Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1979 May;95(2):445–462.

Ultrastructural studies of the gray platelet syndrome.

J G White
PMCID: PMC2042344  PMID: 453324

Abstract

The gray platelet syndrome (GPS) is a rare inherited disorder in which peripheral blood platelets are relatively large, vacuolated, and almost devoid of cytoplasmic granulation. In the present study we have evaluated the ultrastructure and cytochemistry of platelets from 2 patients with the GPS to determine precisely which organelles are missing from their cells. The findings indicate that gray platelets contain normal numbers of mitochondria, dense bodies, peroxisomes, and lysosomes but specifically lack alpha-granules. Preliminary studies of megakaryocytes from 1 of the 2 patients suggest that the defect in granule formation may lie at the level of the Golgi zone.

Full text

PDF
446

Images in this article

460Figure 15
on p.460
460Figure 16
on p.460
460Figures 17 and 18
on p.460
460Figures 19 and 20
on p.460
458Figure 3
on p.458
458Figure 4
on p.458
458Figures 5 and 6
on p.458
458Figures 7 and 8
on p.458
462Figure 27
on p.462
462Figure 28
on p.462
462Figure 29
on p.462
462Figure 30
on p.462
462Figure 31
on p.462
462Figure 32
on p.462
459Figure 9
on p.459
459Figure 10
on p.459
459Figure 11
on p.459
459Figure 12
on p.459
459Figures 13 and 14
on p.459
461Figures 21 and 22
on p.461
461Figures 23 through 26
on p.461
457Figure 1
on p.457
457Figure 2
on p.457

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baenziger N. L., Brodie G. N., Majerus P. W. A thrombin-sensitive protein of human platelet membranes. Proc Natl Acad Sci U S A. 1971 Jan;68(1):240–243. doi: 10.1073/pnas.68.1.240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bak I. J., Hassler R., May B., Westermann E. Morphological and biochemical studies on the storage of serotonin and histamine in blood platelets of the rabbit. Life Sci. 1967 Jun 1;6(11):1133–1146. doi: 10.1016/0024-3205(67)90195-6. [DOI] [PubMed] [Google Scholar]
  3. Bentfeld M. E., Bainton D. F. Cytochemical localization of lysosomal enzymes in rat megakaryocytes and platelets. J Clin Invest. 1975 Dec;56(6):1635–1649. doi: 10.1172/JCI108246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clawson C. C., White J. G. Platelet interaction with bacteria. I. Reaction phases and effects of inhibitors. Am J Pathol. 1971 Nov;65(2):367–380. [PMC free article] [PubMed] [Google Scholar]
  5. Da Prada M., Jakábová M., Lüscher E. F., Pletscher A., Richards J. G. Subcellular localization of the heparin-neutralizing factor in blood platelets. J Physiol. 1976 May;257(2):495–502. doi: 10.1113/jphysiol.1976.sp011380. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Da Prada M., Pletscher A., Tranzer J. P., Knuchel H. Subcellular localization of 5-hydroxytryptamine and histamine in blood platelets. Nature. 1967 Dec 30;216(5122):1315–1317. doi: 10.1038/2161315a0. [DOI] [PubMed] [Google Scholar]
  7. Davis R. B., White J. G. Localization of 5-hydroxytryptamine in blood platelets: an autoradiographic and ultrastructural study. Br J Haematol. 1968 Jul;15(1):93–99. doi: 10.1111/j.1365-2141.1968.tb01515.x. [DOI] [PubMed] [Google Scholar]
  8. Fukami M. H., Bauer J. S., Stewart G. J., Salganicoff L. An improved method for the isolation of dense storage granules from human platelets. J Cell Biol. 1978 May;77(2):389–399. doi: 10.1083/jcb.77.2.389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GORLIN R. J., JUE K. L., JACOBSEN U., GOLDSCHMIDT E. OCULOAURICULOVERTEBRAL DYSPLASIA. J Pediatr. 1963 Nov;63:991–999. doi: 10.1016/s0022-3476(63)80233-4. [DOI] [PubMed] [Google Scholar]
  10. Gerrard J. M., White J. G., Rao G. H. Effects of the lonophore A23187 on the blood platelets II. Influence on ultrastructure. Am J Pathol. 1974 Nov;77(2):151–166. [PMC free article] [PubMed] [Google Scholar]
  11. Marcus A. J., Zucker-Franklin D., Safier L. B., Ullman H. L. Studies on human platelet granules and membranes. J Clin Invest. 1966 Jan;45(1):14–28. doi: 10.1172/JCI105318. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Martin J. H., Carson F. L., Race G. J. Calcium-containing platelet granules. J Cell Biol. 1974 Mar;60(3):775–777. doi: 10.1083/jcb.60.3.775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Moore S., Pepper D. S., Cash J. D. The isolation and characterisation of a platelet-specific beta-globulin (beta-thromboglobulin) and the detection of antiurokinase and antiplasmin released from thrombin-aggregated washed human platelets. Biochim Biophys Acta. 1975 Feb 27;379(2):360–369. doi: 10.1016/0005-2795(75)90143-9. [DOI] [PubMed] [Google Scholar]
  14. Niewiarowski S., Lipiński B., Farbiszewski R., Poplawski A. The release of platelet factor 4 during platelet aggregation and the possible significance of this reaction in hemostasis. Experientia. 1968 Apr 15;24(4):343–344. doi: 10.1007/BF02140810. [DOI] [PubMed] [Google Scholar]
  15. Raccuglia G. Gray platelet syndrome. A variety of qualitative platelet disorder. Am J Med. 1971 Dec;51(6):818–828. doi: 10.1016/0002-9343(71)90311-1. [DOI] [PubMed] [Google Scholar]
  16. Richards J. G., Da Prada M. Uranaffin reaction: a new cytochemical technique for the localization of adenine nucleotides in organelles storing biogenic amines. J Histochem Cytochem. 1977 Dec;25(12):1322–1326. doi: 10.1177/25.12.144762. [DOI] [PubMed] [Google Scholar]
  17. Ross R., Vogel A. The platelet-derived growth factor. Cell. 1978 Jun;14(2):203–210. doi: 10.1016/0092-8674(78)90107-1. [DOI] [PubMed] [Google Scholar]
  18. SOKAL G. [Morphological study of the blood platelets and of viscous metamorphosis using antifibrinogen and antiplatelet fluorescent antiserums]. Acta Haematol. 1962;28:313–325. doi: 10.1159/000207294. [DOI] [PubMed] [Google Scholar]
  19. Salganicoff L., Hebda P. A., Yandrasitz J., Fukami M. H. Subcellular fractionation of pig platelets. Biochim Biophys Acta. 1975 Apr 7;385(2):394–411. doi: 10.1016/0304-4165(75)90369-4. [DOI] [PubMed] [Google Scholar]
  20. White J. G. A search for the platelet secretory pathway using electron dense tracers. Am J Pathol. 1970 Jan;58(1):31–49. [PMC free article] [PubMed] [Google Scholar]
  21. White J. G., Conard W. J. The fine structure of freeze-fractured blood platelets. Am J Pathol. 1973 Jan;70(1):45–56. [PMC free article] [PubMed] [Google Scholar]
  22. White J. G. Fine structural alterations induced in platelets by adenosine diphosphate. Blood. 1968 May;31(5):604–622. [PubMed] [Google Scholar]
  23. White J. G. Interaction of membrane systems in blood platelets. Am J Pathol. 1972 Feb;66(2):295–312. [PMC free article] [PubMed] [Google Scholar]
  24. White J. G. The dense bodies of human platelets. Origin of serotonin storage particles from platelet granules. Am J Pathol. 1968 Nov;53(5):791–808. [PMC free article] [PubMed] [Google Scholar]
  25. Witte L. D., Kaplan K. L., Nossel H. L., Lages B. A., Weiss H. J., Goodman D. S. Studies of the release from human platelets of the growth factor for cultured human arterial smooth muscle cells. Circ Res. 1978 Mar;42(3):402–409. doi: 10.1161/01.res.42.3.402. [DOI] [PubMed] [Google Scholar]
  26. Wood J. G. Electron microscopic localization of 5-hydroxytryptamine (5HT). Tex Rep Biol Med. 1965 Winter;23(4):828–837. [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES