Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1979 Apr;95(1):67–80.

Pathogenesis of reactivated latent murine cytomegalovirus infection.

J D Shanley, M C Jordan, M L Cook, J G Stevens
PMCID: PMC2042281  PMID: 219700

Abstract

Sixteen weeks after inoculation, murine cytomegalovirus (MCMV) can no longer be detected in the tissues of mice. However, a 2-week course of immunosuppression with antilymphocyte serum and cortisone acetate results in reactivation and dissemination of the latent virus in all animals. In this study of reactivation, MCMV was first detected in the liver, usually during the first week of immunosuppression, and virus replication was shown to be restricted to hepatocytes. Subsequently, a viremia occurred, with spread of infection to other organs. The highest titers of virus were reached in salivary glands in which replication occurred in serous acinar cells. In the lung, virus-specific abnormalities were difficult to detect because of superimposed bacterial and fungal infections. However, interstitial pneumonitis could be produced when cortisone acetate was deleted from the immunosuppressive regimen. Although the site of virus latency has not been defined, this model system will be useful for study of reactivation of latent cytomegalovirus infection.

Full text

PDF
79

Images in this article

80Figure 3
on p.80
80Figure 4
on p.80
79Figure 1
on p.79
79Figure 2
on p.79

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BODEY G. P., WERTLAKE P. T., DOUGLAS G., LEVIN R. H. CYTOMEGALIC INCLUSION DISEASE IN PATIENTS WITH ACUTE LEUKEMIA. Ann Intern Med. 1965 May;62:899–906. doi: 10.7326/0003-4819-62-5-899. [DOI] [PubMed] [Google Scholar]
  2. BRODSKY I., ROWE W. P. Chronic subclinical infection with mouse salivary gland virus. Proc Soc Exp Biol Med. 1958 Dec;99(3):654–655. doi: 10.3181/00379727-99-24451. [DOI] [PubMed] [Google Scholar]
  3. Brody A. R., Craighead J. E. Pathogenesis of pulmonary cytomegalovirus infection in immunosuppressed mice. J Infect Dis. 1974 Jun;129(6):677–689. doi: 10.1093/infdis/129.6.677. [DOI] [PubMed] [Google Scholar]
  4. Craighead J. E. Cytomegalovirus pulmonary disease. Pathobiol Annu. 1975;5:197–220. [PubMed] [Google Scholar]
  5. Gardner M. B., Officer J. E., Parker J., Estes J. D., Rongey R. W. Induction of disseminated virulent cytomegalovirus infection by immunosuppression of naturally chronically infected wild mice. Infect Immun. 1974 Oct;10(4):966–969. doi: 10.1128/iai.10.4.966-969.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Henson D., Siegel S. E., Fuccillo D. A., Matthew E., Levine A. S. Cytomegalovirus infections during acute childhood leukemia. J Infect Dis. 1972 Nov;126(5):469–481. doi: 10.1093/infdis/126.5.469. [DOI] [PubMed] [Google Scholar]
  7. Henson D., Smith R. D., Gehrke J., Neapolitan C. Effect of cortisone on nonfatal mouse cytomegalovirus infection. Am J Pathol. 1967 Dec;51(6):1001–1011. [PMC free article] [PubMed] [Google Scholar]
  8. Henson D., Smith R. D., Gehrke J. Non-fatal mouse cytomegalovirus hepatitis. Combined morphologic, virologic and immunologic observations. Am J Pathol. 1966 Nov;49(5):871–888. [PMC free article] [PubMed] [Google Scholar]
  9. Lang D. J., Cheung K. S., Schwartz J. N., Daniels C. A., Harwood S. E. Cytomegalovirus replication and the host immune response. Yale J Biol Med. 1976 Mar;49(1):45–58. [PMC free article] [PubMed] [Google Scholar]
  10. Lang D. J. Cytomegalovirus infections in organ transplantation and post transfusion. An hypothesis. Arch Gesamte Virusforsch. 1972;37(4):365–377. doi: 10.1007/BF01241460. [DOI] [PubMed] [Google Scholar]
  11. MANNINI A., MEDEARIS D. N., Jr Mouse salivary gland virus infections. Am J Hyg. 1961 May;73:329–343. doi: 10.1093/oxfordjournals.aje.a120192. [DOI] [PubMed] [Google Scholar]
  12. Mayo D. R., Armstrong J. A., Ho M. Reactivation of murine cytomegalovirus by cyclophosphamide. Nature. 1977 Jun 23;267(5613):721–723. doi: 10.1038/267721a0. [DOI] [PubMed] [Google Scholar]
  13. Myers J. D., Spencer H. C., Jr, Watts J. C., Gregg M. B., Stewart J. A., Troupin R. H., Thomas E. D. Cytomegalovirus pneumonia after human marrow transplantation. Ann Intern Med. 1975 Feb;82(2):181–188. doi: 10.7326/0003-4819-82-2-181. [DOI] [PubMed] [Google Scholar]
  14. Olding L. B., Jensen F. C., Oldstone M. B. Pathogenesis of of cytomegalovirus infection. I. Activation of virus from bone marrow-derived lymphocytes by in vitro allogenic reaction. J Exp Med. 1975 Mar 1;141(3):561–572. doi: 10.1084/jem.141.3.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rifkind D., Goodman N., Hill R. B., Jr The clinical significance of cytomegalovirus infection in renal transplant recipients. Ann Intern Med. 1967 Jun;66(6):1116–1128. doi: 10.7326/0003-4819-66-6-1116. [DOI] [PubMed] [Google Scholar]
  16. Selgrade M. K., Osborn J. E. Role of macrophages in resistance to murine cytomegalovirus. Infect Immun. 1974 Dec;10(6):1383–1390. doi: 10.1128/iai.10.6.1383-1390.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Weller T. H. The cytomegaloviruses: ubiquitous agents with protean clinical manifestations. II. N Engl J Med. 1971 Jul 29;285(5):267–274. doi: 10.1056/NEJM197107292850507. [DOI] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES