Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1987 Dec;53(12):2897–2902. doi: 10.1128/aem.53.12.2897-2902.1987

A Phosphate-Bond-Driven Dipeptide Transport System in Streptococcus cremoris Is Regulated by the Internal pH

A van Boven 1, W N Konings 1,*
PMCID: PMC204219  PMID: 16347504

Abstract

The uptake of amino acids and peptides by Streptococcus cremoris is mediated by different highly specific transport systems. The leucine transport system has a high affinity only for leucine, isoleucine, and valine and no affinity for leucyl-peptides. The transport system for leucyl-leucine is strongly inhibited by several dipeptides with hydrophobic, neutral, N-terminal amino acids but not by leucine. The leucyl-leucine transport system has a high affinity for dipeptides containing β-methyl groups in the side chain; the C terminus of the dipeptide affects the affinity to a much lower extent. Leucyl-leucine transport in whole cells was studied as a function of the internal pH at different external pH values in the presence and absence of nigericin. The internal pH was shown to be an important controlling factor in leucyl-leucine uptake, but the ΔpH was not involved as a driving force. At increasing external pH values, the affinity of the transport system for leucyl-leucine decreased. Uptake of leucyl-leucine was also studied in the presence of arsenate, which inhibited ATP synthesis by substrate-level phosphorylation. The rate of leucyl-leucine transport appeared to be dependent on the intracellular ATP concentrations. These results indicate that the energy for the leucyl-leucine transport is directly supplied by ATP.

Full text

PDF
2900

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Crow V. L., Thomas T. D. Arginine metabolism in lactic streptococci. J Bacteriol. 1982 Jun;150(3):1024–1032. doi: 10.1128/jb.150.3.1024-1032.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Driessen A. J., Kodde J., de Jong S., Konings W. N. Neutral amino acid transport by membrane vesicles of Streptococcus cremoris is subject to regulation by internal pH. J Bacteriol. 1987 Jun;169(6):2748–2754. doi: 10.1128/jb.169.6.2748-2754.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Driessen A. J., de Jong S., Konings W. N. Transport of branched-chain amino acids in membrane vesicles of Streptococcus cremoris. J Bacteriol. 1987 Nov;169(11):5193–5200. doi: 10.1128/jb.169.11.5193-5200.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Exterkate F. A. Location of Peptidases Outside and Inside the Membrane of Streptococcus cremoris. Appl Environ Microbiol. 1984 Jan;47(1):177–183. doi: 10.1128/aem.47.1.177-183.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Higgins C. F., Gibson M. M. Peptide transport in bacteria. Methods Enzymol. 1986;125:365–377. doi: 10.1016/s0076-6879(86)25031-4. [DOI] [PubMed] [Google Scholar]
  6. Hiles I. D., Powell L. M., Higgins C. F. Peptide transport in Salmonella typhimurium: molecular cloning and characterization of the oligopeptide permease genes. Mol Gen Genet. 1987 Jan;206(1):101–109. doi: 10.1007/BF00326543. [DOI] [PubMed] [Google Scholar]
  7. Kolstad J., Law B. A. Comparative peptide specificity of cell wall, membrane and intracellular peptidases of group N streptococci. J Appl Bacteriol. 1985 May;58(5):449–455. doi: 10.1111/j.1365-2672.1985.tb01484.x. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Law B. A., Kolstad J. Proteolytic systems in lactic acid bacteria. Antonie Van Leeuwenhoek. 1983 Sep;49(3):225–245. doi: 10.1007/BF00399500. [DOI] [PubMed] [Google Scholar]
  10. Law B. A. Peptide utilization by group N streptococci. J Gen Microbiol. 1978 Mar;105(1):113–118. doi: 10.1099/00221287-105-1-113. [DOI] [PubMed] [Google Scholar]
  11. Otto R., Lageveen R. G., Veldkamp H., Konings W. N. Lactate efflux-induced electrical potential in membrane vesicles of Streptococcus cremoris. J Bacteriol. 1982 Feb;149(2):733–738. doi: 10.1128/jb.149.2.733-738.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Payne J. W., Bell G. Direct determination of the properties of peptide transport systems in Escherichia coli, using a fluorescent-labeling procedure. J Bacteriol. 1979 Jan;137(1):447–455. doi: 10.1128/jb.137.1.447-455.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Payne J. W. Peptide transport in bacteria: methods, mutants and energy coupling. Biochem Soc Trans. 1983 Dec;11(6):794–798. doi: 10.1042/bst0110794. [DOI] [PubMed] [Google Scholar]
  14. Perry D., Gilvarg C. Spectrophotometric determination of affinities of peptides for their transport systems in Escherichia coli. J Bacteriol. 1984 Dec;160(3):943–948. doi: 10.1128/jb.160.3.943-948.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Poolman B., Hellingwerf K. J., Konings W. N. Regulation of the glutamate-glutamine transport system by intracellular pH in Streptococcus lactis. J Bacteriol. 1987 May;169(5):2272–2276. doi: 10.1128/jb.169.5.2272-2276.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Poolman B., Smid E. J., Konings W. N. Kinetic properties of a phosphate-bond-driven glutamate-glutamine transport system in Streptococcus lactis and Streptococcus cremoris. J Bacteriol. 1987 Jun;169(6):2755–2761. doi: 10.1128/jb.169.6.2755-2761.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Smith J. S., Hillier A. J., Lees G. J. The nature of the stimulation of the growth of Streptococcus lactis by yeast extract. J Dairy Res. 1975 Feb;42(1):123–138. doi: 10.1017/s0022029900015156. [DOI] [PubMed] [Google Scholar]
  18. ten Brink B., Konings W. N. Electrochemical proton gradient and lactate concentration gradient in Streptococcus cremoris cells grown in batch culture. J Bacteriol. 1982 Nov;152(2):682–686. doi: 10.1128/jb.152.2.682-686.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. van Boven A., Konings W. N. Energetics of Leucyl-Leucine Hydrolysis in Streptococcus cremoris Wg(2). Appl Environ Microbiol. 1986 Jan;51(1):95–100. doi: 10.1128/aem.51.1.95-100.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES