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British Journal of Experimental Pathology logoLink to British Journal of Experimental Pathology
. 1981 Jun;62(3):270–282.

Growth in culture of adenocarcinoma cells from the small intestine of sheep.

M Norval, K W Head, R W Else, H Hart, W A Neill
PMCID: PMC2041694  PMID: 7248169

Abstract

Explant cultures were initiated from adenocarcinoma of the small intestine in sheep and from various metastases. Several cell types grew, most being fibroblastic in nature. However, 2 cultures yielded mixed cells which arranged themselves into areas of epithelial-like cells surrounded by fibroblast-like cells and this pattern was consistent over 30 subcultures and several months of culturing. The epithelial-like cells were separated from the others by the use of a modified medium containing citrulline or by sedimentation through a bovine serum albumin solution. Various properties, including their growth rate in 5% and 0.5% serum, the absence of surface fibronectin and their ability to grow in semi-solid agar, indicated that they may represent carcinoma cells. Screening for virus production from these cells and all other explant cultures proved negative.

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Selected References

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