139. doi: 10.1111/j.1365-2818.1968.tb00601.x. [DOI] [PubMed] [Google Scholar]
- NACHLAS M. M., TSOU K. C., DE SOUZA E., CHENG C. S., SELIGMAN A. M. Cytochemical demonstration of succinic dehydrogenase by the use of a new p-nitrophenyl substituted ditetrazole. J Histochem Cytochem. 1957 Jul;5(4):420–436. doi: 10.1177/5.4.420. [DOI] [PubMed] [Google Scholar]
- PADYKULA H. A., STRAUSS E. W., LADMAN A. J., GARDNER F. H. A morphologic and histochemical analysis of the human jejunal epithelium in nontropical sprue. Gastroenterology. 1961 Jun;40:735–765. [PubMed] [Google Scholar]
- Regarding the definition of tropical sprue. Gastroenterology. 1970 May;58(5):717–721. [PubMed] [Google Scholar]
- Robinson J. W., Antonioli J. A., Vannotti A. The effect of oral methotrexate on the rat intestine. Biochem Pharmacol. 1966 Oct;15(10):1479–1489. doi: 10.1016/0006-2952(66)90193-6. [DOI] [PubMed] [Google Scholar]
- VITALE J. J., ZAMCHECK N., DIGIORGIO J., HEGSTED D. M. Effects of aminopterin administration on the respiration and morphology of the gastrointestinal mucosa of rats. J Lab Clin Med. 1954 Apr;43(4):583–594. [PubMed] [Google Scholar]
- WACHSTEIN M., MEISEL E., NIEDZWIEDZ A. Histochemical demonstration of mitochondrial adenosine triphosphatase with the lead-adenosine triphosphate technique. J Histochem Cytochem. 1960 Sep;8:387–388. doi: 10.1177/8.5.387. [DOI] [PubMed] [Google Scholar]
- WOLL E., OLESON J. J. The effects of a folic acid antagonist (aminopterin) on albino rats; a study in the pathogenesis of sprue. Br J Exp Pathol. 1951 Oct;32(5):458–461. [PMC free article] [PubMed] [Google Scholar]