Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1987 Dec;53(12):2894–2896. doi: 10.1128/aem.53.12.2894-2896.1987

Recovery of a marker strain of Escherichia coli from ozonated water by membrane filtration.

G R Finch 1, M E Stiles 1, D W Smith 1
PMCID: PMC204218  PMID: 3324969

Abstract

Selective and nonselective growth media were evaluated at two incubation temperatures, 35 and 44.5 degrees C, for the recovery of a nalidixic acid-resistant marker strain of Escherichia coli ATCC 11775 by membrane filtration from ozonated 0.05 M phosphate buffer (pH 6.9). There were significantly fewer bacteria recovered with the standard m-FC agar when compared with the same growth medium prepared without bile salts and rosolic acid. This effect was particularly noticeable at the elevated incubation temperature of 44.5 degrees C. These findings are contrary to previous work which concluded that the standard American Public Health Association membrane filtration procedure is suitable for recovery of fecal coliform indicator bacteria from ozonated wastewater.

Full text

PDF
2894

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bissonnette G. K., Jezeski J. J., McFeters G. A., Stuart D. G. Evaluation of recovery methods to detect coliforms in water. Appl Environ Microbiol. 1977 Mar;33(3):590–595. doi: 10.1128/aem.33.3.590-595.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bissonnette G. K., Jezeski J. J., McFeters G. A., Stuart D. G. Influence of environmental stress on enumeration of indicator bacteria from natural waters. Appl Microbiol. 1975 Feb;29(2):186–194. doi: 10.1128/am.29.2.186-194.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Camper A. K., McFeters G. A. Chlorine injury and the enumeration of waterborne coliform bacteria. Appl Environ Microbiol. 1979 Mar;37(3):633–641. doi: 10.1128/aem.37.3.633-641.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Green B. L., Clausen E. M., Litsky W. Two-temperature membrane filter method for enumerating fecal coliform bacteria from chlorinated effluents. Appl Environ Microbiol. 1977 Jun;33(6):1259–1264. doi: 10.1128/aem.33.6.1259-1264.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lin S. D. Membrane filter method for recovery of fecal coliforms in chlorinated sewage effluents. Appl Environ Microbiol. 1976 Oct;32(4):547–552. doi: 10.1128/aem.32.4.547-552.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. McFeters G. A., Cameron S. C., LeChevallier M. W. Influence of diluents, media, and membrane filters on detection fo injured waterborne coliform bacteria. Appl Environ Microbiol. 1982 Jan;43(1):97–103. doi: 10.1128/aem.43.1.97-103.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Presswood W. G., Strong D. K. Modification of M-FC medium by eliminating rosolic acid. Appl Environ Microbiol. 1978 Jul;36(1):90–94. doi: 10.1128/aem.36.1.90-94.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rose R. E., Geldreich E. E., Litsky W. Improved membrane filter method for fecal coliform analysis. Appl Microbiol. 1975 Apr;29(4):532–536. doi: 10.1128/am.29.4.532-536.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Stuart D. G., McFeters G. A., Schillinger J. E. Membrane filter technique for the quantification of stressed fecal coliforms in the aquatic environment. Appl Environ Microbiol. 1977 Jul;34(1):42–46. doi: 10.1128/aem.34.1.42-46.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES